Isolation and characterization of a cellulolytic actinomycete Microbispora bispora

Waldron, Clive; Becker-Vallone, C. A.; Eveleigh, D. E.

doi:10.1007/bf00250327

Cited by 41 publications

(24 citation statements)

References 21 publications

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“…Although Waldron et al (56) isolated Microbispora from several soil samples, streams, pools, and compost samples, there are no other reports of Microbispora inhabiting compost. The high population of Microbispora -related isolates identified in both compost types examined here suggests that these strains are well adapted to composting conditions.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Characterization of Cellulose-decomposing Bacteria Inhabiting Sawdust and Coffee Residue Composts

et al. 2012

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Clarifying the identity and enzymatic activities of microorganisms associated with the decomposition of organic materials is expected to contribute to the evaluation and improvement of composting processes. In this study, we examined the cellulolytic and hemicellulolytic abilities of bacteria isolated from sawdust compost (SDC) and coffee residue compost (CRC). Cellulolytic bacteria were isolated using Dubos mineral salt agar containing azurine cross-linked (AZCL) HE-cellulose. Bacterial identification was performed based on the sequence analysis of 16S rRNA genes, and cellulase, xylanase, β-glucanase, mannanase, and protease activities were characterized using insoluble AZCL-linked substrates. Eleven isolates were obtained from SDC and 10 isolates from CRC. DNA analysis indicated that the isolates from SDC and CRC belonged to the genera Streptomyces, Microbispora, and Paenibacillus, and the genera Streptomyces, Microbispora, and Cohnella, respectively. Microbispora was the most dominant genus in both compost types. All isolates, with the exception of two isolates lacking mannanase activity, showed cellulase, xylanase, β-glucanase, and mannanase activities. Based on enzyme activities expressed as the ratio of hydrolysis zone diameter to colony diameter, it was suggested that the species of Microbispora (SDCB8, SDCB9) and Paenibacillus (SDCB10, SDCB11) in SDC and Microbispora (CRCB2, CRCB6) and Cohnella (CRCB9, CRCB10) in CRC contribute to efficient cellulolytic and hemicellulolytic processes during composting.

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Section: Discussionmentioning

confidence: 99%

“…Waldron et al (56) demonstrated that Microbispora produces thermally stable extracellular cellulase in good yield, while the poor xylanase activity of Microbispora sp. isolated from cattle manure was reported by Holtz et al (21).…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Cellulose-decomposing Bacteria Inhabiting Sawdust and Coffee Residue Composts

et al. 2012

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“…We isolated such a strain from a New Jersey compost while screening for microbes that produce thermostable cellulases (Waldron et al 1986). M. bispora produces endoglucanases, cellobiohydrolases and flglucosidases in its cellulase complex, their syntheses being inducible (Yablonsky et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Cloning of a Microbispora bispora cellobiohydrolase gene in Streptomyces lividans

ChaseJr.

Eveleigh

1993

Appl Microbiol Biotechnol

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The cellobiohydrolase II (CBHII) of Microbispora bispora, originally cloned in Escherichia coli, was subcloned into Streptomyces lividans using shuttle vectors pSKN 01 and pSKN 02. The enzyme was secreted from Streptomyces, whereas it was intracellular in E. coli. The yields of CBHII produced by S. fividans transformants were 15-20-fold higher than those produced by E. coli transformants. The optimal pH of M. bispora native cellobiohydrolase and the cloned enzyme from S. lividans is 6.5. The thermal and pH stability of CHBII produced in M. bispora, E. coli and S. lividans were compared. Enzyme produced in E. coli was inactivated more rapidly (k = 0.252 rain -1 at 90 ° C; 90% inactivation after 10rain vs. 0.119rain -1 for the others). CBHII was monitored following electrophoretic separation by reaction with a monoclonal antibody. The apparent molecular mass of the protein produced from the S. lividans clone was 93 kDa, the same as that of the native enzyme, but that of the enzyme produced in E. coil was smaller (82 kDa).

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“…Microbispora bispora is a thermophilic actinomycete that produces cellulases as effective, but more thermostable, than those of fungi such as Trichoderma reesei (Waldron et al 1986).…”

Section: Introductionmentioning

confidence: 99%

Studies on the extracellular p-nitrophenyl ?-d-cellobiosidase activity of a thermophilic streptomycete

Ball

Brabban

McCarthy

1992

Appl Microbiol Biotechnol

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The activity of ten actinomycete strains against p-nitrophenyl fl-D-cellobioside (pNPC) was investigated. Intra-and extracellular activities were detected in all strains, although activities were found to vary between strains. Extracellular pNPC activity, detected in the thermophilic Streptomycete EC22 was six times greater than that of the next best strain. Culture supernatant studies on Streptomyces strain EC22 revealed that pNPC activity was optimal at 65 ° C and between pH 6.0 and pH 8.0, although this temperature value represented a compromise between increased reaction rate and thermal denaturation. At 65°C, the half-life of activity against pNPC was 150 min. The Km value of pNPC activity was found to be 3.33 mM. Enzyme activity was subject to competitive inhibition by both glucose and cellobiose with substrate inhibition constant (Ki) values of 2.2 mM and 1.4 mM respectively. Fast protein liquid chromatograph (FPLC) of culture supernatants from EC22 using anion exchange chromatography revealed the presence of two proteins with pNPC activity. This result was confirmed using gel-filtration chromatography which estimated the molecular mass of the two proteins to be approximately 48 and 33 kDa, with the specific activity of the 48 kDa enzyme being eight times greater than that of the 33 kDa protein.

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Isolation and characterization of a cellulolytic actinomycete Microbispora bispora

Cited by 41 publications

References 21 publications

Isolation and Characterization of Cellulose-decomposing Bacteria Inhabiting Sawdust and Coffee Residue Composts

Isolation and Characterization of Cellulose-decomposing Bacteria Inhabiting Sawdust and Coffee Residue Composts

Cloning of a Microbispora bispora cellobiohydrolase gene in Streptomyces lividans

Studies on the extracellular p-nitrophenyl ?-d-cellobiosidase activity of a thermophilic streptomycete

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