Isolation and Characterization of A Novel Iron–Sulfur Oxidizing Bacterium Acidithiobacillus Ferrooxidans YQ-N3 and its Applicability in Coal Biodesulfurization

Abstract

Acidithiobacillus ferrooxidans is a chemotrophic, aerobic, acidophilic, and Gram-negative bacterium that plays a key role in iron and sulfur cycling and has a wide range of applications in the industrial field. A novel A. ferrooxidans strain, hereinafter referred to as strain “YQ-N3”, was isolated from sediments of a river polluted by acid mine drainage (AMD) of an abandoned mine in Shanxi, China. The whole genome sequencing results revealed that A. ferrooxidans YQ-N3 has a 3,217,720 bp genome, which is comprised of one circular chromosome and five circular plasmids (Plasmid A, Plasmid B, Plasmid C, Plasmid D, Plasmid E). Plasmid E, a new plasmid, had not been annotated in the reference database. A. ferrooxidans YQ-N3 had a close evolutionary relationship with A. ferrooxidans ATCC23270 and A. ferridurans JCM18981 and exhibited higher similarity in its genomic structure with A. ferrooxidans ATCC23270. Multiple genes related to environmental resistance and iron and sulfur metabolism were predicted from its genome. A. ferrooxidans YQ-N3 can remarkably increase the oxidation rate of Fe2+ and S0 and enhance the hydrophilicity of S0, which was supported by functional gene analysis and laboratory experiments. The biological desulfurization experiment demonstrated that A. ferrooxidans YQ-N3 can reduce the sulfur content in coal by removing pyrite sulfur and organic sulfur.

Desulfurization of coal using heterogeneous microbial species isolated from acid mine drainage, Talabira Coal Mines, Sambalpur, Odisha

“…Incubation of coal with microbes isolated from the Mines drainage water, Talabira mines significantly increased (t= 15.27, p≥0.05) the Total iron content in the medium compared to -1 control sterile water without any microbial consortia from 0.1mg l -1 on day 1 to 2.58 mg l on day 24. The findings of this study corroborate with the previous reports ( Ye et al, 2016;Li et al, 2023) where similar leaching of iron, following (Acharya et al, 2004He et al, 2013 incubation of coal sample with a pure culture of Acidithiobacillus ferrooxidans was observed. When investigated for the effect of different shaking speeds (experimental conditions 10% coal pulp density, particle size 180µm, and pH=1.5), the maximum desulfurization was achieved at 180 and 210 rpm with 20% sulphur removal in 5 days.…”