Isolation and Characterisation of a Molybdenum-reducing and Metanil Yellow Dye-decolourising Bacillus sp. strain Neni-10 in Soils from West Sumatera, Indonesia

Mansur, Rusnam; Gusmanizar, Neni; Roslan, Muhamad Akhmal Hakim; Ahmad, Siti Aqlima; Shukor, Mohd Yunus

doi:10.21315/tlsr2017.28.1.5

Cited by 28 publications

(30 citation statements)

References 44 publications

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“…Based on previous studies, the phosphate concentration required is around 5 mM and lower for optimal Mo-reduction [13,15,18,23,28,[30][31][32][33][34][35][36][37]39]. The result of the present study showed that maximum concentration of phosphate also occurred at 5.0 mM.…”

Section: Effect Of Phosphate and Molybdate Concentration On Moreductionsupporting

confidence: 58%

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Sabullah

Rahman

Ahmad

et al. 2017

J. Math. Fund. Sci.

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Abstract. One of the most economical approaches for removal of toxic compounds is bioremediation. In the long term, bioremediation is economic and feasible compared to other methods, such as physical or chemical methods. A bacterium that can efficiently reduce molybdenum blue was isolated from polluted soil. Biochemical analysis revealed the identity of the bacterium as Enterobacter sp. strain Saw-1. The growth parameters for optimal reduction of molybdenum to Mo-blue or molybdenum blue, a less toxic product, were determined around pH 6.0 to 6.5 and in the range of 30 to 37 ℃, respectively. Glucose was selected as preferred carbon source, followed by sucrose, maltose, l-rhamnose, cellobiose, melibiose, raffinose, d-mannose, lactose, glycerol, dadonitol, d-mannitol, l-arabinose and mucate. Phosphate and molybdate were critically required at 5.0 mM and 10 mM, respectively. The scanning absorption spectrum acquired to detect the development of complex Mo-blue showed similarity to previously isolated Mo-reducing bacteria. In addition, the spectrum closely resembled the molybdenum blue from the phosphate determination method. Heavy metals, including mercury, copper (II) and silver (I), inhibited reduction. Moreover, the bacterium also showed capability of exploiting the pesticide coumaphos as an alternative carbon source for growth. As the bacterium proved its ability to detoxify organic and inorganic xenobiotics, the usefulness of this microorganism for bioremediation is highlighted.

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Section: Effect Of Phosphate and Molybdate Concentration On Moreductionsupporting

confidence: 58%

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Sabullah

Rahman

Ahmad

et al. 2017

J. Math. Fund. Sci.

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“…strain Zeid 14 and Burkholderia sp. strain NENI-11 could utilize acrylamide as a source of electron donor for reduction and were able to grow on media containing acrylamide, acetamide, and acetonitrile ( Mansur et al, 2016 ; Mohd et al, 2016 ). Enterobacter sp.…”

Section: Resultsmentioning

confidence: 99%

“…strain Saw-1 and Bacillus sp. strain Neni-12 could consume the pesticide coumaphos as an alternative carbon source for growth ( Sabullah et al, 2016 ; Rusnam and Gusmanizar, 2019 ). However, a previous study found that although some molybdenum-reducing bacteria can successfully utilize pesticides as a carbon source, those pesticides cannot be used as an electron donor for molybdenum reduction ( Sabullah et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Plant Growth and Drought Tolerance-Promoting Bacterium for Bioremediation of Paraquat Pesticide Residues in Agriculture Soils

et al. 2021

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Thailand is an agricultural country. However, agricultural productivity relies on the heavy use of herbicides, especially paraquat. Paraquat accumulation is emerging as a problem in an ever-growing portion of agricultural land. Paraquat residues are toxic to plants, animals, and aquatic organisms in the environment. Biological remediation is a process that can mitigate agricultural chemical contaminants. One of the interesting bioremediators is bacteria. Not only do certain soil bacteria remediate paraquat, but some of them also possess plant growth-promoting properties, which provide advantages in field application. Thus, this study aimed to screen soil bacteria that could degrade paraquat and, at the same time, promote plant growth. Bacteria were isolated from paraquat-treated agricultural soil in Mueang Kaen Pattana municipality, Chiang Mai province, Thailand. On the basis of morphological and 16S rDNA sequence analyses, the selected bacterium was identified as Bacillus aryabhattai strain MoB09. It is capable of growing in nitrogen-free media. B. aryabhattai growth and paraquat degradation were found to be optimum at pH 7 and 30°C. This selected strain also possessed plant growth-promoting abilities, including indole production, siderophore production, phosphate solubilization, and 1-aminocyclopropane-1-carboxylic acid deaminase activity. Paraquat degradation was also evaluated in pot experiments of cowpea (Vigna unguiculata). It was found that this strain could remediate the paraquat residue in both sterilized and non-sterilized soils. The cowpea plants grown in paraquat-contaminated soil with B. aryabhattai showed longer root and shoot lengths than those grown in soil without bacterial inoculation. In addition, B. aryabhattai also promoted the growth of cowpea under induced drought stress. These results suggested that B. aryabhattai could be applied to mitigate paraquat residue in soil and also to promote plant productivity for the organic crop production.

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“…Thus far, the only known Burkholderia genus able to reduce Mo is Burkholderia sp. strain Neni-11 (Mansur et al 2016) with an optimum pH of between 6.0 and 6.3. Having a wide range of optimum pH range for Burkholderia vietnamiensis strain AQ5-12 and Burkholderia sp.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of the simultaneous molybdenum reduction and glyphosate degradation by Burkholderia vietnamiensis AQ5-12 and Burkholderia sp. AQ5-13

et al. 2018

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In this novel study, we report on the use of two molybdenum-reducing bacteria with the ability to utilise the herbicide glyphosate as the phosphorus source. The bacteria reduced sodium molybdate to molybdenum blue (Mo-blue), a colloidal and insoluble product, which is less toxic. The characterisation of the molybdenum-reducing bacteria was carried out using resting cells immersed in low-phosphate molybdenum media. Two glyphosate-degrading bacteria, namely AQ5-12 and sp. AQ5-13, were able to use glyphosate as a phosphorous source to support molybdenum reduction to Mo-blue. The bacteria optimally reduced molybdenum between the pHs of 6.25 and 8. The optimum concentrations of molybdate for strain AQ5-12 was observed to be between 40 and 60 mM, while for sp. AQ5-13, the optimum molybdate concentration occurred between 40 and 50 mM. Furthermore, 5 mM of phosphate was seen as the optimum concentration supporting molybdenum reduction for both bacteria. The optimum temperature aiding Mo-blue formation ranged from 30 to 40 °C for AQ5-12, whereas for sp. AQ5-13, the range was from 35 to 40 °C. Glucose was the best electron donor for supporting molybdate reduction, followed by sucrose, fructose and galactose for both strains. Ammonium sulphate was the best nitrogen source in supporting molybdenum reduction. Interestingly, increasing the glyphosate concentrations beyond 100 and 300 ppm for AQ5-12 and sp. AQ5-13, respectively, significantly inhibited molybdenum reduction. The ability of these bacteria to reduce molybdenum while degrading glyphosate is a useful process for the bioremediation of both toxicants.

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Isolation and Characterisation of a Molybdenum-reducing and Metanil Yellow Dye-decolourising Bacillus sp. strain Neni-10 in Soils from West Sumatera, Indonesia

Cited by 28 publications

References 44 publications

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Plant Growth and Drought Tolerance-Promoting Bacterium for Bioremediation of Paraquat Pesticide Residues in Agriculture Soils

Characterisation of the simultaneous molybdenum reduction and glyphosate degradation by Burkholderia vietnamiensis AQ5-12 and Burkholderia sp. AQ5-13

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