Isolation and Characterization of a Putative Intestinal Stem Cell Fraction From Mouse Jejunum

Dekaney, Christopher M.; Rodriguez, Juana María; Graul, Małgorzata; Henning, Susan J.

doi:10.1053/j.gastro.2005.08.011

Cited by 92 publications

(110 citation statements)

References 43 publications

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“…Toward this goal, steps have been taken to isolate and characterize ISCs. Sorting of " side population " cells yielded a side population fraction that is far from pure, 19,20 but it appears that the number of CD45-negative side population cells is a reasonable surrogate for stem cell number. 21 Development of better isolation methods and functional assays of ISCs remains a pivotal challenge.…”

Section: Commentaries Nature Publishing Groupmentioning

confidence: 98%

Recent insights into the integration of the intestinal epithelium within the mucosal environment in health and disease

Blumberg

Nusrat

et al. 2008

Mucosal Immunology

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Section: Commentaries Nature Publishing Groupmentioning

confidence: 98%

Recent insights into the integration of the intestinal epithelium within the mucosal environment in health and disease

Blumberg

Nusrat

et al. 2008

Mucosal Immunology

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“…Intestinal cells were obtained by using established methods (14,59). In brief, the small intestine was placed in Hanks balanced salt solution containing 5% (vol/vol) fetal bovine serum (CSL, Ltd., Melbourne, Australia) and 1 mM dithiothreitol (Sigma).…”

Section: Detection Of Rotavirus In Organs and Samples From Rotavirus-mentioning

confidence: 99%

Rotavirus Infection Accelerates Type 1 Diabetes in Mice with Established Insulitis

Graham

Sanders

Tan

et al. 2008

J Virol

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Infection modulates type 1 diabetes, a common autoimmune disease characterized by the destruction of insulin-producing islet ␤ cells in the pancreas. Childhood rotavirus infections have been associated with exacerbations in islet autoimmunity. Nonobese diabetic (NOD) mice develop lymphocytic islet infiltration (insulitis) and then clinical diabetes, whereas NOD8.3 TCR mice, transgenic for a T-cell receptor (TCR) specific for an important islet autoantigen, show more rapid diabetes onset. Oral infection of infant NOD mice with the monkey rotavirus strain RRV delays diabetes development. Here, the effect of RRV infection on diabetes development once insulitis is established was determined. NOD and NOD8.3 TCR mice were inoculated with RRV aged >12 and 5 weeks, respectively. Diabetes onset was significantly accelerated in both models (P < 0.024), although RRV infection was asymptomatic and confined to the intestine. The degree of diabetes acceleration was related to the serum antibody titer to RRV. RRV-infected NOD mice showed a possible trend toward increased insulitis development. Infected males showed increased CD8 ؉ T-cell proportions in islets. Levels of ␤-cell major histocompatibility complex class I expression and islet tumor necrosis factor alpha mRNA were elevated in at least one model. NOD mouse exposure to mouse rotavirus in a natural experiment also accelerated diabetes. Thus, rotavirus infection after ␤-cell autoimmunity is established affects insulitis and exacerbates diabetes. A possible mechanism involves increased exposure of ␤ cells to immune recognition and activation of autoreactive T cells by proinflammatory cytokines. The timing of infection relative to mouse age and degree of insulitis determines whether diabetes onset is delayed, unaltered, or accelerated.

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“…While considerable efforts have been made to define the genetic signature of small intestine epithelial cells (2,9,10,15,17,18,25,26,30,37,(41)(42)(43)(44), fewer studies have focused on identifying genetic biomarkers of colonic epithelial stem cells (CESCs) (11,13,36,39). The concept of two populations of CESCs has been postulated: one is a slowly dividing labelretaining population, and the other is an actively dividing high-turnover population (19).…”

mentioning

confidence: 99%

Distinct levels of Sox9 expression mark colon epithelial stem cells that form colonoids in culture

Ramalingam

Daughtridge

Johnston

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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Isolation and Characterization of a Putative Intestinal Stem Cell Fraction From Mouse Jejunum

Cited by 92 publications

References 43 publications

Recent insights into the integration of the intestinal epithelium within the mucosal environment in health and disease

Recent insights into the integration of the intestinal epithelium within the mucosal environment in health and disease

Rotavirus Infection Accelerates Type 1 Diabetes in Mice with Established Insulitis

Distinct levels of Sox9 expression mark colon epithelial stem cells that form colonoids in culture

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