Isolation and characterization of a bacteriophage and its utilization against multi-drug resistant Pseudomonas aeruginosa-2995

Jamal, Muhsin; Andleeb, Saadia; Jalil, Fazal; Imran, Muhammad; Nawaz, Muhammad Asif; Hussain, Tahir; Ali, Muhammad Asad; Das, Chythanya Rajanna

doi:10.1016/j.lfs.2017.09.034

Cited by 37 publications

(32 citation statements)

References 32 publications

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“…3). Additionally, spectrophotometric monitoring of bacteria incubated with PA-PP protein showed that PAR50 growth was terminated, compared to control and PAR21 strain, which continued growing, analogous to a study showing that a hypothetical protein gp70 of Pseudomonas phage 14-1 has strong inhibitory effect on growth on solid medium 43 , furthermore, similar results were found by incubation of MDR P. aeruginosa MDR-PA1-5 strain with phage SL2 for 16 h 13 , and incubation of MDR P. aeruginosa 2995 strain with phage 44 .…”

Section: Discussionsupporting

confidence: 79%

Identification and characterization of phage protein and its activity against two strains of multidrug-resistant Pseudomonas aeruginosa

Al-Wrafy

Brzozowska

Górska

et al. 2019

Sci Rep

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Pseudomonas aeruginosa is an opportunistic pathogen with a capacity to develop antibiotic resistance, which underlies a larger proportion of hospital-acquired infections and higher morbidity and mortality, compared to other bacterial infections. Effective novel approaches for treatment of infections induced by this pathogen are therefore necessary. Phage therapy represents a promising alternative solution to eradicate antibiotic-resistant pathogens. Here, we investigated phage protein efficacy against multi-drug resistant (MDR) P. aeruginosa PAR21 and PAR50 strains isolated from diabetic foot ulcer patients. The results obtained using spot assay, zymography, spectrophotometry and scanning electron microscopy at low voltage (SEM-LV) indicate that the phage protein, PA-PP, exerts activity against P. aeruginosa PAR50 while having no impact on the PAR21 strain. Using LC-MS-MS/MS and comparative analysis of the peptide molecular mass with the protein sequence database, PA-PP was identified as a member of the serine protease family, a result corroborated by its ability to digest casein. We additionally showed a capacity of PA-PP to digest porin protein on the bacterial outer membrane (OM). Moreover, synergistic activity between PA-PP protein and piperacillin led to higher sensitivity of bacterial cells to this antibiotic. Our collective findings suggest that PA-PP targets porin protein on PAR50 OM, thereby increasing its sensitivity to specific antibiotics. The adverse effects observed on bacterial cells using SEM-LV suggest further roles of this protein that remain to be established.

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Section: Discussionsupporting

confidence: 79%

Identification and characterization of phage protein and its activity against two strains of multidrug-resistant Pseudomonas aeruginosa

Al-Wrafy

Brzozowska

Górska

et al. 2019

Sci Rep

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“…Therefore, phages that have high stability at various temperatures and pH values could be selected as some of the best candidates for application as sanitizers or alternative therapeutic agents (44). In this study, Bϕ-R656 and Bϕ-R1836 showed higher stability than other Pseudomonas phages (35, 36) at various temperatures (for 1 h) and pH values (for 30 days) (Fig. S2 and S3).…”

Section: Discussionmentioning

confidence: 56%

“…In the in vitro experiments, Bϕ-R656 and Bϕ-R1836 exhibited a relatively broader host spectrum than other phages targeting MDR P. aeruginosa strains (35–37), and these phages showed significant growth-inhibitory activity against host XDR-PA strains. Host range is an important parameter for initial screening of new effective phages against target bacteria, and this spectrum was confirmed using both spot tests and EOP assays (38, 39).…”

Section: Discussionmentioning

confidence: 95%

Two Novel Bacteriophages Improve Survival in Galleria mellonella Infection and Mouse Acute Pneumonia Models Infected with Extensively Drug-Resistant Pseudomonas aeruginosa

Jeon

Yong

2019

Appl Environ Microbiol

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Extensively drug-resistant Pseudomonas aeruginosa (XDR-PA) is a life-threatening pathogen that causes serious global problems. Here, we investigated two novel P. aeruginosa bacteriophages (phages), Bϕ-R656 and Bϕ-R1836, in vitro, in silico, and in vivo to evaluate the potential of phage therapy to control XDR-PA clinical strains. Bϕ-R656 and Bϕ-R1836 belong to the Siphoviridae family and exhibited broad host ranges which could lyse 18 (64%) and 14 (50%) of the 28 XDR-PA strains. In addition, the two phages showed strong bacteriolytic activity against XDR-PA host strains from pneumonia patients. The whole genomes of Bϕ-R656 and Bϕ-R1836 have linear double-stranded DNA of 60,919 and 37,714 bp, respectively. The complete sequence of Bϕ-R656 had very low similarity to the previously discovered P. aeruginosa phages in GenBank, but phage Bϕ-R1836 exhibited 98% and 91% nucleotide similarity to Pseudomonas phages YMC12/01/R24 and PA1/KOR/2010, respectively. In the two in vivo infection models, treatment with Bϕ-R656 and Bϕ-R1836 enhanced the survival of Galleria mellonella larvae (50% and 60%, respectively) at 72 h postinfection and pneumonia-model mice (66% and 83%, respectively) at 12 days postinfection compared with untreated controls. Treatment with Bϕ-R656 or Bϕ-R1836 also significantly decreased the bacterial load in the lungs of the mouse pneumonia model (>6 log10 CFU and >4 log10 CFU, respectively) on day 5. IMPORTANCE In this study, two novel P. aeruginosa phages, Bϕ-R656 and Bϕ-R1836, were evaluated in vitro, in silico, and in vivo for therapeutic efficacy and safety as an alternative antibacterial agent to control XDR-PA strains collected from pneumonia patients. Both phages exhibited potent bacteriolytic activity and greatly improved survival in G. mellonella larva infection and a mouse acute pneumonia model. Based on these results, we strongly predict that these two new phages could be used as fast-acting and safe alternative biological weapons against XDR-PA infections.

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“…A similar trend applied to moxifloxacin and gatifloxacin against MRSA, and gatifloxacin and norfloxacin against P. aeruginosa . This was compatible with the fact that certain antibiotics are more effective against biofilms of P. aeruginosa than other antibiotics [29,30,35,36,37,38]. For example, ciprofloxacin and ofloxacin were more effective against biofilms of P. aeruginosa than other antibiotics such as ceftazedim, gentamicin, imipenem, amikacin, azithromycin, and erythromycin [32].…”

Section: Discussionmentioning

confidence: 71%

In Vitro Comparison of Antibacterial and Antibiofilm Activities of Selected Fluoroquinolones against Pseudomonas aeruginosa and Methicillin-Resistant Staphylococcus aureus

et al. 2019

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An in vitro overview of the inhibitory effects of selected fluoroquinolones against planktonic and biofilm cells of the methicillin-resistant Staphylococcus aureus (MRSA) strain American type culture collection (ATCC) 43300 and the Pseudomonas aeruginosa strain ATCC 27853 was carried out. Biofilm cells of both strains were less susceptible to the selected antibiotics than their planktonic counterparts. In addition, certain antibiotics were more effective against biofilm cells, while others performed better on the planktonic cells. Against P. aeruginosa, ciprofloxacin was the most potent on both planktonic and biofilm cells, whereas ofloxacin was the least potent on both biofilm and planktonic cells. Moxifloxacin and gatifloxacin were the most potent against both planktonic and biofilm MRSA bacteria, however, not in the same order of activity. Norfloxacin was the least active when tested against both planktonic and biofilm cells. The results of this work are expected to provide insight into the efficacy of various fluoroquinolones against MRSA and Pseudomonas aeruginosa biofilms. This study could form the basis for future clinical studies that could recommend special guidelines for the management of infections that are likely to involve bacteria in their biofilm state.

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Isolation and characterization of a bacteriophage and its utilization against multi-drug resistant Pseudomonas aeruginosa-2995

Cited by 37 publications

References 32 publications

Identification and characterization of phage protein and its activity against two strains of multidrug-resistant Pseudomonas aeruginosa

Identification and characterization of phage protein and its activity against two strains of multidrug-resistant Pseudomonas aeruginosa

Two Novel Bacteriophages Improve Survival in Galleria mellonella Infection and Mouse Acute Pneumonia Models Infected with Extensively Drug-Resistant Pseudomonas aeruginosa

In Vitro Comparison of Antibacterial and Antibiofilm Activities of Selected Fluoroquinolones against Pseudomonas aeruginosa and Methicillin-Resistant Staphylococcus aureus

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