Isolation and characterization of a dehydration responsive element binding factor MsDREBA5 in Malus sieversii Roem.

Zhao, Xiangjuan; Lei, Hengjiu; Zhao, Kai; Yuan, Hao; Li, Tianhong

doi:10.1016/j.scienta.2012.05.020

Cited by 15 publications

(11 citation statements)

References 33 publications

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“…In this study, we identified a banana DREB gene, MaDEAR1 , whose predicted protein possesses an APETALA2 (AP2) domain that binds to DREs and an EAR motif that is responsible for transcriptional repression ( Figures 1A and 2 ), and thus belongs to A-5 group ( Figure 1B ). Sub-cellular localization and transcriptional activation assays indicated that MaDEAR1 is nuclear-localized and possesses transcriptional repression activity ( Figure 2 ), similar to GhDBP1 in cotton ( Huang and Liu, 2006 ) and MsDREBA5 in Malus sieversii Roem ( Zhao et al, 2012 ).…”

Section: Discussionmentioning

confidence: 84%

The Banana Transcriptional Repressor MaDEAR1 Negatively Regulates Cell Wall-Modifying Genes Involved in Fruit Ripening

Fan

Kuang

et al. 2016

Front. Plant Sci.

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Ethylene plays an essential role in many biological processes including fruit ripening via modulation of ethylene signaling pathway. Ethylene Response Factors (ERFs) are key transcription factors (TFs) involved in ethylene perception and are divided into AP2, RAV, ERF, and DREB sub-families. Although a number of studies have implicated the involvement of DREB sub-family genes in stress responses, little is known about their roles in fruit ripening. In this study, we identified a DREB TF with a EAR motif, designated as MaDEAR1, which is a nucleus-localized transcriptional repressor. Expression analysis indicated that MaDEAR1 expression was repressed by ethylene, with reduced levels of histone H3 and H4 acetylation at its regulatory regions during fruit ripening. In addition, MaDEAR1 promoter activity was also suppressed in response to ethylene treatment. More importantly, MaDEAR1 directly binds to the DRE/CRT motifs in promoters of several cell wall-modifying genes including MaEXP1/3, MaPG1, MaXTH10, MaPL3, and MaPME3 associated with fruit softening during ripening and represses their activities. These data suggest that MaDEAR1 acts as a transcriptional repressor of cell wall-modifying genes, and may be negatively involved in ethylene-mediated ripening of banana fruit. Our findings provide new insights into the involvement of DREB TFs in the regulation of fruit ripening.

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Section: Discussionmentioning

confidence: 84%

The Banana Transcriptional Repressor MaDEAR1 Negatively Regulates Cell Wall-Modifying Genes Involved in Fruit Ripening

Fan

Kuang

et al. 2016

Front. Plant Sci.

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“…Although whole‐genome sequencing has facilitated the characterization of DREB TFs in apple, only a few DREB genes have been identified and studied to date (Yang et al ., ; Zhao et al ., ,b, ). In this study, we identified 10 DREB6‐type TFs (MsDREB6s) from M. sieversii .…”

Section: Discussionmentioning

confidence: 97%

“…Previously, we identified several TF genes that function in the response to drought stress in M. sieversii (Xu et al ., ), one of the most drought‐tolerant apple rootstocks in China (Li, ), using suppression subtractive hybridization (SSH) analysis. The expression patterns of two of the DREB genes identified in our study, MsDREBA5 and MsDREB2C , and their roles in stress tolerance were further examined (Zhao et al ., , ). In this study, we identified 10 putative DREB6 genes from M. sieversii and examined their expression patterns in response to drought stress.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of MsDREB6.2 results in cytokinin‐deficient developmental phenotypes and enhances drought tolerance in transgenic apple plants

et al. 2017

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Dehydration-responsive element binding factors (DREBs) play important roles in plant growth, development, and stress signaling pathways in model plants. However, little is known about the function of DREBs in apple (Malus × domestica), a widely cultivated crop that is frequently threatened by drought. We isolated a DREB gene from Malus sieversii (Ledeb.) Roem., MsDREB6.2, and investigated its functions using overexpression analysis and chimeric repressor gene-silencing technology (CRES-T). We identified possible target genes of the protein encoded by MsDREB6.2 using electrophoretic mobility shift assays (EMSAs) and chromatin immunoprecipitation (ChIP). Overexpression of MsDREB6.2 increased the expression of a key cytokinin (CK) catabolism gene, MdCKX4a, which led to a significant reduction in endogenous CK levels, and caused a decrease in shoot:root ratio in transgenic apple plants. Overexpression of MsDREB6.2 resulted in a decrease in stomatal aperture and density and an increase in root hydraulic conductance (L ), and thereby enhanced drought tolerance in transgenic plants. Furthermore, manipulating the level of MsDREB6.2 expression altered the expression of two aquaporin (AQP) genes. The effect of the two AQP genes on L was further characterized using the AQP inhibitor HgCl . Based on these observations, we conclude that MsDREB6.2 enhances drought tolerance and that its function may be due, at least in part, to its influence on stomatal opening, root growth, and AQP expression. These results may have applications in apple rootstock breeding programs aimed at developing drought-resistant apple varieties.

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“…Micropropagated M. sieversii plants were pre-cultured in 1/2 Hoagland nutrient solution for 15 days and then transferred to full-strength Hoagland solution [52]. Plants with heights ranging from 25 to 30 cm were selected for treatments.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide analysis of the GH3 family in apple (Malus × domestica)

et al. 2013

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BackgroundAuxin plays important roles in hormone crosstalk and the plant’s stress response. The auxin-responsive Gretchen Hagen3 (GH3) gene family maintains hormonal homeostasis by conjugating excess indole-3-acetic acid (IAA), salicylic acid (SA), and jasmonic acids (JAs) to amino acids during hormone- and stress-related signaling pathways. With the sequencing of the apple (Malus × domestica) genome completed, it is possible to carry out genomic studies on GH3 genes to indentify candidates with roles in abiotic/biotic stress responses.ResultsMalus sieversii Roem., an apple rootstock with strong drought tolerance and the ancestral species of cultivated apple species, was used as the experimental material. Following genome-wide computational and experimental identification of MdGH3 genes, we showed that MdGH3s were differentially expressed in the leaves and roots of M. sieversii and that some of these genes were significantly induced after various phytohormone and abiotic stress treatments. Given the role of GH3 in the negative feedback regulation of free IAA concentration, we examined whether phytohormones and abiotic stresses could alter the endogenous auxin level. By analyzing the GUS activity of DR5::GUS-transformed Arabidopsis seedlings, we showed that ABA, SA, salt, and cold treatments suppressed the auxin response. These findings suggest that other phytohormones and abiotic stress factors might alter endogenous auxin levels.ConclusionPrevious studies showed that GH3 genes regulate hormonal homeostasis. Our study indicated that some GH3 genes were significantly induced in M. sieversii after various phytohormone and abiotic stress treatments, and that ABA, SA, salt, and cold treatments reduce the endogenous level of axuin. Taken together, this study provides evidence that GH3 genes play important roles in the crosstalk between auxin, other phytohormones, and the abiotic stress response by maintaining auxin homeostasis.

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Isolation and characterization of a dehydration responsive element binding factor MsDREBA5 in Malus sieversii Roem.

Cited by 15 publications

References 33 publications

The Banana Transcriptional Repressor MaDEAR1 Negatively Regulates Cell Wall-Modifying Genes Involved in Fruit Ripening

The Banana Transcriptional Repressor MaDEAR1 Negatively Regulates Cell Wall-Modifying Genes Involved in Fruit Ripening

Overexpression of MsDREB6.2 results in cytokinin‐deficient developmental phenotypes and enhances drought tolerance in transgenic apple plants

Genome-wide analysis of the GH3 family in apple (Malus × domestica)

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