Isolation and characterization of a high-efficiency marine diesel oil-degrading bacterium

Abstract

In the recent 50 years, marine oil spills had resulted in severe environmental pollution problems worldwide. In this study, 12 petroleum-degrading strains named MJ1 to MJ12, which can use diesel oil as the sole carbon source for growth, were isolated from the seawater in Jiaozhou Bay, China. Strain MJ4 has the highest diesel-degrading rate which is up to 26.54% in 5 days with the diesel oil concentration of 10 g/L. According to the BLAST research, 16SrRNA sequence of MJ4 showed 99% similarity to Bacillus megaterium strain. Single-factor experiments and response surface methodology were carried out to optimize the environmental factors and their reciprocal action for affecting the diesel oil degradation process of Bacillus sp. MJ4. Results of single-factor experiments revealed that the highest degradation rate was obtained with temperature of 28 °C, pH of 8.8, diesel oil concentration of 25 g/L, P/N ratio of 0.56, nitrogen and phosphorus dosage quantity of 0.35 g/L and 0.18 g/L, respectively. A nonlinear regression equation of diesel oil degradation rate and pH, temperature, P/N ratio was obtained. The model predicted the maximum degradation rate of 72.21% with temperature of 28 °C, pH of 8.88, P/N ratio of 0.31, respectively.

Bioremediation of Oil Spills: Current Status, Challenges, and Future Prospects

“…It has enabled scientists to study microbial diversity and perhaps the diversity of the community (Guerra et al, 2018). Most of the current studies regarding the search for oil-degrading bacteria have included 16s rRNA gene sequencing in the protocol which uses the differences in the sequences in the regions of the highly conserved 16s rRNA gene that encodes the smaller subunit of the bacterial ribosome to identify bacteria (Kim et al, 2018;Gao et al, 2021;Yu et al, 2022). Analyzing the sequences enables scientists to link the potential oil-degrading bacterium they have found to any well-studied bacteria and gain insights into its biology (David and Berry, 2017).…”

Reliability Analysis of Subsea Control System Using FMEA and FFTA

“…With the continuous depletion of oil and gas resources, the major oil companies are turning to the development of offshore oil and gas resources, and the demand for offshore oil exploit equipment is rapidly rising [1] [2]. As the mainstream mode of offshore oil and gas development, subsea oil and gas production systems are a series of equipment installed on the seabed to carry out oil and gas extraction operations, which have many advantages such as saving extraction costs, and improving oil and gas extraction efficiency [3]. As an important equipment for oil and gas development, the subsea control system is located in complex working environment, which not only be affected by multiple factors such as low temperature, high pressure and corrosion, but also be subject to risks such as falling objects from the sea and impacts from fishing nets, leading to failure modes such as leakage and corrosion [4] [5].…”

Natural and oil surface slicks as microbial habitats in marine systems: A mini review

“…An oil slick's lifetime strongly depends on its thickness, oil composition, wind speeds and related mixing conditions, but slick dimensions do not affect oil slick lifetime (Zeinstra-Helfrich et al, 2017). Thick oil slicks inhibit light penetration resulting in lower light intensities reaching the underlying water, affecting the growth of phototrophic organisms (Gao et al, 2021;Quigg et al, 2021). Oils slicks are hydrophobic and chemically complex mixtures containing several thousands of different compounds; hence, many compounds remain chemically uncharacterized through oil studies (Brussaard et al, 2016;Shi and Wu, 2021).…”