Isolation and characterisation of cancer stem cells from canine osteosarcoma

Wilson, Heather M.; Huelsmeyer, M. K.; Chun, Ruthanne; Young, Karen M.; Friedrichs, Kristen R.; Argyle, David

doi:10.1016/j.tvjl.2007.07.025

Cited by 98 publications

(106 citation statements)

References 17 publications

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“…As Gibbs and Wilson reported (Gibbs et al, 2005;Wilson et al, 2008), the MG-63 cell line formed a recognized sarcospheres that could be observed microscopically after cultivation in serum-free medium for 7~10 days ( Figure 1A). We evaluated the proportion of the cell that can be form sarcosphere in MG-63 cell line preliminarily, which was close to the correlational researches (Gibbs et al, 2005;Meyers et al, 2005;Dick, 2008;Visvader and Lindeman, 2008;Wilson et al, 2008).…”

Section: Isolation Of Sarcospheres From Osteosarcoma Cell Lines Mg-63supporting

confidence: 60%

“…We evaluated the proportion of the cell that can be form sarcosphere in MG-63 cell line preliminarily, which was close to the correlational researches (Gibbs et al, 2005;Meyers et al, 2005;Dick, 2008;Visvader and Lindeman, 2008;Wilson et al, 2008). The cells of sarcospheres could also generate the secondary cell spheres in serum-free medium as Gibbs described (Gibbs et al, 2005).…”

Section: Isolation Of Sarcospheres From Osteosarcoma Cell Lines Mg-63mentioning

confidence: 63%

“…Gibbs and Wilson firstly isolated the CSCs from human and canine osteosarcoma cell lines, and identified their stemness by studying biological properties and applying respective surface markers (Gibbs et al, 2005;Wilson et al, 2008). The CSCs from osteosarcoma demonstrated more active features in proliferation and self-renewal than normal cancer cell, which also expressed many stem cell markers such as CD44, CD105, Oct-3/4 and Nanog, etc (Gibbs et al, 2005;Wilson et al, 2008).…”

Section: Ly294002 Induces G0/g1 Cell Cycle Arrest and Apoptosis Of Camentioning

confidence: 99%

“…The CSCs from osteosarcoma demonstrated more active features in proliferation and self-renewal than normal cancer cell, which also expressed many stem cell markers such as CD44, CD105, Oct-3/4 and Nanog, etc (Gibbs et al, 2005;Wilson et al, 2008). Recently, Tirion and Veselska isolated CD133+ population from human osteosarcoma cell lines MG-63, Saos-2 and U2OS, and demonstrated CD133+ cells might be used as markers for identifying osteosarcoma CSCs (Tirino et al, 2008;Veselska et al, 2008).…”

Section: Ly294002 Induces G0/g1 Cell Cycle Arrest and Apoptosis Of Camentioning

confidence: 99%

“…The process of osteosarcoma CSCs isolation was mostly carrying out as previously described (Gibbs et al, 2005;Wilson et al, 2008). In brief, cells were harvested by trypsinization and cells number was counted afterwards.…”

Section: Isolation and Passages Of Osteosarcoma Cscsmentioning

confidence: 99%

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LY294002 Induces G0/G1 Cell Cycle Arrest and Apoptosis of Cancer Stem-like Cells from Human Osteosarcoma Via Down-regulation of PI3K Activity

Chen¹,

Liao²,

Wang³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Asian Pacific J Cancer Prev, 13,[3103][3104][3105][3106][3107] IntroductionOsteosarcoma is the most common primary mesenchymal malignant tumor of bone tissue in human, especially in children and adolescents, which tends to metastasize early with serious outcome and is more often in the metaphyseal areas of long bone such as distal femur, proximal tibia and humerus. Long-term survival rate increases from 25% to 65% with advances in surgical techniques and chemotherapy, but this figure has no obvious improvement in many years. Investigation of its reason is many sided and the main aspect is drug-resistance during chemotherapy (Meyers et al., 2005).The tumorigenic mechanism of osteosarcoma and its respective drug-resistance are barely understood. In the past one to two decades, more and more studies suggest that some tumors are hierarchically organized, which means that these tumor cells maintains differences in their morphology, function and differentiation level (Hanahan and Weinberg, 2000;Dick, 2008). Based on the similarity shared between these tumors, many researchers have turned to a new theory, the CSCs theory, which could help people explain tumorigenesis and drug-resistance better (Dick, 2008;Visvader and Lindeman, 2008

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Section: Isolation Of Sarcospheres From Osteosarcoma Cell Lines Mg-63supporting

confidence: 60%

Section: Isolation Of Sarcospheres From Osteosarcoma Cell Lines Mg-63mentioning

confidence: 63%

Section: Ly294002 Induces G0/g1 Cell Cycle Arrest and Apoptosis Of Camentioning

confidence: 99%

Section: Ly294002 Induces G0/g1 Cell Cycle Arrest and Apoptosis Of Camentioning

confidence: 99%

Section: Isolation and Passages Of Osteosarcoma Cscsmentioning

confidence: 99%

See 3 more Smart Citations

LY294002 Induces G0/G1 Cell Cycle Arrest and Apoptosis of Cancer Stem-like Cells from Human Osteosarcoma Via Down-regulation of PI3K Activity

Chen¹,

Liao²,

Wang³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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show abstract

Multiplexing High‐Content Flow (HCF) and Quantitative High‐Throughput Screening (qHTS) to Identify Compounds Capable of Decreasing Cell Viability, Activating Caspase 3/7, Expressing Annexin V, and Changing Mitochondrial Membrane Integrity

Mathews

Keller

McKnight

et al. 2013

CP Chemical Biology

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High‐content flow (HCF) screening systems, such as the iQue Screener and HTFC Screening System from IntelliCyt, have facilitated the implementation of flow cytometry assays for high‐throughput screening. HCF screening systems enable the use of smaller sample volumes and multiplexed assays to simultaneously assess different cellular parameters from a single well. This becomes invaluable when working with cells or compounds that are available in limited quantities or when conducting large‐scale screens. When assays can be miniaturized to a 384‐ or 1536‐well microplate format, it is possible to implement dose‐response‐based high‐throughput screens, also known as quantitative HTS or qHTS. This article describes how qHTS at the new National Center for Advancing Translational Science (NCATS) has been systematically coupled with the HTFC Screening System and Multimetric Apoptosis Screening Kit from IntelliCyt to biologically validate active compounds from primary cell proliferation screens using a model of diffuse large B cell lymphoma (DLBCL). Curr. Protoc. Chem. Biol. 5:195‐212 © 2013 by John Wiley & Sons, Inc.

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Stem Cell Biology

Borjesson¹,

Overmann²

2022

Schalm's Veterinary Hematology

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Isolation and characterisation of cancer stem cells from canine osteosarcoma

Cited by 98 publications

References 17 publications

LY294002 Induces G0/G1 Cell Cycle Arrest and Apoptosis of Cancer Stem-like Cells from Human Osteosarcoma Via Down-regulation of PI3K Activity

LY294002 Induces G0/G1 Cell Cycle Arrest and Apoptosis of Cancer Stem-like Cells from Human Osteosarcoma Via Down-regulation of PI3K Activity

Multiplexing High‐Content Flow (HCF) and Quantitative High‐Throughput Screening (qHTS) to Identify Compounds Capable of Decreasing Cell Viability, Activating Caspase 3/7, Expressing Annexin V, and Changing Mitochondrial Membrane Integrity

Stem Cell Biology

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