Isolation and characterization of a high H2-producing strain Klebsiella oxytoca HP1 from a hot spring

Long, Minnan; Jin-li, Huang; Xiaobin, Wen; Xu, Hui; Jinzao, C.; Chuannan, L.; Fengzhang, Zhang; Xu, Lei

doi:10.1016/j.resmic.2004.08.004

Cited by 92 publications

(20 citation statements)

References 16 publications

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“…Microaerobic species, that require O 2 partial pressures below atmospheric levels for aerobic metabolism, have been described such as Klebisalle oxytoca HP1. 24 The question of oxygen tolerance and potential microaerobic metabolism of T. neapolitana and T. maritima has been raised in several studies 10,12,14,22,25,26 and is addressed here. Belkin et al 10 and Childers et al 22 found that it was possible to maintain cultures under aerobic conditions at temperatures below the temperature range for growth.…”

Section: V C 2009 American Institute Of Chemical Engineersmentioning

confidence: 99%

The fermentation stoichiometry of Thermotoga neapolitana and influence of temperature, oxygen, and pH on hydrogen production

Munro

Zinder

Walker

2009

Biotechnology Progress

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The hyperthermophilic bacterium, Thermotoga neapolitana, has potential for use in biological hydrogen (H(2)) production. The objectives of this study were to (1) determine the fermentation stoichiometry of Thermotoga neapolitana and examine H(2) production at various growth temperatures, (2) investigate the effect of oxygen (O(2)) on H(2) production, and (3) determine the cause of glucose consumption inhibition. Batch fermentation experiments were conducted at temperatures of 60, 65, 70, 77, and 85 degrees C to determine product yield coefficients and volumetric productivity rates. Yield coefficients did not show significant changes with respect to growth temperature and the rate of H(2) production reached maximum levels in both the 77 degrees C and 85 degrees C experiments. The fermentation stoichiometry for T. neapolitana at 85 degrees C was 3.8 mol H(2), 2 mol CO(2), 1.8 mol acetate, and 0.1 mol lactate produced per mol of glucose consumed. Under microaerobic conditions H(2) production did not increase when compared to anaerobic conditions, which supports other evidence in the literature that T. neapolitana does not produce H(2) through microaerobic metabolism. Glucose consumption was inhibited by a decrease in pH. When pH was adjusted with buffer addition cultures completely consumed available glucose.

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Section: V C 2009 American Institute Of Chemical Engineersmentioning

confidence: 99%

The fermentation stoichiometry of Thermotoga neapolitana and influence of temperature, oxygen, and pH on hydrogen production

Munro

Zinder

Walker

2009

Biotechnology Progress

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“…Indeed, the main goal of the current research on biohydrogen production by fermentation is to increase H 2 yield. Many studies have been conducted to evaluate the attributes of various substrates fermented by different species of bacteria (Chen et al, 2001;Fang et al, 2002;Minnan et al, 2005). Variables such as temperature, pH, HRT, and SRT have been studied to determine their effect on production rate (Collet et al, 2004;Lynd et al, 1989;Pavlostathis et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Continuous hydrogen production during fermentation of α‐cellulose by the thermophillic bacterium Clostridium thermocellum

Magnusson

Çiçek

Sparling

2008

Biotech & Bioengineering

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Continuous hydrogen (H2) production during fermentation of alpha-cellulose was established using the thermophillic, anaerobic bacterium Clostridium thermocellum ATCC 27405. The objectives of this work were to characterize growth of C. thermocellum, quantify H2 production and determine soluble end-product synthesis patterns during fermentation of a cellulosic substrate under continuous culture conditions. A 5 L working volume fermentor was established and growth experiments were maintained for over 3,000 h. Substrate concentrations were varied from 1 to 4 g/L and the feed was introduced with continuous nitrogen gas sparging to prevent clogging of the feed-line. The pH and temperature of the reactor were maintained at 7.0 and 600 degrees C, respectively, throughout the study. At concentrations above 4 g/L, the delivery of alpha-cellulose was impaired due to feed-line clogging and it became difficult to maintain a homogenous suspension. The highest total gas (H2 plus CO2) production rate, 56.6 mL L(-1) h(-1), was observed at a dilution rate of 0.042 h(-1) and substrate concentration of 4 g/L. Under these conditions, the H2 production rate was 5.06 mmol h(-1). Acetate and ethanol were the major soluble end-products, while lactate and formate were greatly reduced compared to production in batch cultures. Concentrations of all metabolites increased with increasing substrate concentration, with the exception of lactate. Despite a number of short-term electrical and mechanical failures during the testing period, the system recovered quickly, exhibiting substantial robustness. A carbon balance was completed to ensure that all end-products were accounted for, with final results indicating near 100% carbon recovery. This study shows that long-term, stable H2 production can be achieved during direct fermentation of an insoluble cellulosic substrate under continuous culture conditions.

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“…Lactic acid bacteria such as Lactobacillus sp. had been reported to decrease hydrogen content in biogas production due to its inhibitory effect caused by the excreted bacteriocins which have an adverse effect on hydrogenproducing bacteria [29]. The microbial community of 89SR3-2 (lane F) was composed of C. acetobutylicum while that the 112YL1 (lane G) consisted of C. beijerinckii and C. acetobutylicum which was found as the active hydrogen producers in the hydrogen fermentation of sugar [27].…”

Section: Microbial Community Analysismentioning

confidence: 98%

Potential for using enriched cultures and thermotolerant bacterial isolates for production of biohydrogen from oil palm sap and microbial community analysis

Noparat

Prasertsan

O‐Thong

2012

International Journal of Hydrogen Energy

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Isolation and characterization of a high H2-producing strain Klebsiella oxytoca HP1 from a hot spring

Cited by 92 publications

References 16 publications

The fermentation stoichiometry of Thermotoga neapolitana and influence of temperature, oxygen, and pH on hydrogen production

The fermentation stoichiometry of Thermotoga neapolitana and influence of temperature, oxygen, and pH on hydrogen production

Continuous hydrogen production during fermentation of α‐cellulose by the thermophillic bacterium Clostridium thermocellum

Potential for using enriched cultures and thermotolerant bacterial isolates for production of biohydrogen from oil palm sap and microbial community analysis

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