Isolation and characteristics of aspartate aminotransferase from boar spermatozoa

Ciereszko, Andrzej; Strzeżek, J.

doi:10.1016/0020-711x(89)90154-7

Cited by 10 publications

(5 citation statements)

References 27 publications

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“…Broad pH optimum found for semen AspAT in our experiment is also characteristic for AspAT of many mammalian tissues, including mammalian semen (Ciereszko and Strzezek 1989). However, the enzyme from fish tissues and serum had shown a rapid decline of activity above pH 7.2 (Cassilas et al 1982;d'Apolonia et al 1980).…”

Section: Days Of Storagementioning

confidence: 96%

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Relationship between biochemical constituents of fish semen and fertility: the effect of short-term storage

Ciereszko

Dąbrowski

1994

Fish Physiol Biochem

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109

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Spermatozoa and seminal plasma obtained from rainbow trout and whitefish were analyzed in respect to their aspartate aminotransferase (AspAT) and alkaline phosphatase activities. In particular, the experiments characterized AspAT optimum pH, optimization of assay conditions and action of coenzyme, pyridoxal 5-phosphate (vitamin B6). The effect of short-term semen storage at 0°C on biochemical indicators and fertilization rate was examined in both species. The concentrations of reduced and oxidized ascorbic acid in seminal plasma of both species were several folds higher than in spermatozoa and blood plasma of fish. Highly significant correlations were found for both species between AspAT activity (sperm or seminal plasma) and fertilization rate (% of eyed-stage or hatched embryos). For rainbow trout, highly significant correlations were found between sperm concentration, motility and fertilization rate. These results suggest that several biochemical indicators of seminal plasma can be used as measures of sperm quality of fish. Some common biochemical parameters for fish and mammal's semen provide evidence for using fish sperm as a model in biomedical research.

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Section: Days Of Storagementioning

confidence: 96%

“…There are two isoenzymes of aspartate aminotransferase in mammalian semen (Elliason 1967;Mortimer et al 1988;Ciereszko and Strzezek 1989), cytoplasmic and mitochondrial. The former one is easily liberated from spermatozoa to seminal plasma (Ciereszko et al 1990).…”

Section: Days Of Storagementioning

confidence: 99%

Relationship between biochemical constituents of fish semen and fertility: the effect of short-term storage

Ciereszko

Dąbrowski

1994

Fish Physiol Biochem

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109

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“…Furthermore, Zn is included in the WHO manual as an indicator of prostatic function [WHO 2010] and low levels of Zn have been found in SP of infertile men [Chia et al 2000;Colagar et al 2009]. In boars, some changes in mineral composition are related to season and heat stress [Larsson and Einarsson 1984;Murase et al 2007] Although several studies are available on these enzymes and minerals in boar semen, their association with semen quality was not clearly demonstrated [Boursnell et al 1973;Ciereszko and Strzezek 1989;Einarsson et al 1970;Lavon and Boursnell 1975;Strzezek et al 1995;Strzezek et al 1998;Wysocki and Strzezek 2006]. Moreover, many of these studies are rather dated.…”

Section: Introductionmentioning

confidence: 99%

Boar seminal plasma components and their relation with semen quality

Rodriguez

Rijsselaere

Beek

et al. 2012

Systems Biology in Reproductive Medicine

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Select boar seminal plasma (SP) components and their relation to semen quality were investigated. Thirty nine boars from three artificial insemination (AI) centers were divided into group A (GA: > 80% normal sperm and >70% motility) and group B (GB: < 80% normal sperm and < 70% motility). Each ejaculate was collected and semen volume, concentration, sperm motility (computer aided semen analysis; CASA), morphology, and vitality (both eosin nigrosin staining) were investigated. The SP was separated and analyzed for aspartate-amino-transferase (AST), γ-glutamyl-transferase (GGT), alkaline phosphatase (ALP) activity, and the concentrations of sodium (Na), potassium (K), chloride (Cl), calcium (Ca), phosphate (PO 4 3-), magnesium (Mg), selenium (Se) and zinc (Zn) were assessed. Repeated measures (2 months interval) were conducted in eight boars of GA from one AI center. The activity of GGT (r = -0.482) and ALP (r = -0.459) was moderately associated ( p < 0.05) with ejaculate volume and strongly associated with concentration (r = 0.580 and r = 0.618, respectively; p = 0.000). Moderate associations ( p < 0.05) were found between ALP (r = 0.439), GGT (r = 0.387), Na (r = -0.428), K (r = 0.354), and Se (r = 0.354) with progressive motility. The SP concentration of Na (r = -0.401), Cl (r = -0.521), and K (r = 0.350) was associated ( p < 0.05) with normal morphology. Only Mg was associated ( p < 0.05) with membrane damage (r = -0.335). The concentration of Na, Cl, and Zn (1681.0 vs. 1701.0 µg/dL) was different between groups ( p < 0.05). Repeated measures showed significant differences in time but only for Na, Mg, and Zn ( p < 0.05). In conclusion, several biochemical components of SP were related to semen quality. The analysis of biochemical parameters could provide extra information about reproductive health of AI boars.

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“…The gradual increase in the percentage of SPMI was negatively correlated with AAT activity in seminal plasma. This relationship could suggest that changes in plasma membrane permeability induced by various factors, including frequent ejaculation, could promote AAT leakage to the plasma (Ciereszko and Strzeżek 1989). Therefore, a decrease in AAT activity on successive days of ejaculate collection could point to a gradual increase in SPMI on successive days of the experiment.…”

Section: Discussionmentioning

confidence: 99%

Effect of repeated semen ejaculation on sperm quality and selected biochemical markers of canine semen

Strzeżek,

Lecewicz,

Orzeł

et al. 2024

Polish Journal of Veterinary Sciences

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The aim of this study was to evaluate the quality parameters and selected biochemical markers of canine semen sampled at 24-h intervals over a period of 5 days, preceded by 6 months of sexual abstinence. Full ejaculates were obtained from 6 dogs. Ejaculate volume and total sperm counts in the ejaculate decreased gradually on successive sampling days. The percentage of total motile spermatozoa (TMOT), percentage of progressively motile spermatozoa (PMOT), sperm plasma membrane integrity (SPMI), and sperm mitochondrial membrane potential (MMP) increased on successive days of sampling. In addition, ATP content increased in spermatozoa. Total protein content (TPC) and the activity of aspartate aminotransferase (AAT), alkaline phosphatase (AP), and acid phosphatase (AcP) decreased in seminal plasma. Repeated ejaculation over a period of 5 days induced changes in the qualitative and quantitative parameters of canine semen. A decrease in the values of some biochemical markers of semen, secreted by the epididymis and the prostate gland, could point to disturbances in the secretory activity of these organs. Canine semen sampled after prolonged sexual abstinence is generally characterized by less desirable quality parameters, and this observation should be taken into consideration when semen is collected for artificial insemination or preservation. Semen quality can be significantly improved by repeating the sampling procedure after 24 hours. One the other hand, repeated sampling on successive days can significantly decrease total sperm counts in the ejaculate. As a result, a sufficient number of semen doses for artificial insemination may not be obtained from a single ejaculate.

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Isolation and characteristics of aspartate aminotransferase from boar spermatozoa

Cited by 10 publications

References 27 publications

Relationship between biochemical constituents of fish semen and fertility: the effect of short-term storage

Relationship between biochemical constituents of fish semen and fertility: the effect of short-term storage

Boar seminal plasma components and their relation with semen quality

Effect of repeated semen ejaculation on sperm quality and selected biochemical markers of canine semen

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