Isolation and characterization of 48 polymorphic microsatellite markers for the blood clam Scapharca broughtonii (Arcidae)

Abstract

ABSTRACT. Blood clams (Scapharca broughtonii) are widely cultivated and consumed in noutheast Asia. Forty-eight polymorphic microsatellite loci were developed for this clam using magnetic-bead hybridization enrichment. The number of alleles per locus ranged from 2 to 14. Polymorphism of these loci was assessed in 30 individuals from a population collected from coastal areas of Qingdao, China. The values of observed heterozygosity, expected heterozygosity and polymorphism information content per locus ranged from 0.1034 to 0.9655, from 0.1831 to 0.9208, and from 0.1638 to 0.8964, respectively. Forty-three of 48 loci conformed to Hardy-Weinberg equilibrium. These microsatellite loci would be useful for molecular 4502 J.-T. Tian et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (4): 4501-4507 (2012) genetic breeding, population genetics, genome mapping, and other relevant research on S. broughtonii.

Effects of Acute Low-Salinity Stress on the Activities of Catalase (CAT), Superoxide Dismutase (SOD) and Glutathiones-Transferase (GST) in Scapharca broughtonii

“…The ark shell, Scapharca broughtonii, is one of the most commercially important shellfish distributed in China, Korea and Japan, and has become a popularly cultured species in North China due to its high economic value in recent years (Xia et al, 2015;Zheng et al, 2015). However, the wild yield and resource of S. broughtonii have been suffering to severe population decline because of over-exploitation, disease outbreak, and the deterioration of environmental conditions over the past few decades (Li et al, 2013;Tian et al, 2012). Therefore, it is very difficult to meet market demand because of insufficient wild yield, which promoted the development of cultured S. broughtonii in China (Bai et al, 2016;Hong et al, 2002).…”