Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae

Osborn, Rupert W.; Samblanx, Genoveva W. De; Thevissen, Karin; Goderis, Inge J.W.M.; Torrekens, Sophie; Leuven, Fred Van; Attenborough, Sheila; Rees, Sarah B.; Broekaert, Willem F.

doi:10.1016/0014-5793(95)00666-w

Cited by 368 publications

(297 citation statements)

References 22 publications

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“…However, subsequent work has established that thionins and y-thionins are structurally unrelated (Terras et al, 1992;Bruix et al, 1993;Bohlmann, 1994). Proteins homologous to "y-thionins" have been isolated from seeds of various monocot and dicot species (Bloch and Richardson, 1991;Terras et al, 1992Terras et al, , 1993Moreno et al, 1994;Osborn et al, 1995) Vol. 108, 1995Vol.…”

Section: Plant Defensins Share Structural Properties Wlth Mammalian Amentioning

confidence: 99%

“…The "morphogenic" plant defensins cause reduced hyphal elongation with a concomitant increase in hyphal branching. The plant defensins from the Brassicaceae (including Rs-AFP1 and Rs-AFP2 from radish seeds) and Saxifragaceae (including Hs-AFPI from Heuchera sanguinea seeds) belong to this group (Terras et al, 1992(Terras et al, , 1993Osborn et al, 1995). On the other hand, the plant defensins from the Asteraceae (including DmAMPl from dahlia seeds), the Fabaceae (including CtAMPl from Clitoria tevnatea seeds), and the Hippocastanaceae (including Ah-AMP1 from horse chestnut seeds) slow down hyphal extension but do not induce marked morphological distortions.…”

Section: Nicoliuna Iabacum (Fst) F Rec-ktesntfpgicitkppcrkacis-ek-ftdmentioning

confidence: 99%

“…The antifungal activity of plant defensins, whether morphogenic or not, is reduced by increasing the ionic strength of the fungal growth assay medium. However, the antagonistic effect of ions is strongly dependent on the fungus and thus on the conformation of the putative target site (Terras et al, 1992;Osborn et al, 1995). Ionic strength antagonism was found to be due to cations, with divalent cations being at least 1 order of magnitude more potent than monovalent cations (Terras et al, 1992(Terras et al, , 1993.…”

Section: Nicoliuna Iabacum (Fst) F Rec-ktesntfpgicitkppcrkacis-ek-ftdmentioning

confidence: 99%

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Plant Defensins: Novel Antimicrobial Peptides as Components of the Host Defense System

Broekaert¹,

Terras²,

Cammue³

et al. 1995

Plant Physiol.

685

431

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Various mechanisms to fend off microbial invaders have been devised by a11 living organisms, including microorganisms themselves. The most sophisticated of these mechanisms relies on the synthesis of immunoglobulins directed against specific microbial targets. However, immunoglobulin-based immunity operates only in a relatively minor subset of living species, namely the higher vertebrates. A much more ancient and widespread defense strategy involves the production of small peptides that exert antimicrobial properties. As products of single genes, antimicrobial peptides can be synthesized in a swift and flexible way, and because of their small size they can be produced by the host with a minimal input of energy and biomass. Wellknown examples of antimicrobial peptides are the cecropins that accumulate in the hemolymph of many invertebrates in response to injury or infection (reviewed by Boman and Hultmark, 1987) and the magainins that are secreted by glands in the skin of amphibians (reviewed by Bevins and Zasloff, 1990). Cecropins and magainins are small (2040 residues) basic peptides displaying an amphipathic a-helical structure that can integrate in microbial membranes to form ion channels (Duclohier, 1994).Another class of antimicrobial peptides is formed by the Cys-rich peptides, which in contrast to cecropins and magainins, have a complex cystine-stabilized three-dimensional folding pattern often involving antiparallel P-sheets. Defensins are one class among the numerous types of Cys-rich antimicrobial peptides, which differ in length, number of cystine, bonds, or folding pattern (reviewed by Boman, 1995). Insect defensins (3443 residues, three disulfide bridges) are, like cecropins, produced in a pathogeninducible manner by the insect fat body and secreted in the hemolymph (reviewed by Hoffmann and Hétru, 1992).

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Section: Plant Defensins Share Structural Properties Wlth Mammalian Amentioning

confidence: 99%

Section: Nicoliuna Iabacum (Fst) F Rec-ktesntfpgicitkppcrkacis-ek-ftdmentioning

confidence: 99%

Section: Nicoliuna Iabacum (Fst) F Rec-ktesntfpgicitkppcrkacis-ek-ftdmentioning

confidence: 99%

See 1 more Smart Citation

Plant Defensins: Novel Antimicrobial Peptides as Components of the Host Defense System

Broekaert¹,

Terras²,

Cammue³

et al. 1995

Plant Physiol.

685

431

View full text Add to dashboard Cite

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“…To improve the resistance of papaya to Phytophthora, Zhu et al [26] introduced the defensin gene from Dahlia merckii by particle bombardment in embryogenic calli of papaya. The dahlia defensin has been shown to inhibit the in vitro growth of a broad range of fungi [88][89]. The defensin gene has also been introduced in different crops resulting in enhanced resistance to their respective fungal pathogens, e.g., radish defensin in tobacco vs. the leaf pathogen Alternaria longipes [90] and tomato vs. A. solani [91].…”

Section: In Vietnammentioning

confidence: 99%

Recent advances in the development of transgenic papaya technology

Mendoza

Laurena

Botella

2008

Biotechnology Annual Review

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Abstract. Papaya with resistance to papaya ringspot virus (PRSV) is the first genetically modified tree and fruit crop and also the first transgenic crop developed by a public institution that has been commercialized. This chapter reviews the different transformation systems used for papaya and recent advances in the use of transgenic technology to introduce important quality and horticultural traits in papaya. These include the development of the following traits in papaya: resistance to PRSV, mites and Phytophthora, delayed ripening trait or long shelf life by inhibiting ethylene production or reducing loss of firmness, and tolerance or resistance to herbicide and aluminum toxicity. The use of papaya to produce vaccine against tuberculosis and cysticercosis, an infectious animal disease, has also been explored. Because of the economic importance of papaya, there are several collaborative and independent efforts to develop PRSV transgenic papaya technology in 14 countries. This chapter further reviews the strategies and constraints in the adoption of the technology and biosafety to the environment and food safety. Constraints to adoption include public perception, strict and expensive regulatory procedures and intellectual property issues.

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“…However, some bacteria, particularly Grampositive species, are also inhibited, although the activity is less pronounced than that against fungi. The growth of several fungal species, including several filamentous fungi and yeast cells, was inhibited when incubated with these peptides [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. Despite the broad inhibitory activity that plant defensins exhibit against different micro-organisms, scientists know little about their activity against protozoa.…”

Section: Introductionmentioning

confidence: 99%

PvD1 defensin, a plant antimicrobial peptide with inhibitory activity against Leishmania amazonensis

et al. 2015

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SynopsisPlant defensins are small cysteine-rich peptides and exhibit antimicrobial activity against a variety of both plant and human pathogens. Despite the broad inhibitory activity that plant defensins exhibit against different micro-organisms, little is known about their activity against protozoa. In a previous study, we isolated a plant defensin named PvD 1 from Phaseolus vulgaris (cv. Pérola) seeds, which was seen to be deleterious against different yeast cells and filamentous fungi. It exerted its effects by causing an increase in the endogenous production of ROS (reactive oxygen species) and NO (nitric oxide), plasma membrane permeabilization and the inhibition of medium acidification. In the present study, we investigated whether PvD 1 could act against the protozoan Leishmania amazonensis. Our results show that, besides inhibiting the proliferation of L. amazonensis promastigotes, the PvD 1 defensin was able to cause cytoplasmic fragmentation, formation of multiple cytoplasmic vacuoles and membrane permeabilization in the cells of this organism. Furthermore, we show, for the first time, that PvD 1 defensin was located within the L. amazonensis cells, suggesting the existence of a possible intracellular target.

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Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae

Cited by 368 publications

References 22 publications

Plant Defensins: Novel Antimicrobial Peptides as Components of the Host Defense System

Plant Defensins: Novel Antimicrobial Peptides as Components of the Host Defense System

Recent advances in the development of transgenic papaya technology

PvD1 defensin, a plant antimicrobial peptide with inhibitory activity against Leishmania amazonensis

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