Isolation and Characterization of a Virus Associated with Arthritis of Chickens

Some observations on the isolation and cultivation of avian reoviruses

“…CAM inoculation was also favoured by Spradbrow and Baines (1974) who made comparisons with CEF and CK cell cultures. YS inoculation was found to be the most sensitive when compared with AC and CEK by Glass et al (1973) and with AC inoculation by Carboni et al (1975). CK, CEF and duck embryo fibroblast cultures were used by Mustaffa-Babjee et al (1973) (1976) used both CK and CELi cells and obtained quicker and more pronounced CPE with the latter.…”

“…Recently, reoviruses have been incriminated in a stunting and leg weakness syndrome (Vertommen et al, 1980). Isolation of avian reoviruses from field material has been made by inoculating suspected material into embryonated chicken eggs via the yolk sac (YS), allantoic cavity (AC) or chorioallantoic membrane (CAM) routes (Deshmukh and Pomeroy, 1969;Glass et al, 1973;Mustaffa-Babjee et al, 1973;Spradbrow and Baines, 1974;and Carboni et al, 1975), or by inoculation of chick embryo fibroblast (CEF), lung (CELu), liver (CELi) or kidney (CEK) cell cultures, or chicken kidney (CK) cell cultures (MustaffaBabjeeerai, 1973;Spradbrow and Baines, 1974;Jones et al, 1975;McFerranefa£, 1976).…”

Development of a reverse transcription loop-mediated isothermal amplification assay for visual detection of avian reovirus

“…The 10 gene segments are named as large (L1, L2, L3), medium (M1, M2, M3), and small (S1, S2, S3, S4) based on their size (Benavente & Martinez-Costas, 2007). ARV infects chicken, turkeys, ducks, and other avian species, and causes several disease syndromes including viral arthritis and tendosynovitis, stunting syndrome, respiratory disease, malabsorption syndrome, and diseases of the central nervous system (Petek et al, 1967;Gershowitz & Wooley, 1973;Glass et al, 1973;McFerran et al, 1976;Page et al, 1982;Hieronymus et al, 1983;Robertson & Wilcox, 1986;Van de Zande & Kuhn, 2007). Moreover, co-infections of ARV with other immune suppressive pathogens including avian reticuloendotheliosis virus, chicken anaemia virus, and avian leucosis virus lead to diminished weight gains, poor feed conversion and reduced marketability of affected birds, which results in considerable economic losses.…”

Anti-neoplastic effect of avian reovirus σ C protein on Rous sarcoma virus-induced tumors in chicken

“…Avian reoviruses (ARVs) differ from their mammalian counterparts in their lack of hemagglutination activity (Glass et al, 1973), their ability to induce cell fusion (Bodelon et al, 2001), and association with naturally occurring pathological conditions in chicken and turkeys (Robertson and Wilcox, 1986). ARVs cause disease conditions like reovirus-induced arthritis, chronic respiratory disease, and malabsorption syndrome in poultry (Fahey and Crawley, 1954;Hieronymus et al, 1983;Kibenge and Wilcox, 1983).…”