Isolation and characterization of a micromycete, a producer of neutral catechol oxidases, from tropical soils with elevated dioxine content

Vasil'chenko, L. G.; Королева, О. В.; Степанова, Елена В.; Ландесман, Е. О.; Rabinovich, M. A.

doi:10.1007/bf02738042

Cited by 5 publications

(3 citation statements)

References 57 publications

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“…These laccase producing fungi include Trametes (Coriolus) versicolor, T.villosa, T. hirsute, T. gallica, T. ochracea, Cerrena maxima, Phlebia radiata, Lentinus tigrinus, Coriolopsis polyzona and Pleurotus eryngii. Laccases are also reported in saprophytic ascomycetes of com-posts (Myceliophthora thermophila, Curvularia, Penicillium, Aspergillus and Chaetomium thermophile) and in the soil hyphomycete Mycelia sterlia INBI 2-26 [6,7].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Laccase Production from <i>Penicillium martensii </i>NRC 345

Elshafei¹,

Hassan²,

Haroun³

et al. 2012

ALS

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Laccase production by Penicillium martensii NRC 345 was optimized. Eight media with different components were screened. The enzyme formed by P. martensii NRC 345 was detected mainly exocellularly under shake culture condition. Laccase formation reaches its maximum value with specific activities of about 7.18 U/mg protein at the twenty-sixth day of incubation at pH 5.5 and 30°C. Galactose (5 g/l) and sodium nitrate (0.2 g/l) were found to be the best carbon and nitrogen sources for laccase formation, respectively. Replacement of galactose instead of glucose at the same concentration increased laccase production by about more than ten-fold. Among the various wastes used wheat bran induces the highest laccase production with specific activity of 39.52 U/mg protein. Tween-80 and CuSO 4 .5H 2 O, were also tested for laccase induction.

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Section: Introductionmentioning

confidence: 99%

Optimization of Laccase Production from <i>Penicillium martensii </i>NRC 345

Elshafei¹,

Hassan²,

Haroun³

et al. 2012

ALS

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“…The major constituent of lignocellulose, cellulose, is a homo-polymer of cellobiose (1,4-β-dimer of D-glucose) moieties, whereas the second larger constituent, lignin is composed of condensed phenolic structures like coniferyl, sinapyl, and p-coumaryl alcohols. Fungi decompose lignin to various lowmolecular-mass quinones, semiquinones and phenoxy radicals [20], while cellulose is simultaneously decomposed by fungal cellulases to cellobiose and glucose as the major products [21]. Cellobiose dehydrogenase links both of these processes and by this means prevents the accumulation of toxic and highly reactive quinones and aromatic radicals, reducing them back to the phenolic structures at the expense of coupled oxidation of cellobiose molecules.…”

Section: Cellobiose Dehydrogenasea Versatile Fungal Enzyme Able To Rementioning

confidence: 99%

Phenolic antioxidants and their role in quenching of reactive molecular species in the human skin injury

et al. 2015

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A large percentage of the population suffers from impaired wound healing related problems that are partly attributed to the continuous production and exposure of wounds to reactive species. The skin is prone to many adverse effects when e.g. damaged, exposed to ionizing UV radiation and/or environmental reactive pollutants. The reactive molecular species attacking the skin in excessive quantities quickly overwhelm tissue antioxidants and other oxidant-degrading pathways. The uncontrolled release of these reactive species is involved in the oxidation of biomolecules including lipids which in turn results in a number of human skin disorders. Phenolic antioxidants are emerging as contenders removing reactive species produced in these chronic wounds, thus accelerating the healing process. Building on this knowledge, a novel physiologically active wound dressing containing both phenolic antioxidants and an enzyme (cellobiose dehydrogenase) for the continuous recycling of oxidized phenolic antioxidants has been developed recently. This novel approach should protect the wound from damage and will help to create ideal conditions for the successful healing of chronic wounds.

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“…The laccase-negative fungal strain INBI 2-26(−) was isolated from a laccase-producing association of nonsporulating fungi, inhabiting dioxin-contaminated Vietnamese soil [25], by repetitive protoplast inoculation onto a solid selective guaiacol-containing medium [11]. The strain was identified as Chaetomium species by sequencing its ITS1 and ITS2 fragments of rRNA gene (EMBL accession No AJ620951) [7].…”

Section: Organism Culture Conditions Enzymesmentioning

confidence: 99%

Cellobiose dehydrogenase of Chaetomium sp. INBI 2‐26(–): Structural basis of enhanced activity toward glucose at neutral pH

Vasil'chenko

Karapetyan

Yershevich

et al. 2011

Biotechnology Journal

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Cellobiose dehydrogenase (CDH) is an extracellular fungal flavocytochrome specifically oxidizing cellooligosaccharides and lactose to corresponding (-lactones by a variety of electron acceptors. In contrast to basidiomycetous CDHs, CDHs of ascomycetes also display certain activity toward glucose. The objective of this study was to establish the structural reasons of such an activity of CDH from mesophilic ascomycete Chaetomium sp. INBI 2-26 (ChCDH). The complete amino acid sequence of ChCDH displayed high levels of similarity with the amino acid sequences of CDHs from the thermophilic fungi Thielavia heterotallica and Myriococcum thermophilum. Peptide mass fingerprinting of purified ChCDH provided evidence for the oxidation of methionine residues in the FAD-domain. Comparative homology modeling of the structure of the ChCDH FAD-domain in complex with the transition state analog based on the structure of the same complex of basidiomycetous CDH (1NAA) as template indicated possible structural reasons for the enhanced activity of ascomycetous CDHs toward glucose at neutral pH, which is a prerequisite for application of CDH in a variety of biocompatible biosensors and biofuel cells.

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Isolation and characterization of a micromycete, a producer of neutral catechol oxidases, from tropical soils with elevated dioxine content

Cited by 5 publications

References 57 publications

Optimization of Laccase Production from <i>Penicillium martensii </i>NRC 345

Optimization of Laccase Production from <i>Penicillium martensii </i>NRC 345

Phenolic antioxidants and their role in quenching of reactive molecular species in the human skin injury

Cellobiose dehydrogenase of Chaetomium sp. INBI 2‐26(–): Structural basis of enhanced activity toward glucose at neutral pH

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