Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Sun, Sheng; Chen, Feng; Cao, Sheng; Li, Jiajia; Lei, Wen; Li, Guangwei; Song, Yue; Lü, Jian; Liu, Chuang; Qin, Jian-Ping; Li, Haiyan

doi:10.1186/s13567-014-0074-y

Cited by 39 publications

(31 citation statements)

References 41 publications

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“…aMPV subtype B have been reported in Iran [33,34]. In addition, aMPV subtype A and subtype C have been reported in China [35][36][37]. Again, geographic proximity and commercial exchanges of Pakistan with Iran and India rather than with China may explain the circulation of aMPV type B in Pakistan, although the PCR primers used here would not have allowed aMPV subtype C detection.…”

Section: Discussionmentioning

confidence: 91%

Molecular epidemiology of respiratory viruses in commercial chicken flocks in Pakistan from 2014 through to 2016

et al. 2019

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Background Viral diseases are a matter of great concern for poultry farmers in Pakistan. Multiple common viral respiratory diseases (CVRDs) cause huge economic losses in the poultry industry. The prevalence of CVRDs in many countries, including Pakistan, is not clearly understood. Results Incidences of 5 chicken respiratory viruses: avian influenza virus (AIV), Newcastle disease virus (NDV/AAVV-1), infectious bronchitis virus (IBV), avian metapneumovirus (aMPV) and infectious laryngotracheitis virus (ILTV) were assessed on commercial Pakistani farms with respiratory problems from 2014 through to 2016. While AIV and AAVV-1 were frequently detected (16 to 17% of farms), IBV and aMPV were rarely detected (in 3 to 5% of farms) and ILTV was not detected. We characterized H9 AIV of the G1 lineage, genotype VII AAVV-1, GI-13 IBV, and type B aMPV strains with very little genetic variability in the 2-year study period. Co-infections with AIV and AAVV-1 were common and wild type AAVV-1 was detected despite the use of vaccines. Control measures to limit the virus burden in chicken flocks are discussed. Conclusions Our data shows that AIV (H9), AAVV-1, IBV and aMPV are prevalent in commercial poultry in Pakistan. Further studies are necessary to assess circulating strains, economic losses caused by infections and coinfections of these pathogens, and the costs and benefits of countermeasures. Furthermore, veterinarians and farmers should be informed of the pathogens circulating in the field and hence advised on the use of vaccines.

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Section: Discussionmentioning

confidence: 91%

Molecular epidemiology of respiratory viruses in commercial chicken flocks in Pakistan from 2014 through to 2016

et al. 2019

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“…Subtype C aMPV was first reported in domestic turkeys in the United States (Senne, Edson, Pederson, & Panigrahy, ) and subsequently in wild birds such as American coots, American crows, Canada geese, cattle egrets and sparrows (Bennett et al., ; Turpin, Stallknecht, Slemons, Zsak, & Swayne, ). Subtype C aMPVs were also were detected in farmed ducks in France (Toquin et al., ) as well as in chickens (Wei et al., ) and ducks (Sun et al., ) in China, and pheasants in South Korea (Lee et al., ). It has been hypothesized that wild birds may act as a reservoir of subtype C aMPV and may provide a potential mechanism to spread aMPVs to poultry in other regions.…”

Section: Introductionmentioning

confidence: 99%

Avian metapneumovirus subtype C in Wild Waterfowl in Ontario, Canada

Jardine

Parmley

Buchanan

et al. 2018

Transbound Emerg Dis

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Avian metapneumovirus (aMPV) is an emerging poultry pathogen that has a significant economic impact on poultry production worldwide. The geographic range of the virus continues to expand, and wild birds have been implicated as reservoirs of aMPV that have the potential to spread the virus over long distances. Our objective was to determine the apparent prevalence of aMPV subtype C in wild waterfowl in Ontario, Canada. Wild waterfowl were captured in August and September, 2016 as part of routine migratory waterfowl population monitoring by the Ontario Ministry of Natural Resources and Forestry. Oropharyngeal and cloacal swabs were collected from each bird and placed together for aMPV testing using real-time RT-PCR. A total of 374 live wild birds from 23 lakes were sampled and tested for aMPV. Among all ducks tested, 84 (22%) were positive for aMPV. The proportion of samples that tested positive ranged from 0% in ring-necked ducks (Aythya collaris) and green-winged teal (Anas carolinensis) to 44% (8 of 18) in American black ducks (A. rubripes). Waterfowl positive for aMPV were found at 14 of 23 lakes in the study area and the percent positive at these 14 lakes ranged between 5% and 84%. Although subtype C aMPV has been detected in a variety of wild birds in North America, this is the first report of aMPV in wild ducks in Ontario, Canada. The high apparent prevalence, particularly in mallards and American black ducks (37 and 44%, respectively), suggests that these species may be important reservoirs of aMPV. Given the potential impact of aMPV on domestic poultry and the potential role of wild birds as reservoirs of the virus, further investigation of the geographic distribution, risk factors associated with aMPV carriage in wild waterfowl and potential role of other birds in the epidemiology of aMPV in Canada is warranted.

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“…There is a high similarity between subtype C aMPV and hMPV [5][6][7]. The infection of subtype C aMPV (aMPV/C) in Muscovy ducks has been reported in France and China [8,9] as well as in turkeys and wild birds in the USA [10], forming two distinct genetic lineages, one North American and the other Eurasian. In July 2010, an outbreak with severe respiratory symptoms occurred in meat chickens in southern China.…”

Section: Introductionmentioning

confidence: 99%

Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis

Quan

Wei

Hou

et al. 2020

Viruses

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Avian metapneumovirus subtype C (aMPV/C) causes an acute respiratory disease that has caused serious economic losses in the Chinese poultry industry. In the present study, we first explored the protein profile in aMPV/C-infected Vero cells using iTRAQ quantitative proteomics. A total of 921 of 7034 proteins were identified as significantly altered by aMPV/C infection. Three selected proteins were confirmed by Western blot analysis. Bioinformatics GO analysis revealed multiple signaling pathways involving cell cycle, endocytosis, and PI3K-Akt, mTOR, MAPK and p53 signaling pathways, which might participate in viral infection. In this analysis, we found that PLK2 expression was upregulated by aMPV/C infection and investigated whether it contributed to aMPV/C-mediated cellular dysfunction. Suppressing PLK2 attenuated aMPV/C-induced reactive oxygen species (ROS) production and p53-dependent apoptosis and reduced virus release. These results in a mammalian cell line suggest that high PLK2 expression correlates with aMPV/C-induced apoptosis and viral replication, providing new insight into the potential avian host cellular response to aMPV/C infection and antiviral targets.

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Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Cited by 39 publications

References 41 publications

Molecular epidemiology of respiratory viruses in commercial chicken flocks in Pakistan from 2014 through to 2016

Molecular epidemiology of respiratory viruses in commercial chicken flocks in Pakistan from 2014 through to 2016

Avian metapneumovirus subtype C in Wild Waterfowl in Ontario, Canada

Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis

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