Isolation and Characteristics of Primordial Germ Cells in Chicken

Томгорова, Е. К.; Волкова, Н. А.; Волкова, Л. И.; Фисинин, В.И.; Zinovieva, N. A.

doi:10.15389/agrobiology.2012.6.61eng

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…Much of the research was performed on chickens [15][16][17][18][19][20]. The identification and characterization of PGC of other types of agricultural poultry is topical.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Cultivation and Characterization of Quail Primordial Germ Cells

Волкова¹,

Багиров²,

Томгорова³

et al. 2017

S-h. biol.

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A b s t r a c tThe use of avian primordial germ cells (PGCs) for production of chimeric and transgenic poultry is regarded as an alternative way to traditional methods of selection and transgenesis. This approach involves the introduction of donor primordial germ cells in the dorsal aorta of the recipient embryo during their migration from blood to gonads. In case of recipient embryo gonads colonization by donor PGCs, the further differentiation of donor cells into mature germ cells becomes possible, for both males and females. A key factor for the effectiveness of this manipulation is obtaining of a pure population of embryonic cells. In this regard, the development of effective methods for isolation and maintenance of PGCs in culture is important. Our research was aimed at the improvement of methodical approaches for isolation and cultivation of quail PGCs. This type of cells was isolated and characterized. Five-to six-day embryos were used for obtaining PGCs culture. Isolation of PGCs from quail embryos was performed using two methodological approaches -mechanical dissociation and enzymatic treatment. Trypsin solution at concentration from 0.05 % to 0.25 % was used as proteolytic enzyme for enzymatic treatment. In order to obtain the most pure populations of PGCs, unequal ability to adhesion of different types of cells was taken into account. It was found that enzymatic treatment with 0.05 % trypsin is an effective method for embryos disaggregation preserving significant proportion of viability (94 %) of fetal cells. Separation of different cell types based on their different ability to adhesion allows obtaining PGCs culture maximally purified from other cell types. Moreover, single embryo fibroblasts, remaining in the PGCs cell suspension after separation from the other cell types are used as a feeder layer to which PGCs are attached at the subsequent cultivation. It was shown that own primary embryonic fibroblasts are optimal as a feeder layer for short-term PGCs culturing as compared to the use of STO cells and cultured embryonic fibroblasts. If using the growth medium based on DMEM with high glucose level (4.5 g/l) supplemented with 20 % fetal bovine serum, 2 mM glutamine, 10 -6 mM 2-mercaptoethanol, 2 ng/ml LIF (leukemia inhibitory factor), 10 mM essential amino acids (MEM), the antibiotic gentamicin (50 ug/ml) the attachment of PGCs to the feeder layer was observed at day 1 to 2 of cultivation forming colonies at day 3 to 4. The presence of primordial germ cell colonies was confirmed by immunohistochemistry using specific primary antibodies to SSEA-1 (stage-specific embryonic antigen-1).

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“…Much of the research was performed on chickens [15][16][17][18][19][20]. The identification and characterization of PGC of other types of agricultural poultry is topical.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Cultivation and Characterization of Quail Primordial Germ Cells

Волкова¹,

Багиров²,

Томгорова³

et al. 2017

S-h. biol.

Self Cite

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The use of poly additive Nabicat in the feeding of laying hens

Николаев

Andreenko

2020

Kormlenie Sel'skohozjajstvennyh Zhivotnyh I Kormoproizvodstvo (Feeding of Agricultural Animals and Feed Production)

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In modern poultry farming the most urgent tasks are to search for and test new environmentally safe feed and economically justifi ed additives that stimulate productivity, positively aff ect the health of poultry, and consequently increase the livability of livestock and other important zootechnical traits. In order to maintain physiologically normal processes of life, metabolism and increase the level of productivity of poultry it needs mineral components and their chelated compounds. The purpose of this work was to increase the egg productivity of poultry by using the silicon-containing poly additive Nabicat in feeding young hens and laying hens. The researches have been carried out on the poultry cross Highsex Brown under the environments in CJSC “Poultry Farm “Volzhskaya” in the Sredneakhtubinsky area in the Volgograd region. In this paper, The optimal dose of the introduction of the silicon-containing poly additive Nabicat in the diets of young and laying hens of the cross Highsex Brown has been scientifi cally justifi ed and the biological value of using an organic form of silicon has been determined. The positive infl uence of the optimal dose of poly additive on growth, consumption, and digestibility, nutrient metabolism in the poultry body, as well as egg productivity and quality of the resulting egg has been revealed. Thus, the average egg productivity per 1 hen in the control group during the experiment period was 321,3 pcs., and in the 1st, 2nd and 3rd experimental groups it was 322,6 pcs., 325,8 and 325,7 pcs., which was higher than in the control group by 0,40 %, 1,40 and 1,37 %, respectively. The average egg weight in the 1st experimental group exceeded the control by 0,43 g or 0,68 %, in the 2nd experimental group by 1,16 g or 1,84 %, in the 3rd experimental group by 0,81 g or 1,28 %. The economic eff ectiveness of using the organic silicon additive in the composition of Nabicat poly additive in the feeding of laying hens has been proved. The economic eff ect due to the use of diff erent doses of the introduction of silicon-containing poly additive Nabicat in compound feed for laying hens of the 1st experimental group was 141,66 rubles, the 2nd experimental group 962,76 rubles, and the 3rd experimental group 821,89 rubles.

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Isolation and Characteristics of Primordial Germ Cells in Chicken

Cited by 2 publications

References 8 publications

Isolation, Cultivation and Characterization of Quail Primordial Germ Cells

Isolation, Cultivation and Characterization of Quail Primordial Germ Cells

The use of poly additive Nabicat in the feeding of laying hens

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