Isolation and characterization of a prokaryotic sulfurtransferase.

Aird, Bruce A.; Heinrikson, R L; Westley, John

doi:10.1016/s0021-9258(18)45381-1

Cited by 18 publications

(3 citation statements)

References 23 publications

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“…In addition to the phsABC system, 14 Zodletone genomes belonging to 6 phyla encoded a rhodanase-like enzyme (EC 2.8.1.1 or EC 2.8.1.3) for thiosulfate disproportionation, as well as enzymes for both sulfite oxidation (by means of reversal of sulfate reduction via Sat+AprAB or the sulfite dehydrogenase SoeABC), and sulfite reduction (via the dissimilatory sulfite reductases Dsr or Asr), where the final products of thiosulfate disproportionation are expected to be both hydrogen sulfide and sulfate ( 30 – 34 ) ( Fig. 4 , Fig.…”

Section: Resultsmentioning

confidence: 99%

Microbial Diversity and Sulfur Cycling in an Early Earth Analogue: From Ancient Novelty to Modern Commonality

Hahn

Farag

Murphy

et al. 2022

mBio

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Section: Resultsmentioning

confidence: 99%

Microbial Diversity and Sulfur Cycling in an Early Earth Analogue: From Ancient Novelty to Modern Commonality

Hahn

Farag

Murphy

et al. 2022

mBio

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“…Genes for a truncated Sox pathway are also present (tSox) (Welte et al, 2009) oxidizing thiosulphate to elemental sulphur and to sulphite, in the periplasm. The presence of putative sulphane sulphurtransferase genes (Tst; newly reported here) (Aird et al, 1987) can indicate thiosulphate disproportionation to sulphite and sulphide in the periplasm. A gene encoding for the recently characterized thiosulphate uptake protein YeeE (Tanaka et al, 2020) was also identified in the genome.…”

Section: Number Of Contigsmentioning

confidence: 78%

The complete and closed genome of the facultative generalist Candidatus Endoriftia persephone from deep‐sea hydrothermal vents

Oliveira

Srivastava

Espada-Hinojosa

et al. 2022

Molecular Ecology Resources

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The mutualistic interactions between Riftia pachyptila and its endosymbiont Candidatus Endoriftia persephone (short Endoriftia) have been extensively researched. However, the closed Endoriftia genome is still lacking. Here, by employing single‐molecule real‐time sequencing we present the closed chromosomal sequence of Endoriftia. In contrast to theoretical predictions of enlarged and mobile genetic element‐rich genomes related to facultative endosymbionts, the closed Endoriftia genome is streamlined with fewer than expected coding sequence regions, insertion‐, prophage‐sequences and transposase‐coding sequences. Automated and manually curated functional analyses indicated that Endoriftia is more versatile regarding sulphur metabolism than previously reported. We identified the presence of two identical rRNA operons and two long CRISPR regions in the closed genome. Additionally, pangenome analyses revealed the presence of three types of secretion systems (II, IV and VI) in the different Endoriftia populations indicating lineage‐specific adaptations. The in depth mobilome characterization identified the presence of shared genomic islands in the different Endoriftia drafts and in the closed genome, suggesting that the acquisition of foreign DNA predates the geographical dispersal of the different endosymbiont populations. Finally, we found no evidence of epigenetic regulation in Endoriftia, as revealed by gene screenings and absence of methylated modified base motifs in the genome. As a matter of fact, the restriction‐modification system seems to be dysfunctional in Endoriftia, pointing to a higher importance of molecular memory‐based immunity against phages via spacer incorporation into CRISPR system. The Endoriftia genome is the first closed tubeworm endosymbiont to date and will be valuable for future gene oriented and evolutionary comparative studies.

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“…Second, in pure culture members of the family Enterobacteriaceae (Gammaproteobacteria), thiosulfate disproportionation is known to occur via the quinone-dependent membrane-bound molybdopterin-containing thiosulfate reductase PhsABC 75 . Finally, thiosulfate disproportionation to sul te and hydrogen sul de can also occur via a rhodanase-like enzyme [EC: 2.8.1.1 or EC: 2.8.1.3], as shown for several bacterial lineages [76][77][78][79][80] , although this could be part of a thiosulfate assimilatory pathway as recently shown in E. coli 81 .…”

Section: Mainmentioning

confidence: 99%

Microbial diversity in an early earth analogue: From ancient novelty to modern commonality

Hahn¹,

Farag²,

Murphy³

et al. 2021

Preprint

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Life emerged and diversified in the absence of molecular oxygen 1. The prevailing anoxia and unique sulfur chemistry in the Paleo-, Meso- and Neoarchean, and early Proterozoic eons may have supported microbial communities that are drastically different than those currently thriving on the earth’s surface 2–4. Zodletone spring in southwestern Oklahoma represents a unique habitat where spatial sampling could substitute for geological eons: from the anoxic, surficial light-exposed sediments simulating a preoxygenated earth, to overlaid water column where air exposure simulates the relentless oxygen intrusion during the Neo-Proterozoic 5. We discovered a remarkably diverse microbial community in the spring sediments, with two thirds (340/516) of the metagenomic assembled genomes belonging to 200 bacterial and archaeal families that were either previously undescribed or are extremely rare elsewhere on earth. Such diversity is underpinned by the widespread occurrence of sulfite-, thiosulfate-, tetrathionate-, and sulfur-reduction, in contrast with a paucity of sulfate-reduction metabolism in those taxa. This greatly expands the diversity of lineages mediating reductive sulfur cycling processes in the tree of life. In the overlaying water community oxygen intrusion leads to the establishment of a significantly less diverse community dominated by well-characterized lineages and the prevalence of oxidative sulfur cycling processes. Such transition from ancient novelty to modern commonality underscores the profound impact of the great oxygenation event on the earth’s surficial anoxic community.. It also suggests that novel and rare lineages encountered in current anaerobic habitats could represent taxa once thriving on the anoxic earth that have failed to adapt to the progressive oxygenation.

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Isolation and characterization of a prokaryotic sulfurtransferase.

Cited by 18 publications

References 23 publications

Microbial Diversity and Sulfur Cycling in an Early Earth Analogue: From Ancient Novelty to Modern Commonality

Microbial Diversity and Sulfur Cycling in an Early Earth Analogue: From Ancient Novelty to Modern Commonality

The complete and closed genome of the facultative generalist Candidatus Endoriftia persephone from deep‐sea hydrothermal vents

Microbial diversity in an early earth analogue: From ancient novelty to modern commonality

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