Isolation and characterization of a Cr(VI)-reduction Ochrobactrum sp. strain CSCr-3 from chromium landfill

He, Zhiguo; Gao, Fengling; Tao, Shuxia; Hu, Yuehua; He, Chao

doi:10.1016/j.jhazmat.2008.07.041

Cited by 137 publications

(93 citation statements)

References 24 publications

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“…The same trend has been described by several other authors such as He et al [22] which showed optimal chromium reduction and Ochrobactrum sp. (strain CSCr-3) growth in an initial pH of 10.…”

Section: Phsupporting

confidence: 88%

Hexavalent Chromium Reduction and Accumulation by Acinetobacter AB1 Isolated from Fez Tanneries in Morocco

et al. 2011

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Hexavalent chromium reduction and accumulation by Acinetobacter AB1 isolated from Fez tanneries effluents were tested. The effects of some environmental factors such as pH, temperature, and exposure time on Cr(VI) reduction and resistance were investigated. We found that this strain was able to resist to concentrations as high as 400 mg/l of Cr(VI). Moreover, pH 10 and the temperature 30°C constitute favourable conditions to the growth and reduction of Acinetobacter AB1. Complete reduction of Cr(VI) was observed at low initial Cr(VI) concentrations of 50 mg/l after 72 h of incubation. Furthermore, Transmission electron microscope (TEM) analysis showed morphological changes in AB1 strain due 48H exposure to 100 mg/l chromate concentration and revealed circular electron dense (dark black point) inclusion within the cell cytoplasm suggesting chromium deposition within the cells.

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Section: Phsupporting

confidence: 88%

Hexavalent Chromium Reduction and Accumulation by Acinetobacter AB1 Isolated from Fez Tanneries in Morocco

et al. 2011

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“…SUK 1205 [26], Ochrobactrum sp. strain CSCr-3 [24], and Bacillus cereus [32] were also found to utilize glucose as electron donor for efficient chromate reduction.…”

Section: Effect Of Different Electron Donorsmentioning

confidence: 99%

“…ES 29 [7], O. intermedium strain SDCr-5 [30], Ochrobactrum sp. strain CSCr-3 [24], Amphibacillus sp. KSUCr3, Bacillus sp.…”

Section: Effect Of Metal Ionsmentioning

confidence: 99%

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Reduction of Hexavalent Chromium by Viable Cells of Chromium Resistant Bacteria Isolated from Chromite Mining Environment

2014

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Environmental contamination of hexavalent chromium [Cr(VI)] is of serious concern for its toxicity as well as mutagenic and carcinogenic effects. Bacterial chromate reduction is a cost-effective technology for detoxification as well as removal of Cr(VI) from polluted environment. Chromium resistant and reducing bacteria, belonging to Arthrobacter, Pseudomonas, and Corynebacterium isolated from chromite mine overburden and seepage samples of Orissa, India, were found to tolerate 12-18 mM Cr(VI) during growth. Viable cells of these isolates were also capable of growing and reducing 100 M Cr(VI) quite efficiently in Vogel Bonner (V.B.) broth under batch cultivation. Freshly grown cells of the most potent isolate, Arthrobacter SUK 1201, reduced 100 M Cr(VI) in 48 h. Reduction potential of SUK 1201 cells decreased with increase in Cr(VI) concentration but increased with increase in cell density and attained its maximum at 10 10 cells/mL. Chromate reducing efficiency of SUK 1201 was promoted in the presence of glucose and glycerol while the highest reduction was at pH 7.0 and 25 ∘ C. The reduction process was inhibited by divalent cations Ni, Co, and Cd, but not by Cu. Similarly, carbonyl cyanide m-chlorophenylhydrazone, N,N,-Di cyclohexyl carbodiimide, sodium azide, and sodium fluoride were inhibitory to chromate reduction, while 2,4 dinitrophenol promoted the process. Cells permeabilized by toluene increased the efficiency of Cr(VI) reduction and, thereby, indicate that Arthrobacter sp. SUK 1201, indigenous to chromite mining environment, could be used as an ideal tool for chromium bioremediation.

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“…Characterization of chromate reduction by a flavin reductase from E. coli revealed faster reduction when compared to chemical reduction by NADH and glutathione suggesting that unlike eukaryotes, enzymatic reduction is the dominant mechanism in bacterial cells (Puzon et al 2002). Chromate reductase encoding genes vary according to the microorganism and also occurs in He et al (2009) plasmids, few literatures are available describing the gene sequence (Barak et al 2006;Gonzalez et al 2005). A soluble Cr(VI) reductase purified from the cytoplasm of E. coli ATCC 33456 was a dimmer with a molecular mass of 84 kDa.…”

Section: Purification and Characterization Of Bacterial Chromate Redumentioning

confidence: 99%

Remediation of chromium contaminants using bacteria

Kanmani¹,

Aravind²,

Preston

2011

Int. J. Environ. Sci. Technol.

125

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Chromium contaminants emanating from industrial activities pose a significant threat to human's wellbeing. Chromium (III) and Chromium (VI) are the forms in which they are commonly encountered, of which the trivalent form is relatively benign. Hence, biological reduction of hexavalent chromium has been widely explored by researchers, yielding fruitful outcomes, opening up exciting avenues and also throwing up new challenges. This article attempts to review this area of research. Microbes, especially bacteria capable of Chromium (VI) reduction, belonging to a heterogeneous group have been isolated from contaminated sites. They exhibit plasmid-mediated chromate resistance and the reduction is enzymatically mediated. Reduction studies have been carried out with free and immobilized enzymes as well as whole cells. Experiments have been carried out in specifically designed bioreactors operated in batch and continuous modes. Although significant progress has been made, much needs to be done for its successful in situ application as the organism may not withstand the Chromium concentration or may be impeded by the presence of other toxicants. With molecular engineering, it may be possible to derive strains with improved performance even under stressful field conditions.

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Isolation and characterization of a Cr(VI)-reduction Ochrobactrum sp. strain CSCr-3 from chromium landfill

Cited by 137 publications

References 24 publications

Hexavalent Chromium Reduction and Accumulation by Acinetobacter AB1 Isolated from Fez Tanneries in Morocco

Hexavalent Chromium Reduction and Accumulation by Acinetobacter AB1 Isolated from Fez Tanneries in Morocco

Reduction of Hexavalent Chromium by Viable Cells of Chromium Resistant Bacteria Isolated from Chromite Mining Environment

Remediation of chromium contaminants using bacteria

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