Isolation and Characterization of a Dominant Dwarf Gene, D-h, in Rice

Piao, Rihua; Chu, Sang-Ho; Jiang, Wenzhu; Yu, Yoye; Jin, Yongmei; Woo, Mi-Ok; Lee, Joohyun; Kim, Sunghan; Koh, Hee‐Jong

doi:10.1371/journal.pone.0086210

Cited by 23 publications

(15 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plant height is determined by complex genetic networks, and a variety of plant height-related genes have been identified and cloned in recent years (Yamaguchi 2008;Piao et al 2014). Most of these genes are involved in phytohormone synthesis and/or signal transduction; gibberellins (GAs) and brassinosteroids (BRs) are two major hormones involved in plant height regulation (Wang and Li 2008).…”

Section: Introductionmentioning

confidence: 99%

Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

Cheng

Gao

Feng

et al. 2016

Journal of Integrative Agriculture

View full text Add to dashboard Cite

Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M 2 population. Two of these mutants, Gmdwf1 (Glycine max dwarf 1) and Gmdwf2 (Glycine max dwarf 2), were investigated in this study. Genetic analysis showed that both mutants were inherited in a recessive manner and their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping. Further fine mapping study proved that the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously. The dwarfism of Gmdwf1 could not be rescued by gibberellin (GA) and brassinolide (BR) treatments, which indicated that the biosynthesis of these hormones was not deficient in Gmdwf1.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

Cheng

Gao

Feng

et al. 2016

Journal of Integrative Agriculture

View full text Add to dashboard Cite

show abstract

“…In rice, there have been several attempts to identify new genes for semi-dwarfism/dwarfism as well as different alleles of SD1 through both induced and spontaneous mutations. Dominant or semi-dominant rice mutants obtained through chemical mutagenesis, such as KL908 carrying the D53 gene [ 23 ], DMF-1 with the Ssi1 gene [ 24 ], Y98149 with Sdd(t) [ 25 ], Twisted dwarf 1-1 ( Tid1-1 ) encoding the α-tubulin protein [ 26 ], LB4D carrying the LB4D gene [ 27 ], Slr1-d with a gain of function in the SLR1 protein [ 3 , 28 ], HD1 carrying the d-h gene [ 29 ], Bdt1 obtained by DNA transposon [ 30 ], SV14 with the Shortened Basal Internodes ( SBI ) gene on chromosome 5 [ 13 ], were reported in japonica genotypes. The semi-dominant quantitative trait loci (QTL), qDH1 , was identified on chromosome 1 in upland rice landrace, Kaowenghan [ 31 ], while, in indica , a recessive gene, sd-c , located at the centromeric region of chromosome 12 has been reported in indica cv.…”

Section: Discussionmentioning

confidence: 99%

Discovery of a Novel Induced Polymorphism in SD1 Gene Governing Semi-Dwarfism in Rice and Development of a Functional Marker for Marker-Assisted Selection

Bhuvaneswari

Krishnan

Ellur

et al. 2020

Plants

View full text Add to dashboard Cite

The semi-dwarfing allele, sd1-d, has been widely utilized in developing high-yielding rice cultivars across the world. Originally identified from the rice cultivar Dee-Geo-Woo-Gen (DGWG), sd1-d, derived from a spontaneous mutation, has a 383-bp deletion in the SD1 gene. To date, as many as seven alleles of the SD1 gene have been identified and used in rice improvement, either with a functional single-nucleotide polymorphism (SNP), with insertion–deletions (InDels), or both. Here, we report discovery of a novel SNP in the SD1 gene from the rice genotype, Pusa 1652. Genetic analysis revealed that the inheritance of the semi-dwarfism in Pusa 1652 is monogenic and recessive, but it did not carry the sd1-d allele. However, response to exogenous gibberellic acid (GA3) application and the subsequent bulked segregant and linkage analyses confirmed that the SD1 gene is involved in the plant height reduction in Pusa 1652. Sequencing of the SD1 gene from Pusa 1652 revealed a novel transition in exon 3 (T/A) causing a nonsense mutation at the 300th codon. The stop codon leads to premature termination, resulting in a truncated protein of OsGA20ox2 obstructing the GA3 biosynthesis pathway. This novel recessive allele, named sd1-bm, is derived from Bindli Mutant 34 (BM34), a γ-ray induced mutant of a short-grain aromatic landrace, Bindli. BM34 is the parent of an aromatic semi-dwarf cultivar, Pusa 1176, from which Pusa 1652 is derived. The semi-dwarfing allele, sd1-bm, was further validated by developing a derived cleaved amplified polymorphic sequence (dCAPS) marker, AKS-sd1. This allele provides an alternative to the most widely used sd1-d in rice improvement programs and the functional dCAPS marker will facilitate marker-assisted introgression of the semi-dwarf trait into tall genotypes.

show abstract

“…The dominant wheat gene Rht10 has been successfully used in wheat breeding (Kurkiev, ). Very few dominant dwarfing genes have been reported in rice (Piao et al., ; Qin et al., ; Sunohara et al., ; Wei, Xu, Li, Qian, & Zhu, ; Yang, Zheng, et al., ). D53 was the lone rice‐dominant dwarf mutant for a long time (Wei et al., ).…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis and fine mapping of a dominant dwarfness gene from wild rice (Oryza barthii)

Zhao

Zhang

et al. 2018

Plant Breeding

View full text Add to dashboard Cite

Plant architecture has been proposed as a means to enhance the potential yield of rice, especially by reducing height to provide lodging resistance. In this study, we developed a near‐isogenic line (NIL) using cultivar ‘Dianjingyou 1’ (DJY1) as a recipient parent and wild rice (Oryza barthii) as the donor parent. The NIL had semi‐dwarf stature and more tillers than DJY1. Cytological examination showed decreased numbers of cells in the stems of the NIL compared to DJY1. Genetic analysis indicated that this phenotype was controlled by a newly identified dominant dwarf gene, tentatively named as Dd7. A large population derived from the hybrid NIL‐Dd7/DJY1 was developed to fine‐map Dd7. Based on the physical location of molecular markers, the Dd7 locus was finally delimited to an 82‐kb region in chromosome 7. Gene prediction identified 14 open reading frames (ORFs) within this region. NIL‐Dd7 seems to confer no undesirable pleiotropic effects and therefore has potential value for rice breeding.

show abstract

Isolation and Characterization of a Dominant Dwarf Gene, D-h, in Rice

Cited by 23 publications

References 34 publications

Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean

Discovery of a Novel Induced Polymorphism in SD1 Gene Governing Semi-Dwarfism in Rice and Development of a Functional Marker for Marker-Assisted Selection

Genetic analysis and fine mapping of a dominant dwarfness gene from wild rice (Oryza barthii)

Contact Info

Product

Resources

About