Isolation and Characteristics of Mesenchymal Stromal Cells from Different Parts of Placenta

Semenova, Ekaterina; Mrowiec, Zbigniew; Machaj, Eugeniusz K; Murzyn, Magdalena; Borg, Katarzyna; Boruczkowski, Dariusz; Otdak, Tomasz

doi:10.4172/2157-7633.1000376

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…MSCs were isolated mechanically as described previously [ 8 ]. The main procedures were as follows: each part was gently separated and washed with sterile saline (0.9% solution of NaCl; FRESENIUS SE&CO. KGaA, Germany) supplemented with antibiotics/antimycotic solution, which contain 10,000 units/mL of penicillin, 10,000 μg/mL of streptomycin, and 25 μg/mL of Gibco Amphotericin B, as recommended for use at 10 mL/L (Antibiotic-Antimycotic (100x) catalog no.…”

Section: Methodsmentioning

confidence: 99%

“…The unique multipotency, immunomodulation capabilities with low or none immunogenicity, due to the low expression of HLA class II proteins define MSC's as potential source of therapeutic agents for regenerative medicine. The immunomodulatory features allow to use them in allo-and autogenic configuration [7,8]. Another important aspect of clinical use is the secretion of various growth factors and cytokines, such as G-CSF, HGF, PDGFAA, TGF-β, IL-6, IL-8 and others, which play important roles not only in immunomodulation but also in promotion of cell proliferation, differentiation, growth and tissue repair [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Mesenchymal Stromal Cells from Different Parts of Umbilical Cord: Approach to Comparison & Characteristics

Semenova

Grudniak

Machaj

et al. 2021

Stem Cell Rev and Rep

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Mesenchymal stromal/stem cells (MSCs) are a unique population of cells that play an important role in the regeneration potential of the body. MSCs exhibit a characteristic phenotype and are capable of modulating the immune response. MSCs can be isolated from various tissues such as: bone marrow, adipose tissue, placenta, umbilical cord and others. The umbilical cord as a source of MSCs, has strong advantages, such as no-risk procedure of tissue retrieval after birth and easiness of the MSCs isolation. As the umbilical cord (UC) is a complex organ and we decided to evaluate, whether the cells derived from different regions of umbilical cord show similar or distinct properties. In this study we characterized and compared MSCs from three regions of the umbilical cord: Wharton’s Jelly (WJ), the perivascular space (PRV) and the umbilical membrane (UCM). The analysis was carried out in terms of morphology, phenotype, immunomodulation potential and secretome. Based on the obtained results, we were able to conclude, that MSCs derived from distinct UC regions differ in their properties. According to our result WJ-MSCs have high and stabile proliferation potential and phenotype, when compare with other MSCs and can be treated as a preferable source of cells for medical application. Graphical abstract

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stromal Cells from Different Parts of Umbilical Cord: Approach to Comparison & Characteristics

Semenova

Grudniak

Machaj

et al. 2021

Stem Cell Rev and Rep

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“…MSCs from Wharton's jelly and placenta were isolated with the same protocol: mechanically and enzymatically, using collagenase IV (Gibco), as previously described [12]. All tissue samples were washed in sterile 0.9% NaCl with antibiotics/antimicotic (Gibco).…”

Section: Cell Isolationmentioning

confidence: 99%

Comparison of Characteristics of Mesenchymal Stem Cells Obtained Mechanically and Enzymatically from Placenta and Umbilical Cord

Semenova¹,

Machaj²,

Ołdak³

2016

J Cell Sci Ther

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Mesenchymal stem cells (MSC) are a good source for the cell therapy thanks to their abilities. MSCs exert very important immunomodulatory effects: they suppress T-and B-cell proliferation and natural killer cell function, and they also limit the expression of the Major Histocompatibility Complex II. Placenta and umbilical cord are the most convenient sources of MSCs, which do not entail ethical problems. Additionally, placental delivery and isolation of umbilical cord is not connected with a difficult or invasive method. MSCs from Wharton's jelly and placenta (the amnion, the chorion, the villi, the Decidua basalis) were isolated with the protocol: mechanically and enzymatically (collagenase digestion). The proliferation potential was evaluated with a PDT analysis. For multi-differentiation of MSCs, cells were incubated in different differentiation media for 2-3 weeks at 37°C in an atmosphere with 5% CO₂ and 90% humidity.We successfully isolated MSCs from placenta and umbilical cord. Mechanical isolation was possible for all types of tissues. In the case of enzymatic digestion of the umbilical cord, we were able to obtain MSCs in a limited number only, insufficient for further analysis.All the isolated MSCs had typical fibroblastic morphology, expressed cell-surface markers (CD73, CD90, CD105), did not express hematopoietic and endothelial markers and differentiated into osteocytes, chondrocytes, and adipocytes, which is a set of possibilities recommended by the International Society of Cell Therapy. We didn't notice significant differences in morphology or differentiation between cells due to tissue of origin and method of isolation. Both: collagenase digestion and mechanical cutting resulted in high amount of cells harvested, but usage of enzyme makes this process faster.

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Development of a novel gene expression panel for the characterization of MSCs for increased biological safety

Różycka-Baczyńska,

Stepaniec,

Warzycha

et al. 2024

J Appl Genetics

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Mesenchymal stromal cells (MSCs) have a wide range of therapeutic applications due to their multipotency, immunomodulatory, and anti-inflammatory properties. Their ability to migrate and recolonize damaged tissues is also remarkable. However, the controversial occurrence of spontaneous tumorigenesis or malignant transformation of MSCs raises concerns about proposed cell-based therapies for patients that researchers must address. There are several in vitro and in vivo strategies for MSC safety approval, but there is still no described coherent scheme that allows the assessment of MSC oncogenic potential in a simple, robust, and reproducible manner. Here, we have developed a diagnostic panel of molecular markers that allows for the accurate verification of the quality and safety of MSCs. Moreover, presented in this article diagnostic panel that can define the origin and tumorigenicity of MSCs can be easily introduced into the routine quality control processes of MSC-based product manufacturing which will improve further clinical applications of MSCs. Graphical Abstract

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Isolation and Characteristics of Mesenchymal Stromal Cells from Different Parts of Placenta

Cited by 3 publications

References 29 publications

Mesenchymal Stromal Cells from Different Parts of Umbilical Cord: Approach to Comparison & Characteristics

Mesenchymal Stromal Cells from Different Parts of Umbilical Cord: Approach to Comparison & Characteristics

Comparison of Characteristics of Mesenchymal Stem Cells Obtained Mechanically and Enzymatically from Placenta and Umbilical Cord

Development of a novel gene expression panel for the characterization of MSCs for increased biological safety

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