Isolation and characterization of a metal-reducing Pseudomonas sp. strain 135 with amide-degrading capability

Yakasai, Mohd Hafeez; Rahman, Mohd Fadhil; Rahim, Mohd. Badrin Hanizam Abd; Khayat, Mohd Ezuan; Shamaan, Nor Aripin; Shukor, Mohd Yunus

doi:10.54987/bstr.v5i2.361

Cited by 9 publications

(4 citation statements)

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“…A concentration of copper at 1.0 mg/L leads to a complete cessation of reduction. This was also observed in many Mo-reduction works [32][33][34][35][36][37][38][39][40][41].…”

Section: Resultssupporting

confidence: 72%

Modelling the Effect of Copper on the Mo-reduction Rate of the Antarctic Bacterium Pseudomonas sp. strain DRY1

Rahman¹,

Ahmad²,

MacCormack³

et al. 2017

J Environ Microbiol Toxicol

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Molybdenum reduction by the Antarctic bacterium Pseudomonas sp. strain DRY1 is strongly inhibited by copper. Mo reduction by this bacterium at 10 mM sodium molybdate shows a sigmoidal pattern with lag periods ranging from 7 to 10 h at various concentrations of copper. As the concentration of copper was increased, the overall Mo reduction rate was inhibited with 1.2 mg/L causing the cessation of Mo reduction rate. The modified Gompertz model was utilized to obtain Mo reduction rates at different concentrations of copper. The Mo reduction rates obtained from the modified Gompertz model was then modelled according to the modified Han-Levenspiel, Wang, Liu, modified Andrews and the Amor models. Out of the five models, only Wang, modified Han-Levenspiel and the Liu models were able to fit the curve, whilst the modified Andrews and Amor models were unable to fit the curves. Both the Wang and modified Han-Levenspiel models show acceptable fitting while the Liu model shows poor fitting. Results of the statistical analysis showed that the modified Han-Levenspiel model was the best model based on the lowest values for RMSE and AICc, highest adjusted correlation coefficient (adR2) and values of AF and BF closest to unity. The parameters obtained from the modified Han Levenspiel model, which were Ccrit, ïmax and m which represent critical heavy metal ion concentration (mg/l), maximum reduction rate (nmole Mo blue/h) and empirical constant values were 0.225 (95%, confidence interval from 0.198 to 0.251), 1.200 (95%, confidence interval from 1.180 to 1.220) and 0.443 (95%, confidence interval from 0.261 to 0.626). The modified Han-Levenspiel accurately predicted the critical copper concentration that completely inhibited molybdenum reduction rate in this bacterium.

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“…A concentration of copper at 1.0 mg/L leads to a complete cessation of reduction. This was also observed in many Mo-reduction works [32][33][34][35][36][37][38][39][40][41].…”

Section: Resultssupporting

confidence: 72%

Modelling the Effect of Copper on the Mo-reduction Rate of the Antarctic Bacterium Pseudomonas sp. strain DRY1

Rahman¹,

Ahmad²,

MacCormack³

et al. 2017

J Environ Microbiol Toxicol

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“…The bacterium was previously isolated as a Mo-reducer [24]. From an overnight pure culture of the bacterium grown in 100 mL of nutrient broth, 0.1 mL was added into 45 mL of acrylamide enrichment medium in a 100 mL volumetric flask and the culture was incubated at 150 rpm for 48 h at 25 ℃ on an incubator shaker (Certomat R, USA).…”

Section: Growth and Maintenance Of Acrylamide-degrading Bacteriummentioning

confidence: 99%

Characterization of the Growth of Pseudomonas sp. strain DrY135 on Acrylamide

Rahman¹,

Khayat²,

Yakasai³

et al. 2022

J Environ Microbiol Toxicol

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This study investigated the growth properties of a molybdenum-reducing bacteria previously isolated for its ability to break down amides. The bacterial growth range is 500–1000 mg/L, 6.5–8.0 pH, and 30–35 °C. The presence of hazardous heavy metals such as mercury, silver, and copper impeded this bacterium's development on acrylamide. The protracted lag phase seen when growing on acrylamide demonstrates the compound's severe growth inhibition. This bacterium has the potential to be an effective acrylamide bioremediation agent due to its greater tolerance for acrylamide than other acrylamide-degrading bacteria identified in the scientific literature. The influence of initial pH on bacterial growth at room temperature indicates that the optimal pH range lies between 6.5 and 8.0. The ideal temperature range for plant growth was between 30 and 35 oC. In a series of experiments utilizing a starting concentration of 1% (w/v) of various organic carbon sources, it was determined that glucose supported the most cellular growth on acrylamide, followed by sucrose, fructose, mannose, and citrate, in descending order of efficiency, whereas mannitol did not support growth. Doses of 300 and 500 mg/L of acrylamide stimulated the most rapid growth expansion, but concentrations of 1500 mg/L and above completely halted development. Copper (Cu), lead (Pb), cadmium (Cd), chromium (Cr), and mercury (Hg) were investigated at a concentration of 2 ppm. Mercury hindered growth by 71 percent, copper by 72 percent, and cadmium by 52 percent, according to our findings. There was a linear association between the acrylamide content and the delay before this bacterium began to develop. A lag time of one to three days was found when the acrylamide content grew from 100 to 1,500 mg/L. As quantities of acrylamide increased, so did the maximal growth rate, indicating an overall pattern of increasing toxicity.

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“…The bacterium was previously isolated as a Mo-reducer that demonstrates amides-degrading capability [34]. To characterize the acrylamide degradation capability of this bacterium, an overnight pure culture of the bacterium was grown in 1 L of nutrient broth, centrifuged at 10,000 ×g for 10 min and the pellet resuspended in sterile tap water thrice and the pellet dissolved in sterile tap water to and OD600 nm of 1.0.…”

Section: Growth and Maintenance Of Acrylamide-degrading Bacteriummentioning

confidence: 99%

Response Surface Method for the Optimization of Pseudomonas sp. strain DrY135 Growth on Acrylamide as a Nitrogen Source

Uba

Manogaran²,

Yakasai

et al. 2022

Bull Environ Sci Sust Manage

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Acrylamide pollution and contamination can come from a variety of sources and is an emerging toxic pollutant that need remediation. A molybdenum-reducing bacteria that had been previously isolated and demonstrated the ability to degrade amides was further studied of its critical parameters contributing to optimum growth on acrylamide. The Box-Behnken design was utilized in optimizing the three previously identified significant components (pH, incubation time and acrylamide concentration). ANOVA, the pertubation's plot, and several other diagnostic plots were utilized in the analysis of the significant factors or parameters that contributed. The model was supported by the diagnostic plots including the half-normal, Cook's distance, leverage vs runs, residual vs runs, Box-Cox, DFFITS, and DFBETAS. Conditions that were predicted to be optimal were found and analyzed in order to find the optimal growth given the factors that were used and to determine the optimal concentration, which was 1 g/L. The solutions for finding the optimal growth predicted a growth maximum of 12.055 Log CFU/mL (95 percent confidence interval (C.I.) from 11.550 to 12.593), and verification using experimental results resulted in a growth of 12.908 Log CFU/mL (12.744 to 13.072) with the results being close to the predicted values but was significantly higher than the predicted data. The second numerical optimization was for predicting the highest acrylamide concentration tolerable for growth and the solution shows a predicted a growth maximum of 12.055 Log CFU/mL (95 percent confidence interval from 11.550 to 12.593). This solution was experimentally verified resulting in a growth of 12.195 Log CFU/mL (95 percent confidence interval from 11.806 to 12.584) with the actual results being in accordance with the predicted values. The results of the RSM exercise showed that growth on acrylamide may be optimized more effectively with RSM than with OFAT, which indicates that RSM is more useful than OFAT in this regard.

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Isolation and characterization of a metal-reducing Pseudomonas sp. strain 135 with amide-degrading capability

Cited by 9 publications

References 0 publications

Modelling the Effect of Copper on the Mo-reduction Rate of the Antarctic Bacterium Pseudomonas sp. strain DRY1

Modelling the Effect of Copper on the Mo-reduction Rate of the Antarctic Bacterium Pseudomonas sp. strain DRY1

Characterization of the Growth of Pseudomonas sp. strain DrY135 on Acrylamide

Response Surface Method for the Optimization of Pseudomonas sp. strain DrY135 Growth on Acrylamide as a Nitrogen Source

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