Isolation and characterization of 21 polymorphic microsatellite markers for a new labeonine fish (Paraqianlabeo lineatus Zhao et al. 2014) using illumina paired-end sequencing

Development of 34 polynucleotide repeat microsatellites for Chinese lake gudgeon (Sarcocheilichthys sinensis Bleeker, 1871) by transcriptome sequencing

“…Deviation of HWE occurred in 12 loci, which might be caused by the existence of null alleles, since 8 of the 12 loci were detected to have probable null alleles (ssE19, ssE25, ssE34, ssE36, ssE37, ssE39, ssE40 and ssE43). Ten loci presented potential null alleles, which might due to the existence of mutations in primer annealing sites (Callen et al., ); similar frequency of null alleles has also been reported in other cyprinids such as Paraqianlabeo lineatus (6 of 21 loci; Li et al., ) and Megalobrama pellegrini (5 of 15 loci; Luo et al., ). In addition, given the declining resource of S. sinensis in the Hongze Lake (Lin et al., ), other factors including non‐random mating, inbreeding and genetic drift may also be causations for null alleles as well as HWE deviation and LD (Hou, Zhang, Wu, Zhang, & Song, ).…”

“…High output sequencing has become an outstanding novel and universal method for the isolation of microsatellite markers in both animals and plants in recent years, because it is low cost and less time‐consuming compared to traditional methods (Li, Zhao, Peng, & Zhang, ; McCracken et al., ). In the present study, a total of 34 novel polymorphic microsatellites were successfully isolated for S. sinensis .…”

Isolation and characterization of ten polymorphic microsatellite loci fromGymnocypris chuiand cross-amplification in seven highly specialized Schizothoracinae fishes

“…As previously shown, microsatellites are valuable tools for analyzing population structure, gene flow and introgression in fishes (Zeng et al, 2013;Boscari et al, 2015;Li et al, 2015;Zhao et al, 2015). In this study, ten novel polymorphic microsatellites were developed for the Tibetan fish, Gymnocypris chui.…”

Nineteen novel microsatellites in Chinese lake gudgeonSarcocheilichthys sinensisBleeker, 1971

“…In this study, we developed 19 polymorphic microsatellites for Chinese lake gudgeon S. sinensis . Until recently, only the mitochondrial genome was sequenced for this species (Li, Zhao, Peng, & Zhang, ; Zhou, Zhang, Wang, & Zheng, ), however there had thus far been no nuclear markers available. Our microsatellites can help to evaluate the population genetic structure of the S. sinensis, to supply valuable information for conservation management.…”