Isolation and characterisation of fungi growing on volatile aromatic hydrocarbons as their sole carbon and energy source

Prenafeta-Boldú, Francesc X.; Kuhn, Andrea; Luykx, Dion M.A.M.; Anke, Heidrun; Groenestijn, J.W. van; Bont, J.A.M. de

doi:10.1017/s0953756201003719

Cited by 125 publications

(121 citation statements)

References 31 publications

Supporting

Mentioning

114

Contrasting

Unclassified

Order By: Relevance

“…Some Chaetothyriales are abundant in humid indoor habitats (Matos et al 2002;Döğen et al 2013), but other species, by contrast, colonize harsh environments where they have to cope with drought, scarce nutrient availability, extreme temperatures and high UV-light exposure Tsuneda et al 2011). This order has also received special attention due to the recent discovery of mutualistic interactions with ant species (Voglmayr et al 2011;Nepel et al 2014), and to the ability of certain species to degrade aromatic hydrocarbon contaminants (Prenafeta-Boldú et al 2001;Badali et al 2011). …”

Section: Introductionmentioning

confidence: 99%

“…Therefore, special isolation techniques may be necessary. Previous authors have successfully implemented methods using experimental inoculations in laboratory animals (Gezuele et al 1972;Dixon et al 1980), high incubation temperatures (Sudhadham et al 2008), extraction with mineral oil (Satow et al 2008;Vicente et al 2008), enrichment on atmospheres with aromatic hydrocarbons (Prenafeta-Boldú et al 2001;Zhao et al 2010) and media containing cycloheximide (Salgado et al 2004). In our experience, media with cycloheximide proved to be very useful to isolate members of the Chaetothyriales.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

New and interesting chaetothyrialean fungi from Spain

et al. 2016

View full text Add to dashboard Cite

In the course of taxonomic studies on saprobic microfungi from Spain, several slow-growing, dematiaceous hyphomycetes were isolated from soil, submerged plant material and river sediments. Sixteen of these strains were identified as members of the ascomycete order Chaetothyriales on the basis of morphology and DNA sequence analyses of the internal transcribed spacer region and partial large subunit ribosomal RNA gene. These included three novel species (Cladophialophora pseudocarrionii, Cyphellophora chlamydospora, and Rhinocladiella amoena) and five interesting, little-known or clinically-relevant species (Cyphellophora suttonii, Exophiala aquamarina, E. lacus, E. radicis, and Rhinocladiella similis). In addition, Exophiala oligosperma, an emerging opportunistic fungus, was found for the first time in an aquatic freshwater environment (river sediments). Cladophialophora pseudocarrionii resembles C. carrionii in the branching pattern of its conidial chains, but differs from the latter species in its inability to grow at 30 °C. Cyphellophora chlamydospora differs from other species of the genus in the absence of conidiation, producing only chlamydospores in vitro. Rhinocladiella amoena shows branched conidiophores similar to those of R. anceps, R. atrovirens, R. basitona and R. similis, but differs from them in conidial shape and size. The ex-type strain of Phialophora livistonae, included in the phylogenetic study, clustered with high statistical support with members of the genus Cyphellophora and is transferred to this genus.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New and interesting chaetothyrialean fungi from Spain

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Their environmental counterparts usually live in extreme or toxic environments (3)(4)(5). However, only recently we began to realize that black yeast-like fungi are all around in outdoor and indoor, man-made environments, such as railways, bathrooms (6,7), steam baths (8), and dishwashers (9).…”

mentioning

confidence: 99%

Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

et al. 2013

View full text Add to dashboard Cite

hThe species diversity and identification of black fungi belonging to Cyphellophora and Phialophora, which colonize and infect human skin and nails, were studied using amplified fragment length polymorphism (AFLP). A total of 76 Cyphellophora and Phialophora isolates were evaluated, and their delimitation was compared to earlier studies using multilocus sequencing. The results of the AFLP analysis and sequencing were in complete agreement with each other. Seven species-specific padlock probes for the most prevalent species were designed on the basis of the ribosomal DNA internal transcribed spacer region, and identification of the respective species could easily be achieved with the aid of rolling circle amplification.

show abstract

“…The 19 F isotope, with a natural abundance of 100% and a broad chemical shift range, is a very sensitive NMR-active nucleus that can be advantageously used in the identification and quantification of fluorinated intermediates by 19 F NMR spectroscopy. Our objectives were to identify the initial steps for the catabolism of toluene in six previously isolated fungi (14,20) which are capable of growth on toluene as a sole carbon and energy source: C. sphaerospermum T0, Cladophialophora sp. strains T1 and T2, Pseudeurotium zonatum T3, Exophiala sp.…”

mentioning

confidence: 99%

“…Oxygen consumption experiments with whole cells and enzyme activities in cell extracts suggest that the initial oxidation of toluene takes place at the methyl group, rather than at the aromatic ring. Recently, we identified five additional fungi that also can assimilate toluene (14). 19 F nuclear magnetic resonance ( 19 F NMR) has been used previously to characterize the degradation of fluorine-containing aromatic compounds by fungi (2,13,18).…”

mentioning

confidence: 99%

Fungal Metabolism of Toluene: Monitoring of Fluorinated Analogs by ¹⁹ F Nuclear Magnetic Resonance Spectroscopy

Prenafeta-Boldú

Luykx

Vervoort³

et al. 2001

Appl Environ Microbiol

View full text Add to dashboard Cite

We used isomeric fluorotoluenes as model substrates to study the catabolism of toluene by five deuteromycete fungi and one ascomycete fungus capable of growth on toluene as the sole carbon and energy source, as well as by two fungi (Cunninghamella echinulata and Aspergillus niger) that cometabolize toluene. Whole cells were incubated with 2-, 3-, and 4-fluorotoluene, and metabolites were characterized by 19 F nuclear magnetic resonance. Oxidation of fluorotoluene by C. echinulata was initiated either at the aromatic ring, resulting in fluorinated o-cresol, or at the methyl group to form fluorobenzoate. The initial conversion of the fluorotoluenes by toluene-grown fungi occurred only at the side chain and resulted in fluorinated benzoates. The latter compounds were the substrate for the ring hydroxylation and, depending on the fluorine position, were further metabolized up to catecholic intermediates. From the 19 F nuclear magnetic resonance metabolic profiles, we propose that diverse fungi that grow on toluene assimilate toluene by an initial oxidation of the methyl group.In bacteria, five different metabolic pathways for the complete aerobic degradation of toluene and its assimilation are known (9, 16, 22, 23; B. Kaphammer, J. J. Kukor, and R. H. Olsen, Abstr. 90th Annu. Meet. Am. Soc. Microbiol., abstr. K-145, p. 243, 1990). Depending upon the strain, toluene is initially oxidized either at the methyl group or at the aromatic ring. Fungi also can oxidize toluene at both molecular sites. Cultures of Mortierella isabellina converted toluene into benzyl alcohol (12). Smith and Rosazza (17) identified two zygomycetes and three deuteromycetes that hydroxylated toluene at the aromatic ring to produce o-cresol and, in some cases, p-cresol. Mineralization of toluene has been reported for the white-rot fungus Phanerochaete chrysosporium, but the metabolic pathway was not determined (25). The fungal degradation of toluene in these cases occurred only by cometabolism and, consequently, it did not support growth. We previously identified and described a Cladosporium sphaerospermum strain that can grow on toluene as the sole carbon and energy source (20). Oxygen consumption experiments with whole cells and enzyme activities in cell extracts suggest that the initial oxidation of toluene takes place at the methyl group, rather than at the aromatic ring. Recently, we identified five additional fungi that also can assimilate toluene (14).19 F nuclear magnetic resonance ( 19 F NMR) has been used previously to characterize the degradation of fluorine-containing aromatic compounds by fungi (2, 13, 18). Fluorine, with its small size, can replace hydrogen in an organic substrate with few steric consequences. It also influences the conversion rate of many enzyme reactions (21). The 19 F isotope, with a natural abundance of 100% and a broad chemical shift range, is a very sensitive NMR-active nucleus that can be advantageously used in the identification and quantification of fluorinated intermediates by 19 F NMR spectroscopy. Our obj...

show abstract

Isolation and characterisation of fungi growing on volatile aromatic hydrocarbons as their sole carbon and energy source

Cited by 125 publications

References 31 publications

New and interesting chaetothyrialean fungi from Spain

New and interesting chaetothyrialean fungi from Spain

Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

Fungal Metabolism of Toluene: Monitoring of Fluorinated Analogs by ¹⁹ F Nuclear Magnetic Resonance Spectroscopy

Contact Info

Product

Resources

About

Isolation and characterisation of fungi growing on volatile aromatic hydrocarbons as their sole carbon and energy source

Cited by 125 publications

References 31 publications

New and interesting chaetothyrialean fungi from Spain

New and interesting chaetothyrialean fungi from Spain

Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

Fungal Metabolism of Toluene: Monitoring of Fluorinated Analogs by 19 F Nuclear Magnetic Resonance Spectroscopy

Contact Info

Product

Resources

About

Fungal Metabolism of Toluene: Monitoring of Fluorinated Analogs by ¹⁹ F Nuclear Magnetic Resonance Spectroscopy