Isolation and characterization of a bacterial strain Hydrogenophaga sp. PYR1 for anaerobic pyrene and benzo[a]pyrene biodegradation

Yan, Zaisheng; Zhang, Yu; Wu, Haixia; Yang, Mingzhong; Zhang, Haichen; Hao, Zheng; Jiang, Helong

doi:10.1039/c7ra09274a

Cited by 75 publications

(33 citation statements)

References 45 publications

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“…BAP5 [ 43 ], Ochrobactrum sp. VA1 [ 25 ], Hydrogenophaga PYR1 [ 44 ], Cellulosimicrobium cellulans CWS2 [ 45 ], Rhizobium tropici CIAT 899 [ 46 ], Klebsiella pneumonia PL1 [ 47 ] and Pseudomonas sp. JP1 [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Degradation of benzo[a]pyrene by halophilic bacterial strain Staphylococcus haemoliticus strain 10SBZ1A

et al. 2021

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The exploitation of petroleum oil generates a considerable amount of “produced water or petroleum waste effluent (PWE)” that is contaminated with polycyclic aromatic hydrocarbons (PAHs), including Benzo[a]pyrene (BaP). PWE is characterised by its high salinity, which can be as high as 30% NaCl, thus the exploitation of biodegradation to remove PAHs necessitates the use of active halophilic microbes. The strain 10SBZ1A was isolated from oil contaminated soils, by enrichment experiment in medium containing 10% NaCl (w/v). Homology analyses of 16S rRNA sequences identified 10SBZ1A as a Staphylococcus haemoliticus species, based on 99.99% homology (NCBI, accession number GI: MN388897). The strain could grow in the presence of 4–200 μmol l-1 of BaP as the sole source of carbon, with a doubling time of 17–42 h. This strain optimum conditions for growth were 37 oC, 10% NaCl (w/v) and pH 7, and under these conditions, it degraded BaP at a rate of 0.8 μmol l-1 per day. The strain 10SBZ1A actively degraded PAHs of lower molecular weights than that of BaP, including pyrene, phenanthrene, anthracene. This strain was also capable of removing 80% of BaP in the context of soil spiked with BaP (10 μmol l-1 in 100 g of soil) within 30 days. Finally, a metabolic pathway of BaP was proposed, based on the identified metabolites using liquid chromatography-high resolution tandem mass spectrometry. To the best of our knowledge, this is the first report of a halophilic BaP degrading bacterial strain at salinity > 5% NaCl.

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Section: Discussionmentioning

confidence: 99%

Degradation of benzo[a]pyrene by halophilic bacterial strain Staphylococcus haemoliticus strain 10SBZ1A

et al. 2021

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“…Hydrogenophaga sp. isolated from polycyclic aromatic hydrocarbon-contaminated soil can degrade pyrene under iron-reducing anaerobic conditions (Yan et al 2017). Rhodoferax ferrireducens isolated from subsurface sediments showed the activity as microbial fuel cells for electricity generation (Risso et al 2009).…”

Section: Isolation and Identification Of Methylene Blue Decolorizing mentioning

confidence: 99%

Methylene blue decolorizing bacteria isolated from water sewage in Yogyakarta, Indonesia

et al. 2020

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Abstract. Michelle, Siregar RAN, Sanjaya A, Jap L, Pinontoan R. 2020. Methylene blue decolorizing bacteria isolated from water sewage in Yogyakarta, Indonesia. Biodiversitas 21: 1136-1141. The textile industry contributes to water pollution issues all over the world. One of the most commonly applied cationic dye in the textile industry is methylene blue. This study aimed to isolate bacteria with the potential to decolorize methylene blue from dye contaminated sewage water located in Kulon Progo District, Yogyakarta, where several textile industries within the proximity, are located. Characterizations of bacterial candidates were done morphologically and biochemically. Molecular identification was conducted by 16S rRNA sequencing. The ability of isolates to decolorize methylene blue was observed by the reduction of methylene blue’s maximum absorption at the wavelength of 665 nm. The results showed that isolates were identified as Comamonas aquatica and Ralstonia mannitolilytica. C. aquatica PMB-1 and R. mannitolilytica PMB-2 isolates were able to decolorize methylene blue with decolorization percentage of 67.9% and 60.3%, respectively when incubated for 96 hours at 37°C. These findings present information on the capability of the genus Ralstonia and Comamonas to decolorize methylene blue cationic dye.

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“…Exceptions comprise Hydrogenophaga intermedia S1 and PMC, which mineralize 4-aminobenzenesulfonate in two-species consortia (Gan et al, 2011) and the pyrene-degrading Hydrogenophaga sp. PYR1 (Yan et al, 2017). The third new PromA plasmid is plasmid pEN1, which was obtained by biparental exogenous isolation from a BPS operating on a farm near Kortrijk, Belgium (Dealtry et al, 2016), selecting for mercury resistant transconjugants of the recipient strain.…”

Section: Introductionmentioning

confidence: 99%

PromA Plasmids Are Instrumental in the Dissemination of Linuron Catabolic Genes Between Different Genera

et al. 2020

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PromA plasmids are broad host range (BHR) plasmids, which are often cryptic and hence have an uncertain ecological role. We present three novel PromA γ plasmids which carry genes associated with degradation of the phenylurea herbicide linuron, two of which originated from unrelated Hydrogenophaga hosts isolated from different environments (pPBL-H3-2 and pBPS33-2), and one (pEN1) which was exogenously captured from an on-farm biopurification system (BPS). Hydrogenophaga sp. plasmid pBPS33-2 carries all three necessary gene clusters (hylA, dca, ccd) determining the three main steps for conversion of linuron to Krebs cycle intermediates, while pEN1 only determines the initial linuron hydrolysis step. Hydrogenophaga sp. plasmid pPBL-H3-2 exists as two variants, both containing ccd but with the hylA and dca gene modules interchanged between each other at exactly the same location. Linuron catabolic gene clusters that determine the same step were identical on all plasmids, encompassed in differently arranged constellations and characterized by the presence of multiple IS1071 elements. In all plasmids except pEN1, the insertion spot of the catabolic genes in the PromA γ plasmids was the same. Highly similar PromA plasmids carrying the linuron degrading gene cargo at the same insertion spot were previously identified in linuron degrading Variovorax sp. Interestingly, in both Hydrogenophaga populations not every PromA plasmid copy carries catabolic genes. The results indicate that PromA plasmids are important vehicles of linuron catabolic gene dissemination, rather than being cryptic and only important for the mobilization of other plasmids.

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Isolation and characterization of a bacterial strain Hydrogenophaga sp. PYR1 for anaerobic pyrene and benzo[a]pyrene biodegradation

Cited by 75 publications

References 45 publications

Degradation of benzo[a]pyrene by halophilic bacterial strain Staphylococcus haemoliticus strain 10SBZ1A

Degradation of benzo[a]pyrene by halophilic bacterial strain Staphylococcus haemoliticus strain 10SBZ1A

Methylene blue decolorizing bacteria isolated from water sewage in Yogyakarta, Indonesia

PromA Plasmids Are Instrumental in the Dissemination of Linuron Catabolic Genes Between Different Genera

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