2017
DOI: 10.1071/zo17053
|View full text |Cite
|
Sign up to set email alerts
|

Isolation and characterisation of microsatellites for the endangered Slater’s skink, Liopholis slateri (Squamata : Scincidae), via next-generation sequencing

Abstract: We characterised 14 new polymorphic microsatellite loci for the endangered lizard Liopholis slateri. Initially, 454 shotgun sequencing was used to identify 46 loci, which were trialled for amplification. Subsequently, 14 of these loci were screened for variation in 21 individuals from scat-derived DNA samples collected from Owen Springs Reserve in central Australia. All 14 loci were polymorphic, with observed heterozygosity ranging from 0.19 to 0.86 and the number of alleles per locus ranging from 2 to 10. The… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2018
2018
2019
2019

Publication Types

Select...
2

Relationship

0
2

Authors

Journals

citations
Cited by 2 publications
(1 citation statement)
references
References 10 publications
0
1
0
Order By: Relevance
“…using microsatellites generated via shotgun sequencing; see Gardner et al . ). Unfortunately, to do so requires a nondestructive and non‐lethal tissue sampling procedure, which remains a frustratingly prohibitive (yet necessary) challenge for mygalomorph ecology and systematics.…”
Section: Discussionmentioning
confidence: 97%
“…using microsatellites generated via shotgun sequencing; see Gardner et al . ). Unfortunately, to do so requires a nondestructive and non‐lethal tissue sampling procedure, which remains a frustratingly prohibitive (yet necessary) challenge for mygalomorph ecology and systematics.…”
Section: Discussionmentioning
confidence: 97%