Isolation and Characterization of a Bacteriocin-Like Substance Produced by Geobacillus toebii Strain HBB-247

Özdemir, Gamze Başbülbül; Bıyık, Halil

doi:10.1007/s12088-011-0227-x

Cited by 11 publications

(5 citation statements)

References 24 publications

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“…However, the genomes of these strains have not been sequenced and therefore the identity of potential bacteriocin candidates cannot be determined through bioinformatics. This is also the case for G. toebii HBB-247, that has been shown to produce a bacteriocin with an estimated mass of 38 kDa (Başbülbül Özdemir and Biyik, 2012 ; Özdemir and Biyik, 2012 ). There are a number of other bacteriocins of undetermined mass which have been characterized within Geobacillus prior to modern sequencing or mass spectrometry methods (Shafia, 1966 ; Yule and Barridge, 1976 ; Sharp et al, 1979 ; Fikes et al, 1983 ).…”

Section: Discussionmentioning

confidence: 83%

In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus

et al. 2018

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The thermophilic, endospore-forming genus of Geobacillus has historically been associated with spoilage of canned food. However, in recent years it has become the subject of much attention due its biotechnological potential in areas such as enzyme and biofuel applications. One aspect of this genus that has not been fully explored or realized is its use as a source of novel forms of the ribosomally synthesized antimicrobial peptides known as bacteriocins. To date only two bacteriocins have been fully characterized within this genus, i.e., Geobacillin I and II, with only a small number of others partially characterized. Here we bioinformatically investigate the potential of this genus as a source of novel bacteriocins through the use of the in silico screening software BAGEL3, which scans publically available genomes for potential bacteriocin gene clusters. In this study we examined the association of bacteriocin gene presence with niche and phylogenetic position within the genus. We also identified a number of candidates from multiple bacteriocin classes which may be promising antimicrobial candidates when investigated in vitro in future studies.

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Section: Discussionmentioning

confidence: 83%

In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus

et al. 2018

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“…Non-treated CB exhibited an inhibition halo as large as 19.00 mm, while the treated one completely lost (100.0%) his activity (results not shown) after treatment with all proteolytic enzymes. Whereas the treatment with trypsin, pepsin and papain confirms the proteinaceous nature of BLIS, that with α-amylase suggests a complex structure 74 with a saccharide moiety playing an important role in biological activity 75 . In addition, since there are only few examples of circular bacteriocins or BLIS resistant to proteolysis [76][77][78] , these results taken together indicate that BLIS produced by Pediococcus pentosaceus LBM 18 may be a linear proteinaceous glycoactive compound.…”

Section: Sensitivity Of Blis To Hydrolytic Enzymesmentioning

confidence: 90%

Antibacterial and antifungal activity of crude and freeze-dried bacteriocin-like inhibitory substance produced by Pediococcus pentosaceus

Azevedo

Mendonça

Moreno

et al. 2020

Sci Rep

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Pediococcus pentosaceus LBM 18 has shown potential as producer of an antibacterial and antifungal bacteriocin-like inhibitory substance (BLIS). BLIS inhibited the growth of spoilage bacteria belonging to Lactobacillus, Enterococcus and Listeria genera with higher activity than Nisaplin used as control. It gave rise to inhibition halos with diameters from 9.70 to 20.00 mm, with Lactobacillus sakei being the most sensitive strain (13.50-20.00 mm). It also effectively suppressed the growth of fungi isolated from corn grain silage for up to 25 days and impaired morphology of colonies by likely affecting fungal membranes. these results point out that P. pentosaceus BLIS may be used as a new promising alternative to conventional antibacterial and antifungal substances, with potential applications in agriculture and food industry as a natural bio-controlling agent. Moreover, cytotoxicity and cell death induction tests demonstrated cytotoxicity and toxicity of BLiS to human colon adenocarcinoma caco-2cells but not to peripheral blood mononuclear cells, with suggests possible applications of BLIS also in medical-pharmaceutical applications. Lactic acid bacteria (LAB) are a group of well-distributed microorganisms in nature 1-3 having lactic acid as the major product of sugar fermentation 4,5. They are Gram-positive, non-pathogenic, non-sporulating, facultative anaerobic, catalase negative and acid tolerant bacteria with a strictly fermentative metabolism 3,6 , which are often used as industrial starter cultures in food fermentation technology 7,8. Their metabolic products are recognized as Generally Regarded as Safe (GRAS) by the Food and Drug Administration (FDA) and recommended by the Qualified Presumption of Safety (QPS) list of the European Food Safety Authority (EFSA) 9. This means that they can be safely used to preserve foods 10,11 , hence offering important benefits to the food industry 12. From the technology point of view, the most important genera of LAB are Aerococcus, Carnobacterium,

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“…HBB-229, Clostridium pasteurianum (DSM 525) and Cellulomonas fimi (DSM 20114). Its activity against C. pasteurianum (DSM 525) was as high as against G. stearothermophilus (DSMZ 22), which adds a point for classifying this antimicrobial agent as a BLIS rather than bacteriocin [ 60 ].…”

Section: Antimicrobial Potential Of Geobacillus An...mentioning

confidence: 99%

Antimicrobial Potential of the Genera Geobacillus and Parageobacillus, as Well as Endolysins Biosynthesized by Their Bacteriophages

et al. 2022

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In the recent decades, antibiotic resistance has emerged and spread rapidly among clinically relevant pathogens. The natural ability of bacteria to transmit resistance determinants through horizontal gene transfer poses constant challenges to drug development. Natural molecules produced by soil microorganisms continue to be a key source of new antimicrobial agents. In this context, bacteria from the Geobacillus and Parageobacillus genera deserve special attention. Although there is commercial and industrial interest in these microorganisms, the full range of antibacterial compounds biosynthesized by the Geobacillus and Parageobacillus species remains largely unexplored. The aim of this review is to present the strong antimicrobial potential of these bacteria and endolysins produced by their bacteriophages.

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Isolation and Characterization of a Bacteriocin-Like Substance Produced by Geobacillus toebii Strain HBB-247

Cited by 11 publications

References 24 publications

In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus

In silico Prediction and Exploration of Potential Bacteriocin Gene Clusters Within the Bacterial Genus Geobacillus

Antibacterial and antifungal activity of crude and freeze-dried bacteriocin-like inhibitory substance produced by Pediococcus pentosaceus

Antimicrobial Potential of the Genera Geobacillus and Parageobacillus, as Well as Endolysins Biosynthesized by Their Bacteriophages

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