Isolation and characterization of a crude oil degrading bacteria from formation water: comparative genomic analysis of environmental Ochrobactrum intermedium isolate versus clinical strains

Abstract

Abstract:In this study, we isolated an environmental clone of Ochrobactrum intermedium, strain 2745-2, from the formation water of Changqing oilfield in Shanxi, China, which can degrade crude oil. Strain 2745-2 is aerobic and rod-shaped with optimum growth at 42 °C and pH 5.5. We sequenced the genome and found a single chromosome of 4 800 175 bp, with a G+C content of 57.63%. Sixty RNAs and 4737 protein-coding genes were identified: many of the genes are responsible for the degradation, emulsification, and metabolizing of crude oil. A comparative genomic analysis with related clinical strains (M86, 229E, and LMG3301 T ) showed that genes involved in virulence, disease, defense, phages, prophages, transposable elements, plasmids, and antibiotic resistance are also present in strain 2745-2.

Lipase and biosurfactant from Ochrobactrum intermedium strain MZV101 isolated by washing powder for detergent application

“…Although all isolated bacteria are not capable to become commercially available [ 32 ]. So, screening of new lipase and biosurfactant producing bacteria are still required [ 33 ].…”

“…Ochrobactrum is a gram-negative, strictly aerobic, catalase and oxidase positive and usually single-cell [ 36 ]. O. intermedium 2745–2 was the first environmental strain isolated from formation water in China [ 33 ]. The optimum growth temperature for O. intermedium strains are 20 to 37 °C and optimum are pH 7–7.3 [ 37 ].…”

Taxonomic Organization of the Family Brucellaceae Based on a Phylogenomic Approach

“…The most comprehensive analysis of Brucellaceae species to date, that included more than one hundred strains, showed that the use of these single markers results in different tree topologies depending on the locus and the phylogenetic reconstruction algorithm, and with low to very low branching support throughout the topology (Scholz et al, 2008a). Such lack of resolution was confirmed by a number of other studies which each reported a unique tree topology using the same markers (Teyssier et al, 2007;Huber et al, 2009;Kämpfer et al, 2013Chai et al, 2015;Gazolla Volpiano et al, 2019;Krzyżanowska et al, 2019). Nonetheless, a striking common observation of these various studies is that the genus Brucella was consistently embedded within the Ochrobactrum genus diversity, although no definitive conclusion about the taxonomic status of Brucella spp.…”

“…Clade 2 includes Ochrobactrum species gallinifaecis, thiophenivorans, pseudorignonense, quorumnoscens, rhizospherae, grignonense, and pituitosum. These species consistently group together in most 16S rRNA-based phylogenetic analyses, but usually as an internal Ochrobactrum clade and with O. gallinifaecis excluded (Chai et al, 2015;Gazolla Volpiano et al, 2019;Krzyżanowska et al, 2019). On the contrary, recA and multilocus phylogenies usually separated correctly this group from the other Ochrobactrum spp.…”

Petroleum Degradation: Promising Biotechnological Tools for Bioremediation

“…However, microorganisms have also been enriched and isolated by using methods of various modifications. For instance, oil-degrading bacteria were isolated using sterile crude oil as the medium [9]. …”