Isolation and characterisation of cytotoxic compounds from <i>Euphorbia clementei</i> Boiss.

Benmerache, Abbes; Magid, Abdulmagid Alabdul; Labed, Amira; Kabouche, Ahmed; Voutquenne-Nazabadioko, Laurence; Hubert, Jane; Morjani, Hamid; Kabouche, Zahia

doi:10.1080/14786419.2016.1277345

Cited by 11 publications

(5 citation statements)

References 30 publications

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“…Co-TLC analyses of the extracts along with common phytochemicals 1, 2, 3 and 4 were performed using silica gel (Figure 6, (1)) and AgNO3 -silica gel stationary phases (Figure 6, (2), ( 3)). In literature, compound 1 and 4 showed antioxidant and cytotoxic activity, while compound 3 showed antibacterial activity (20)(21)(22)(23)(24). Compounds 1 and 2 are present in almost all extracts in different ratios (Figure 6 (1), ( 3)), while the presence of compound 3 was confirmed in the MeOH extracts of the shoots of E. microsciadia, the roots of E. macrostegia, 80% MeOH extracts of the roots of E. microsciadia and the shoots of E. macrostegia and all the DCM extracts.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant, Cytotoxic and Antibacterial Activity and Total Phenol Contents of the Roots and the Shoots of Euphorbia macrostegia and Euphorbia microsciadia

2020

J. Environ. Treat. Tech.

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The roots and the shoots of Euphorbia macrostegia and E. microsciadia were extracted using different solvents; dichloromethane (DCM), methanol (MeOH) and MeOH: water (80:20) and the extracts were screened for their cytotoxic, antioxidant and antimicrobial properties as well as total phenolic content. Cytotoxicity of the extracts was evaluated against human acute lymphoblastic leukemia (MOLT-4) cells by MTT reduction assay. The extracts were also subjected to the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and Folin-Ciocalteu total phenolic assays. The MeOH extract of the shoots of E. microsciadia and DCM extract of the roots of E. macrostegia were the most cytotoxic ones with IC50 values of 10.5 ± 2.6 and 7.0 ± 1.2 µg/mL, respectively. The MeOH extract of the shoots of E. microsciadia showed considerable antioxidant activity (IC50 = 9.95 ± 1.00 µg plant extracted to scavenge 1 mL of a 0.1 mM DPPH solution), which was consistent with its highest phenolic content (288.50 ± 29.38 mg equivalent of gallic acid in 1g dry plant material: mg EG/g PM). Determination of minimum inhibitory concentrations (MICs) using the broth dilution method, confirmed that all the extracts from the plants gave various degrees of antibacterial activity against all tested microorganisms. In thin layer chromatography (TLC) investigations, some compounds previously isolated from Euphorbia species inclduing cycloartenol, 24-methylenecycloartan-3β-ol, β-sitosterol and euphol were tested and suggested to be responsible for the above-mentioned biological activities in the plants. Therefore, we suggest E. macrostegia and especially E. microsciadia as new sources for isolation and identification of various bioactive compounds.

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Section: Resultsmentioning

confidence: 99%

Antioxidant, Cytotoxic and Antibacterial Activity and Total Phenol Contents of the Roots and the Shoots of Euphorbia macrostegia and Euphorbia microsciadia

2020

J. Environ. Treat. Tech.

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“…CT = Weak cytotoxic activity against HL60 cell line and p38MAPK-mediated apoptosis in the U87 cell line. [91,[194][195][196][197][198][199][200] Betulinic…”

Section: Triterpenes From Mexican Columnar Cactaceaementioning

confidence: 99%

Chemistry, Biological Activities and In Silico Bioprospection of Sterols and Triterpenes from Mexican Columnar Cactaceae

2020

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The Cactaceae family is an important source of triterpenes and sterols. The wide uses of those plants include food, gathering, medicinal, and live fences. Several studies have led to the isolation and characterization of many bioactive compounds. This review is focused on the chemistry and biological properties of sterols and triterpenes isolated mainly from some species with columnar and arborescent growth forms of Mexican Cactaceae. Regarding the biological properties of those compounds, apart from a few cases, their molecular mechanisms displayed are not still fully understand. To contribute to the above, computational chemistry tools have given a boost to traditional methods used in natural products research, allowing a more comprehensive exploration of chemistry and biological activities of isolated compounds and extracts. From this information an in silico bioprospection was carried out. The results suggest that sterols and triterpenoids present in Cactaceae have interesting substitution patterns that allow them to interact with some bio targets related to inflammation, metabolic diseases, and neurodegenerative processes. Thus, they should be considered as attractive leads for the development of drugs for the management of chronic degenerative diseases.

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“…Cyclobutane is an example of a four-membered cyclic compound. Many naturally occurring compounds contain cyclobutane scaffolds, including katsumadain C, palhinosides A–H, methylcyclobutene, and cyclobutene-based sesquiterpenes . Moreover, the derivatives of cyclobutanes serve as valuable building blocks in relation to organic synthesis .…”

Section: Introductionmentioning

confidence: 99%

Substrate-Controlled Regioselective Bromination of 1,2-Disubstituted Cyclobutenes: An Application in the Synthesis of 2,3-Disubstituted Cyclobutenones

et al. 2021

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Easily available disubstituted cyclobutenes were regioselectively halogenated at the allylic position by means of a reaction with bromine. The regioselectivity of bromination is controlled by the presence of a carbocation-stabilizing group. The prepared disubstituted 3-bromocyclobutenes were converted into the corresponding disubstituted cyclobutenones. On the basis of the performed experiments, the mechanism behind the bromination reaction was also proposed.

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Isolation and characterisation of cytotoxic compounds from Euphorbia clementei Boiss.

Cited by 11 publications

References 30 publications

Antioxidant, Cytotoxic and Antibacterial Activity and Total Phenol Contents of the Roots and the Shoots of Euphorbia macrostegia and Euphorbia microsciadia

Antioxidant, Cytotoxic and Antibacterial Activity and Total Phenol Contents of the Roots and the Shoots of Euphorbia macrostegia and Euphorbia microsciadia

Chemistry, Biological Activities and In Silico Bioprospection of Sterols and Triterpenes from Mexican Columnar Cactaceae

Substrate-Controlled Regioselective Bromination of 1,2-Disubstituted Cyclobutenes: An Application in the Synthesis of 2,3-Disubstituted Cyclobutenones

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