Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720

Onek, L. A.; Lea, Peter J.; Smith, Roger

doi:10.1007/s002030050066

Cited by 5 publications

(6 citation statements)

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“…Collectively, these studies indicate that Ca 2+ plays dual roles in regulating ROS homeostasis. SDS‐PAGE profile also revealed prominent bands of approximately 70, 33, 21, and 14 kDa, which have molecular weight very near to the aalmodulin/calmodulin like protein . However, their presence could be confirmed only through immunoblotting or mass spectrometry.…”

Section: Discussionmentioning

confidence: 99%

Unraveling of cross talk between Ca²⁺ and ROS regulating enzymes in Anabaena 7120 and ntcA mutant

Singh

Mishra

2015

J. Basic Microbiol.

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In order to understand a cross talk between Ca(2+) and ROS regulating enzymes and the possible involvement of ntcA gene, Anabaena sp. PCC 7120 and its derivative ntcA mutant grown in varied levels of calcium chloride (0, 1, 10, and 100 mM) have been investigated. Scanning Electron Microscopy showed abnormal structure formation at high calcium concentration (100 mM) both in wild type and mutant. Fv /Fm values suggested that 100 mM calcium concentration was detrimental for photosynthetic apparatus. SOD, catalase, APX, GR, and peroxidase activity were found to be maximum for 100 mM and minimum for 1 mM of exogenously supplied calcium salt. NADPH contents were higher for wild type than mutant. RAPD-PCR and SDS-PAGE analysis revealed a difference in DNA as well as proteome pattern with changes in calcium chloride regime. Prominent bands of approximately 70, 33, 21, and 14 kDa expressed in the wild type served as the marker polypeptide bands under calcium supplementation. Results suggest that higher levels of calcium ion disturb the cellular homeostasis generating ROS, thereby inducing enhanced levels of antioxidative enzymes. Further, data also suggests possible involvement of ntcA gene in cross talk between calcium ion and ROS regulating enzymes.

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Section: Discussionmentioning

confidence: 99%

Unraveling of cross talk between Ca²⁺ and ROS regulating enzymes in Anabaena 7120 and ntcA mutant

Singh

Mishra

2015

J. Basic Microbiol.

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“…Nevertheless, the possible presence of CaM in chloroplasts is intriguing from an evolutionary perspective. Whereas CaM is considered a typical eukaryotic protein, others suggest that prokaryotes do possess CaM‐like proteins (Pettersson & Bergman, 1989; Onek & Smith, 1992; Onek et al. , 1994; Smith, 1996).…”

Section: Cam and Plant Responses To Environmental Stimulimentioning

confidence: 99%

Calmodulin as a versatile calcium signal transducer in plants

2001

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SummaryThe complexity of Ca 2 + patterns observed in eukaryotic cells, including plants, has led to the hypothesis that specific patterns of Ca 2 + propagation, termed Ca 2 + signatures, encode information and relay it to downstream elements (effectors) for translation into appropriate cellular responses. Ca 2 + -binding proteins (sensors) play a key role in decoding Ca 2 + signatures and transducing signals by activating specific targets and pathways. Calmodulin is a Ca 2 + sensor known to modulate the activity of many mammalian proteins, whose targets in plants are now being actively characterized. Plants possess an interesting and rapidly growing list of calmodulin targets with a variety of cellular roles. Nevertheless, many targets appear to be unique to plants and remain uncharacterized, calling for a concerted effort to elucidate their functions. Moreover, the extended family of calmodulin-related proteins in plants consists of evolutionarily divergent members, mostly of unknown function, although some have recently been implicated in stress responses. It is hoped that advances in functional genomics, and the research tools it generates, will help to explain the multiplicity of calmodulin genes in plants, and to identify their downstream effectors. This review summarizes current knowledge of the Ca 2 + -calmodulin messenger system in plants and presents suggestions for future areas of research. AbbreviationsCaM, clamodulin; Ca 2 + /CaM, Ca 2 + -bound CaM; CaMBD, CaM-binding domain; CCaMK, chimeric Ca 2 + /CaM-dependent protein kinase; CDPK, calcium-depentent (calmodulin-independent) protein kinase; CNGC, cyclic nucleotide gated channel; cNMP, cyclic nucleotide monophosphate; KCBP, kinesin-like CaM-binding protein.© New Phytologist (2001) 151 : 35 -66

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“…More over, some of these proteins were found to contain two or several structural fragments resembling the EF hand motif [56,57]. Currently, several bacterial CaM like proteins have been characterized and the correspond ing genes have been cloned [55][56][57][58][59][60]. A large group of CaM like proteins was described in Streptomyceta ceae, although only a few of them contained four EF hand motifs [57].…”

Section: Calcium Intake By Bacterial Cellsmentioning

confidence: 99%

Ca2+ signaling in prokaryotes

Шемарова

Нестеров²

2014

Microbiology

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431In both higher and lower eukaryotes, Ca 2+ ions are common intracellular signaling messengers. Along with cyclic nucleotides, diacylglycerol, nitric oxide, signaling oligopeptides, and some other active mole cules and ions, Ca 2+ cations are involved in the regula tion of numerous processes in eukaryotic cells, such as cell cycle, nuclear division, nerve impulse conduction, muscle contraction, and others [1][2][3].In prokaryotes, the role of Ca 2+ in intracellular sig naling is considerably less studied. Only a few works have considered Ca 2+ ions as potential regulators of such cellular processes as ion transport, spore germi nation, heterocyst differentiation, gene expression, cell motility, adaptation to low or high temperatures, virulence, etc. [5][6][7][8][9][10]. For a long time, investigation of Ca 2+ signaling in microbial cells was hindered by methodological problems in determining intracellular calcium concentrations ([Ca 2+ ] i ), calcium contami nation, reagent toxicity, and difficulties in handling individual cells; these questions were discussed in [11][12][13]. However, the unique optical methods and other approaches to investigation of individual bacte rial cells developed in the 1990s, which made it possi ble to register the changes in Ca 2+ levels in microbial cytosol in response to various external stimuli [14][15][16], boosted the studies of the regulatory functions of Ca 2+ in prokaryotic signaling.In the present review, we discuss the historical development of ideas concerning Ca 2+ signaling in prokaryotic cells and the current concepts of the related research in evolutionary biology. FUNCTIONS OF Ca 2+ IN PROKARYTIC CELLSThe first studies concerning the role of Ca 2+ as a secondary messenger in prokaryotic organisms date back to the 1970s, when Ordal found that decreased Ca 2+ intake by bacterial cells affected the chemotactic behavior in Bacillus subtilis [17]. Further research confirmed his observations in other microorganisms. A study on Escherichia coli showed that a decrease in [Ca 2+ ] i could alter the swimming mode in flagellate bacteria. Specifically, the characteristic slow and directed swimming changed to chaotic disordered motion [18]. Measurements of [Ca 2+ ] i levels in E. coli performed using the Fura 2 fluorescent Ca 2+ indicator revealed that bacterial repellents caused a temporary increase in [Ca 2+ ] i (to over 100 nM) and inhibited chemotaxis, whereas attractants, in contrast, tempo rarily decreased [Ca 2+ ] i levels and stimulated chemot axis [18].Experiments on blocking the voltage gated Ca 2+ channels (Cav) of different types (L, P/Q, and R) showed that inhibitors added to the incubation medium impaired the chemotactic behavior of bacte ria. In prokaryotes, it was expected that the stimuli inducing chemotactic reactions may increase the intake of extracellular Ca 2+ through the ion channels opened in response to membrane depolarization, as it occurs in protists and even in multicellular eukaryotes. However, there was no direct correlation between the extracellular...

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Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720

Cited by 5 publications

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Unraveling of cross talk between Ca²⁺ and ROS regulating enzymes in Anabaena 7120 and ntcA mutant

Unraveling of cross talk between Ca²⁺ and ROS regulating enzymes in Anabaena 7120 and ntcA mutant

Calmodulin as a versatile calcium signal transducer in plants

Ca2+ signaling in prokaryotes

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Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720

Cited by 5 publications

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Unraveling of cross talk between Ca2+ and ROS regulating enzymes in Anabaena 7120 and ntcA mutant

Unraveling of cross talk between Ca2+ and ROS regulating enzymes in Anabaena 7120 and ntcA mutant

Calmodulin as a versatile calcium signal transducer in plants

Ca2+ signaling in prokaryotes

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Unraveling of cross talk between Ca²⁺ and ROS regulating enzymes in Anabaena 7120 and ntcA mutant

Unraveling of cross talk between Ca²⁺ and ROS regulating enzymes in Anabaena 7120 and ntcA mutant