Isolation and characterization of a carbofuran-degrading strainNovosphingobiumsp. FND-3

Abstract

A gram-negative Novosphingobium sp. strain FND-3 capable of degrading carbofuran was isolated and characterized. The carbofuran-degrading ability of strain FND-3 was investigated under various culture conditions. Strain FND-3 showed a high average carbofuran-degrading rate of 28.6 mg L(-1) h(-1) in mineral salts medium with 100 mg L(-1) carbofuran. GC/MS analysis pointed out the presence of several unknown metabolites. One hydrolyzate was identified as 2-hydroxy-3-(3-methypropan-2-ol) phenol via hydrolysis of carbofuran phenol. The appearance of another metabolite with M(+) of 180 m/z indicated that the hydroxylation of carbofuran occurred at the aromatic ring. One novel degrading product with M(+) of 239 m/z was identified as 2-hydroxy-3-(3-methylpropan-2-ol) benzene-N-methylcarbamate via hydrolyzing at the ether bond of furanyl ring of carbofuran. Strain FND-3 was also able to degrade other N-methylcarbamate pesticides.

Phenanthrene biodegradation by sphingomonads and its application in the contaminated soils and sediments: A review

“…Sphingomonads are able to degrade other recalcitrant compounds (Basta et al, 2005;Peng et al, 2008), including azo dyes (Stolz, 1999), chlorinated phenols (Cassidy et al, 1999;Crawford and Ederer, 1999;Fujii et al, 2001;Wittmann et al, 1998), dibenzo-and carbofurans (Feng et al, 1997;Keim et al, 1999;Wittich et al, 1992Wittich et al, , 1999, insecticides (Nagata et al, 2007(Nagata et al, , 1999, and herbicides (Adkins, 1999;Bending et al, 2003;Feng et al, 1997;Kim et al, 2005;Kohler, 1999;Sørensen et al, 2001;Weissenfels et al, 1991;Yan et al, 2007). Other compounds broken down and assimilated by sphingomonads are polysaccharides in addition to mono-and disaccharides, for example sugars like arabinose, fucose, galactose, lactose, mannose, melobiose, sucrose, trehalose, and xylose (Balkwill et al, 1997a,b;Denner et al, 2001;Fujii et al, 2001).…”

Interaction Between Pesticide and Soil Microorganisms and Their Degradation: A Molecular Approach

“…Comparing with standard microbiological methods, the molecular techniques provide us with a more comprehensive interpretation of the in situ microbial community and its response to both engineered bioremediation and natural attenuation processes (Brockman 1995 ). Different microbial enzymes with the capacity to hydrolyze pesticides have been identifi ed (Yan et al 2007 ) such as organophosphorus hydrolase (OPH, encoded by the opd gene). This gene has been found in bacterial strains that can use organophosphate pesticides as carbon source.…”

Characterization of polyhydroxyalkanoates (PHAs) biosynthesis by isolatedNovosphingobiumsp. THA_AIK7 using crude glycerol

“…THA_AIK7. Most of the bacteria of the genus Novosphingobium have been of special interest because of the ability to degrade toxic compounds and other chemicals such as polycyclic aromatic hydrocarbons (PAHs), polychlorophenol, carbofuran, and carbazole [15,35,42,44]. Till now, only 1 species of Novosphingobium has been reported as a PHAs producer.…”