Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi

Kang, Shaozhu; Zhang, Luhao; Liao, Jiaming; Zhang, Dongzhuo; Wu, Shao-Yi; Zhang, Xin; Hu, Weihui; Wei, Jingguang

doi:10.3390/v14081802

Cited by 8 publications

(8 citation statements)

References 63 publications

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“…Furthermore, greater than 50% of R18L phage remained active after incubation at 4-40°C for 24-48 h. The stability of R18L below 40°C simplifies the storage and transport requirements for this phage. Most phages become inactive when the temperature reaches 55°C, with survival percentages lower than 15% within 3 h. R18L did not show any activity when the temperature increased to 60°C, showing better thermal tolerance than previously reported vibriophages IME271 (40°C) (Li et al, 2019) and V-YDF132 (Kang et al, 2022). R18L exhibited stability over a wide range of pH (pH 6-11) for 3 h (Figure 5B).…”

Section: Virion Stability Of R18lmentioning

confidence: 66%

“…As shown in the TEM micrograph, R18L has an icosahedral capsid (diameter 88.6 ± 2.2 nm) and a long noncontractile tail (225 ± 2.2 nm in length and 11 ± 1.0 nm in width), being a siphovirus-like phage (Figure 1B). Different vibriophages have been isolated and characterized using V. harveyi as the host, with most belonging to siphoviruses (Vinod et al, 2006;Wang et al, 2017;Wu et al, 2021;Droubogiannis and Katharios, 2022;Kang et al, 2022), followed by myoviuses (Surekhamol et al, 2014;Lal et al, 2017) and podoviruses (Thiyagarajan et al, 2011). The reason why siphoviruses are the predominant viral group infecting Vibrio is currently unknown.…”

Section: Biological Features Of R18lmentioning

confidence: 99%

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A broad-host-range lytic phage vB_VhaS-R18L as a candidate against vibriosis

Cai

Tian

et al. 2023

Front. Microbiol.

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Vibriosis is one of the most common bacterial diseases that cause high rates of mortality and considerable economic losses in aquaculture. Phage therapy has been considered as a promising alternative method to antibiotics in the biocontrol of infectious diseases. Genome sequencing and characterization of the phage candidates are prerequisites before field applications to ensure environmental safety. In this study, a lytic phage, named vB_VhaS-R18L (R18L), was isolated from the coastal seawater of Dongshan Island, China. The phage was characterized in terms of morphology, genetic content, infection kinetics, lytic profile, and virion stability. Transmission electronic microscopy indicated that R18L is siphovirus-like, comprising an icosahedral head (diameter 88.6 ± 2.2 nm) and a long noncontractile tail (225 × 11 nm). Genome analysis indicated R18L to be a double-stranded DNA virus with a genome size of 80,965 bp and a G + C content of 44.96%. No genes that encode known toxins or genes implicated in lysogeny control were found in R18L. A one-step growth experiment showed that R18L had a latent period of approximately 40 min and a burst size of 54 phage particles per infected cell. R18L showed lytic activity against a wide range of at least five Vibrio species (V. alginolyticus, V. cholerae, V. harveyi, V. parahemolyticus, and V. proteolyticus). R18L was relatively stable at pH 6–11 and at temperatures ranging from 4°C to 50°C. The broad lytic activity across Vibrio species and the stability in the environment make R18L a potential candidate for phage therapy in controlling vibriosis in aquaculture systems.

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Section: Virion Stability Of R18lmentioning

confidence: 66%

Section: Biological Features Of R18lmentioning

confidence: 99%

A broad-host-range lytic phage vB_VhaS-R18L as a candidate against vibriosis

Cai

Tian

et al. 2023

Front. Microbiol.

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“…Additionally, phage BUCT549 exhibited a latent period of approximately 30-40 min and an average burst size of 141 pfu/cell [46]. In another example, phage V-YDF132 displayed a latent period of 20 min and a burst size of 298 pfu/cell [47]. Conversely, vB_VcaS_HC had a notably longer latent period of 1.5 h, but showcased a larger burst size of 172 pfu per infected cell [48].…”

Section: Discussionmentioning

confidence: 96%

Lysin and Lytic Phages Reduce Vibrio Counts in Live Feed and Fish Larvae

Romero,

Blas-Chumacero,

Urzúa

et al. 2024

Microorganisms

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Vibrio species are naturally found in estuarine and marine ecosystems, but are also recognized as significant human enteropathogens, often linked to seafood-related illnesses. In aquaculture settings, Vibrio poses a substantial risk of infectious diseases, resulting in considerable stock losses and prompting the use of antimicrobials. However, this practice contributes to the proliferation of antimicrobial-resistant (AMR) bacteria and resistance genes. Our investigation aimed to explore the potential of biological agents such as bacteriophage CH20 and endolysin LysVPp1 in reducing Vibrio bacterial loads in both rotifer and fish larvae. LysVPp1’s lytic activity was assessed by measuring absorbance reduction against various pathogenic Vibrio strains. Phage CH20 exhibited a limited host range, affecting only Vibrio alginolyticus GV09, a highly pathogenic strain. Both CH20 and LysVPp1 were evaluated for their effectiveness in reducing Vibrio load in rotifers or fish larvae through short-setting bioassays. Our results demonstrated the significant lytic effect of endolysin LysVPp1 on strains of Vibrio alginolyticus, Vibrio parahaemolyticus, and Vibrio splendidus. Furthermore, we have showcased the feasibility of reducing the load of pathogenic Vibrio in live feed and fish larvae by using a non-antibiotic-based approach, such as lytic phage and endolysin LysVPp1, thus contributing to the progress of a sustainable aquaculture from a One Health perspective.

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“…Additionally, R21Y maintained high lytic activity over varying environmental conditions (4-55 • C and pH 4-10). R21Y showed better thermal tolerance than vB_VhaM_pir03 (45 • C) [21], VPMCC5 (45 • C) [50], vB_VhaS_MAG7 (45 • C) [54], V-YDF132 (50 • C) [49], and vB_VhaS-R18L (50 • C) [53] and greater acid tolerance than V-YDF132 (pH 5-11) and vB_VhaS-R18L (pH 6-11). Thus, R21Y can remain stable and exert bactericidal activity in practical applications, suggesting that it is a promising candidate for biological control of V. harveyi disease.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Vibriophage Infecting Pathogenic Vibrio harveyi

Li,

Yun,

Chen

et al. 2023

IJMS

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Bacterial diseases caused by Vibrio spp. are prevalent in aquaculture and can lead to high mortality rates among aquatic species and significant economic losses. With the increasing emergence of multidrug-resistant Vibrio strains, phage therapy is being explored as a potential alternative to antibiotics for biocontrol of infectious diseases. Here, a new lytic phage named vB_VhaS_R21Y (R21Y) was isolated against Vibrio harveyi BVH1 obtained from seawater from a scallop-farming area in Rongcheng, China. Its morphology, infection cycle, lytic profile, phage stability, and genetic features were characterized. Transmission electronic microscopy indicated that R21Y is siphovirus-like, comprising an icosahedral head (diameter 73.31 ± 2.09 nm) and long noncontractile tail (205.55 ± 0.75 nm). In a one-step growth experiment, R21Y had a 40-min latent period and a burst size of 35 phage particles per infected cell. R21Y was highly species-specific in the host range test and was relatively stable at pH 4–10 and 4–55 °C. Genomic analysis showed that R21Y is a double-stranded DNA virus with a genome size of 82,795 bp and GC content of 47.48%. Its high tolerance and lytic activity indicated that R21Y may be a candidate for phage therapy in controlling vibriosis in aquacultural systems.

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Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi

Cited by 8 publications

References 63 publications

A broad-host-range lytic phage vB_VhaS-R18L as a candidate against vibriosis

A broad-host-range lytic phage vB_VhaS-R18L as a candidate against vibriosis

Lysin and Lytic Phages Reduce Vibrio Counts in Live Feed and Fish Larvae

Characterization of a Vibriophage Infecting Pathogenic Vibrio harveyi

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