Isolation and Characterization of a Sagiyama Virus from Domestic Pigs

Chang, Chia‐Yi; Huang, Chin‐Cheng; Huang, Tien-Shine; Deng, Ming-Chung; Jong, Ming-Hwa; Wang, Fun-In

doi:10.1177/104063870601800203

Cited by 15 publications

(6 citation statements)

References 19 publications

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“…SAGV and GETV are two members of the Alphavirus genus of the family Togaviridae. GETV is widely distributed in southeast Asia and northern Australia along the Pacific Ocean [20][21][22][23][24]. GETV has been isolated from various mosquito species of the genera Culex, Aedes, and Armigeres [18].…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD)

Hu¹,

Zheng²,

Zhang

et al. 2012

BMC Microbiol

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BackgroundThe identification of new virus strains is important for the study of infectious disease, but current (or existing) molecular biology methods are limited since the target sequence must be known to design genome-specific PCR primers. Thus, we developed a new method for the discovery of unknown viruses based on the cDNA - random amplified polymorphic DNA (cDNA-RAPD) technique. Getah virus, belonging to the family Togaviridae in the genus Alphavirus, is a mosquito-borne enveloped RNA virus that was identified using the Virus-Discovery-cDNA RAPD (VIDISCR) method.ResultsA novel Getah virus was identified by VIDISCR from suckling mice exposed to mosquitoes (Aedes albopictus) collected in Yunnan Province, China. The non-structural protein gene, nsP3, the structural protein gene, the capsid protein gene, and the 3'-untranslated region (UTR) of the novel Getah virus isolate were cloned and sequenced. Nucleotide sequence identities of each gene were determined to be 97.1–99.3%, 94.9–99.4%, and 93.6–99.9%, respectively, when compared with the genomes of 10 other representative strains of Getah virus.ConclusionsThe VIDISCR method was able to identify known virus isolates and a novel isolate of Getah virus from infected mice. Phylogenetic analysis indicated that the YN08 isolate was more closely related to the Hebei HB0234 strain than the YN0540 strain, and more genetically distinct from the MM2021 Malaysia primitive strain.

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Section: Discussionmentioning

confidence: 99%

Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD)

Hu¹,

Zheng²,

Zhang

et al. 2012

BMC Microbiol

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“…A VEEV replicon vector has been used to express HA from the human Hong Kong H5N1 influenza A isolate (A/HK/ 156/97) and shown to protect chickens against a challenge with the homologous H5N1 virus (Schultz-Cherry et al, 2000). So far, Sagiyama virus is the only alphavirus found in swine and is geographically restricted to Asia (Chang et al, 2006). Importantly, pigs can be infected by VEEV and display a transient viremia (Dickerman et al, 1973), suggesting that the VEEV replicon vector can be used to develop vaccine candidates for the swine industry.…”

Section: Alphavirus-based Siv Vaccinesmentioning

confidence: 99%

Swine influenza vaccines: current status and future perspectives

Richt

2010

Anim. Health. Res. Rev.

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Swine influenza is an important contagious disease in pigs caused by influenza A viruses. Although only three subtypes of influenza A viruses, H1N1, H1N2 and H3N2, predominantly infect pigs worldwide, it is still a big challenge for vaccine manufacturers to produce efficacious vaccines for the prevention and control of swine influenza. Swine influenza viruses not only cause significant economic losses for the swine industry, but are also important zoonotic pathogens. Vaccination is still one of the most important and effective strategies to prevent and control influenza for both the animal and human population. In this review, we will discuss the current status of swine influenza worldwide as well as current and future options to control this economically important swine disease.

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“…GETV was first isolated from a Culex mosquito in Malaysia in 1955, and later found in a variety of mosquito species and animals [3–7]. The GETV lifecycle is similar to that of the Japanese encephalitis virus; it is transmitted via Culex mosquitoes and amplified in domestic pigs [8]. It can cause death in young piglets, miscarriage in pregnant sows, and mild illness in horses [9–11].…”

Section: Introductionmentioning

confidence: 99%

First isolation and characterization of Getah virus from cattle in northeastern China

Líu

Zhang

et al. 2019

BMC Vet Res

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Background Getah virus (GETV) is a neglected mosquito-borne Alphavirus that causes pyrexia, body rash, and leg oedema in horses and foetal death and reproductive disorders in pigs. Infected animals may play a critical role in the amplification and circulation of the virus. The present study aimed to investigate GETV infection in clinically infected cattle and vector mosquito species in northeastern China. Results Serum samples were collected from beef cattle that presented sudden onset of fever in forest grazing areas, and metagenomic sequencing was conducted, revealing 29 contigs from ten serum samples matching the GETV genome. Quantitative RT-PCR (RT-qPCR) was performed with GETV RNA from 48 beef cattle serum samples, showing that the overall prevalence of GETV in the beef cattle samples was 6.25% (3/48). Serological investigation indicated that GETV neutralizing antibodies were detected in 83.3% (40/48, 95% CI 67–100) of samples from the study region. The GETV JL1808 strain was isolated from clinically infected cattle showing fever. Sequence comparisons showed high identity with the HuN1 strain, a highly pathogenic swine epidemic isolate obtained in Hunan province in 2017, at the nucleotide level (99.5%) and at the deduced amino acid level (99.7–99.9%). The phylogenetic analysis of JL1808 clustered in Group III, and also revealed a close genetic relationship with the HuN1 strain. Additionally, about 12,000 mosquitoes were trapped in this region. The presence of GETV infection was detected in mosquitoes, suggesting that the minimum infection rate (MIR) was 1.50‰, with MIRs of 1.67‰ in Culex pseudovishnui , 1.60‰ in Culex tritaeniorhynchus , and 1.21‰ in Anopheles sinensis . Conclusions To the best of our knowledge, this is the first report of GETV infection in cattle. These results demonstrated that a highly pathogenic, mosquito-borne swine GETV can infect and circulate in cattle, implying that it is necessary to conduct surveillance of GETV infection in animals in northeastern China. Electronic supplementary material The online version of this article (10.1186/s12917-019-2061-z) contains supplementary material, which is available to authorized users.

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Isolation and Characterization of a Sagiyama Virus from Domestic Pigs

Cited by 15 publications

References 19 publications

Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD)

Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD)

Swine influenza vaccines: current status and future perspectives

First isolation and characterization of Getah virus from cattle in northeastern China

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