Isolation and characterization for carcinoembryonic antigen

Coligan, John E.; Lautenschleger, Joel T.; Egan, Marianne L.; Todd, Charles W.

doi:10.1016/0019-2791(72)90308-4

Cited by 106 publications

(22 citation statements)

References 12 publications

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“…Coligan et al (3) isolated CEA from several gastrointestinal tumors and found that their CEA preparation (CEA-City of Hope) was immunologically indistinguishable from CEA prepared by Gold and Freedman (CEA-Montreal) (4). The "First British CEA Standard" (CEA-British) was prepared from liver metastases arising from colon carcinoma by methods similar to those used by Gold and Freedman (4) and Coligan et al (3), and was found (by the National Institute of Biological Standards and Control, London) to be similar to the CEA-Montreal and CEA-City of Hope by immunodiffusion and polyacrylamide gel electrophoresis, as well as in amino-acid and carbohydrate composition.…”

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confidence: 99%

“…Differences between serum and liver CEA CEA standards and antigen preparations used for raising anti-CEA antisera are usually prepared from liver metastases (1)(2)(3)(4)(5). Therefore, we compared the CEA in the serum of this patient to the CEA extracted from his own liver metastases.…”

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“…The "First British CEA Standard" (CEA-British) was prepared from liver metastases arising from colon carcinoma by methods similar to those used by Gold and Freedman (4) and Coligan et al (3), and was found (by the National Institute of Biological Standards and Control, London) to be similar to the CEA-Montreal and CEA-City of Hope by immunodiffusion and polyacrylamide gel electrophoresis, as well as in amino-acid and carbohydrate composition. Hoffmann-LaRoche (Nutley, N.J.) prepared their own CEA (CEA-Roche) and developed a standardized method and kit for routine clinical assays (5,6).…”

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Carcinoembryonic antigen: evidence for multiple antigenic determinants and isoantigens.

Vrba¹,

Alpert²,

Isselbacher³

1975

Proc. Natl. Acad. Sci. U.S.A.

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Carcinoembryonic antigen (CEA) preparations, from various sources were compared by radioimmunoassay. The preparations studied included four CEA standards (CEA-Roche, CEA-Montreal, CEA-City of Hope, and CEA-British) and CEA from serum and liver metastases of a patient with cancer of the colon who had an extremely high concentration of serum CEA (more than 26,000 ng/ml). The data indicate that the CEA-Roche standard differs significantly from the other three CEA standards tested, and that the serum CEA from the patient was antigenically different from currently available CEA standards as well as from the CEA obtained from the patient's own liver metastases. These antigenic differences were reflected in radioimmunoassay inhibition curves that were different and that were not affected by perchloric acid extraction of CEA. Because of the antigenic variation in the serum CEA, markedly different CEA concentrations (varying by three orders of magnitude) were measurable by two different antisera (Roche and Montreal). All the various CEA standards and samples cochromatographed on columns of Sepharose-6B, despite the large antigenic variation.We postulate that CEA consists of a family of "isoantigens" with multiple antigenic determinants. We identified a serum CEA isoantigen that was different from the currently available standards. Consequently, we believe that results of radioimmunoassays currently used for CEA measurement may not represent absolute concentrations of serum "CEA", but may reflect the binding affinity of different isoantigens to a particular polyvalent CEA antiserum.After the demonstration of a tumor-specific antigen (carcinoembryonic antigen, CEA) in adenocarcinomas of the gastrointestinal tract by Gold and Freedman (1), it was shown that immunoreactive CEA was present in the plasma of almost all patients with colon carcinoma. This suggested that serologic measurement of this antigen could be a useful diagnostic test for this malignancy (2). However, the availability of a well characterized CEA standard is an important prerequisite for a valid, generally applicable CEA assay.Coligan et al. (3) isolated CEA from several gastrointestinal tumors and found that their CEA preparation (CEA-City of Hope) was immunologically indistinguishable from CEA prepared by Gold and Freedman (CEA-Montreal) (4). The "First British CEA Standard" (CEA-British) was prepared from liver metastases arising from colon carcinoma by methods similar to those used by Gold and Freedman (4) and Coligan et al. (3), and was found (by the National Institute of Biological Standards and Control, London) to be similar to the CEA-Montreal and CEA-City of Hope by immunodiffusion and polyacrylamide gel electrophoresis, as well as in amino-acid and carbohydrate composition. Hoffmann-LaRoche (Nutley, N.J.) prepared their own CEA (CEA-Roche) and developed a standardized method and kit for routine clinical assays (5, 6). Although studies in which both Roche and Montreal reagents were used showed a high degree of correlation of positive CEA te...

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Carcinoembryonic antigen: evidence for multiple antigenic determinants and isoantigens.

Vrba¹,

Alpert²,

Isselbacher³

1975

Proc. Natl. Acad. Sci. U.S.A.

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“…Carcinoembryonic antigen (CEA) (1) is usually purified from crude tissue hpmogenates of liver metastases pf colorectal carcinomas (2,3). Only few attempts have been made to isölate CEA or CEA related antigens from membrane fractions of colonic carcinomas (4, 5,6).…”

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confidence: 99%

Binding of Carcinoembryonic Antigen (CEA) to an Anti-CEA Immunosorbent in the Presence of Detergents

Wagener¹,

Schwonzen²,

Breuer³

1982

CCLM

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“…The carcinoembryonic antigen (CEA) of the colon is a glycoprotein isolated and defined by Gold and his colleagues in 1965 (1). This protein, characteristic of many kinds of cancer tissues, has a molecular weight of about 200,000 (2,3). It is rather heterogeneous (4) and 50-75% of its content is of a polysaccharide nature (2).…”

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Immunological cross-reactivity of antibodies to a synthetic undecapeptide analogous to the amino terminal segment of carcinoembryonic antigen, with the intact protein and with human sera.

Arnon¹,

Bustin²,

Calef³

et al. 1976

Proc. Natl. Acad. Sci. U.S.A.

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A peptide corresponding to the 11 amino acid residues of the NHT-terminal portion in the sequence of carcinoembryonic antigen(CEA) has been synthesized by the solid phase technique. The synthetic CEA(1-11) peptide was attached by means of a water-soluble carbodiimide reagent to multichain poly(DL-alanine) as well as to bovine serum albumin. Both macromolecular conjugates provoked in rabbit anti-CEA(1-11) peptide antibodies. The specificity of this immunological system and the crossreactivity between the peptide and intact CEA were investigated-by two methods-passive hemagglutination and modified bacteriophage inactivation. Hemagglutination experiments showed that not only anti-CEA(1-11) sera, but also anti-CEA sera, agglutinated CEA(1-11)coated sheep erythrocytes, and both these reactions were inhibited with CEA(1-11) peptide. In experiments with the chemically modified bacteriophage technique CEA(1-11)coated phage was efficiently inactivated with antisera against the CEA(1-11) conjugates, and the inactivation reaction could be totally inhibited with the free peptide. The semipure CEA, but not the pure protein, could also inhibit the phage inactivation, even thoug less efficiently.On the basis of the above results, sera of some cancer patients were tested for their capacity, to inhibit the inactivation of CEA(1-11)coated phage by means of anti-CEA(1-11) antiserum. The results indicate that sera from a large proportion of patients with adenocarcinomas of the digestive tract, pancreas, and breast are capable of inhibiting the above inactivation, whereas most normal sera do not inhibit.

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Isolation and characterization for carcinoembryonic antigen

Cited by 106 publications

References 12 publications

Carcinoembryonic antigen: evidence for multiple antigenic determinants and isoantigens.

Carcinoembryonic antigen: evidence for multiple antigenic determinants and isoantigens.

Binding of Carcinoembryonic Antigen (CEA) to an Anti-CEA Immunosorbent in the Presence of Detergents

Immunological cross-reactivity of antibodies to a synthetic undecapeptide analogous to the amino terminal segment of carcinoembryonic antigen, with the intact protein and with human sera.

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