Isolation and Characterization of a Peanut Maturity-Associated Protein

Bland, John M.; Lax, Alan R.

doi:10.1021/jf000307h

Cited by 19 publications

(9 citation statements)

References 24 publications

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“…The effect of roasting on mAb‐defined epitopes was compared by inhibition ELISA. The ELISA was also used to study whether Ara h 1 levels per gram of peanut increased with peanut maturation (instead of Ara h 1 concentration been kept the same as peanut grows and matures), as suggested by a previous report of a 62.5 kDa protein peak, most likely Ara h 1, that increased in size as the peanut matured [13].…”

Section: Introductionmentioning

confidence: 99%

Quantification of Ara h 1 in peanuts: why roasting makes a difference

Pomés

Butts

Chapman

2006

Clin Experimental Allergy

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Section: Introductionmentioning

confidence: 99%

Quantification of Ara h 1 in peanuts: why roasting makes a difference

Pomés

Butts

Chapman

2006

Clin Experimental Allergy

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“…Para determinação da atividade dos inibidores de tripsina e quimotripsina, foram triturados 10 g de sementes de cada genótipo de amendoim em multiprocessador, com posterior delipidação com 20 mL de acetona PA e 20 mL de hexano PA, obedecendo metodologia descrita por Bland & Lax (2000); em seguida, foram pesados 100 mg da amostra delipidada e acrescentado 1 mL de tampão Tris-HCl 50 mol L -1 , pH 8,5, incubado sob agitação durante a noite, a 4 ºC. O extrato foi …”

Section: Methodsunclassified

Prospecção de descritores para seleção de genótipos de amendoim tolerantes a pragas de grãos armazenados

Santos

Luz

Pinheiro

et al. 2014

Rev. bras. eng. agríc. ambient.

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5 R E S U M OAs inter-relações entre descritores bioquímicos e agronômicos foram investigadas em genótipos de amendoim visando identificar indicadores de seleção para tolerância a pragas de grãos armazenados, com base na capacidade inibitória para tripsina e quimotripsina. Dez genótipos foram cultivados em campo, em 2011/2012, e suas sementes utilizadas para os ensaios bioquímicos. Quatro descritores agronômicos foram utilizados para compor as análises de correlação. Sete genótipos revelaram alta capacidade inibitória para tripsina, com mais de 80% de inibição; contudo, para quimotripsina, observou-se baixa inibição em todos os genótipos, variando entre 4 e 12%. Nas análises de correlação não foram observadas inter-relações significativas entre os inibidores e as demais variáveis, com exceção de inibidor de tripsina x proteínas nas folhas, que revelou correlação negativa de alta magnitude indicando que alto teor de proteínas nas folhas pode reduzir o nível de inibidor de tripsina nas sementes. Este descritor poderia ser utilizado como indicador de seleção para tolerância a pragas de grãos armazenados, com o benefício de não destruir a semente. Prospecting of descriptors for selection of peanut genotypes tolerant to stored grain pests A B S T R A C TThe interrelations between biochemical and agronomic descriptors were investigated in peanut genotypes in order to identify selection indicators for tolerance to stored grain pests, based on the inhibitory capacity for trypsin and chymotrypsin. Ten genotypes were grown in field, in 2011/2012, and their seeds were further used for biochemical assays. Four agronomic traits were used in order to compose the correlation analysis. Seven genotypes showed high inhibitory capacity for trypsin with more than 80% inhibition; however, chymotrypsin inhibition was low in all genotypes, varying between 4 and 12%. No significant interrelationships between inhibitors and the other variables were seen in correlation analysis, except trypsin inhibitor x leaves protein, which revealed a negative correlation with high magnitude, indicating that a high protein content in the leaves can reduce the level of trypsin inhibitor in the seeds. This descriptor could be used as a selection indicator for tolerance to stored grain pests, adding the benefit of saving the seed.

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“…The sesame seeds used in this study were harvested in 2013 in Barbalha, CE, (07° 18'18 "S, 39° 18'07" W, 414 m), in a semiarid region of Brazilian northeastern. Seeds (3 g) of ten sesame genotypes (Table 1) were used for total protein extraction, starting with delipidation (Bland and Lax, 2000). Delipidated samples (100 mg) were homogeneized in 1 ml Tris-HCl 0.05 M buffer, pH 8.5 and centrifuged at 12.000 x g for 20 min at 4°C.…”

Section: Protein Extraction and Fractioningmentioning

confidence: 99%

Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hbner) (Lepidoptera: Pyralidae)

Gomes¹,

Santos²,

Albuquerque³

et al. 2015

Afr. J. Agric. Res.

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Biochemical and feeding assays were performed in order to assess the bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae. The biochemical assays were based on the ability of protease inhibitors (PI) present in sesame seeds to inhibit bovine pancreatic trypsin and digestive enzymes of insect larvae. The stability of the inhibitors was also assessed at different pH and temperature. The feeding bioassays were carried out in plates containing second instar larvae fed on diet with sesame protein extract. It was observed that all genotypes presented inhibition when tested with BPT, ranging from 51 to 90%, among them BRS Seda, CNPA G3 and CNPA G4 showed average of 69% of inhibitory activity for intestinal homogenate. The FII-fractions in these three genotypes showed thermal and pH stabilities at 40°C and 8.5, respectively. In feeding bioassay, P. interpunctella larvae were susceptible to SPCE of all three genotypes, revealing average mortality of 74% to SPCE and 45% to FII-fractions. Among all genotypes, BRS Seda was more effective to inhibition of digestive enzymes of insects and considered the more suitable genotype for further using in sesame breeding program to resistance to P. interpunctella.

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Isolation and Characterization of a Peanut Maturity-Associated Protein

Cited by 19 publications

References 24 publications

Quantification of Ara h 1 in peanuts: why roasting makes a difference

Quantification of Ara h 1 in peanuts: why roasting makes a difference

Prospecção de descritores para seleção de genótipos de amendoim tolerantes a pragas de grãos armazenados

Bioactivity of trypsin inhibitors from sesame seeds to control Plodia interpunctella larvae (Hbner) (Lepidoptera: Pyralidae)

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