Isolation and characterization of a virulent Vibrio sp. bacterium from clams (Meretrix meretrix) with mass mortality

Yue, Xin; Liu, Baozhong; Sun, Li

doi:10.1016/j.jip.2010.10.006

Cited by 20 publications

(4 citation statements)

References 44 publications

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“…Bacterial proliferation in the hepatopancreas has been reported for pathogenic vibrios in spiny lobster phyllosomas (6,12,18,52), penaeid shrimp (1,23,35), and molluscs (54). In the present study, proliferation of the pathogen occurred in the distal ends of the hepatopancreas 12 h after exposure.…”

Section: Discussionsupporting

confidence: 60%

Pathogenicity and Infection Cycle of Vibrio owensii in Larviculture of the Ornate Spiny Lobster (Panulirus ornatus)

Goulden

Hall

Bourne

et al. 2012

Appl Environ Microbiol

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bThe type strain of Vibrio owensii (DY05) was isolated during an epizootic of aquaculture-reared larvae (phyllosomas) of the ornate spiny lobster (Panulirus ornatus). V. owensii DY05 was formally demonstrated to be the etiological agent of a disease causing rapid and reproducible larval mortality with pathologies similar to those seen during disease epizootics. Vectored challenge via the aquaculture live feed organism Artemia (brine shrimp) caused consistent cumulative mortality rates of 84 to 89% after 72 h, in contrast to variable mortality rates seen after immersion challenge. Histopathological examination of vector-challenged phyllosomas revealed bacterial proliferation in the midgut gland (hepatopancreas) concomitant with epithelial cell necrosis. A fluorescent-protein-labeled V. owensii DY05 transconjugant showed dispersal of single cells in the foregut and hepatopancreas 6 h postexposure, leading to colonization of the entire hepatopancreas within 18 h and eventually systemic infection. V. owensii DY05 is a marine enteropathogen highly virulent to P. ornatus phyllosoma that uses vector-mediated transmission and release from host association to a planktonic existence to perpetuate transfer. This understanding of the infection process will improve targeted biocontrol strategies and enhance the prospects of commercially viable larviculture for this valuable spiny lobster species. Major steps toward the commercialization of closed life cycle aquaculture production of the ornate spiny lobster (Panulirus ornatus) have been reported recently (36), yet nutritional deficits (41) and mortality caused by bacterial disease (5) remain major constraints to hatchery productivity. Mass mortalities of larvae (phyllosomas) are often associated with enteric vibriosis, an infection of the midgut gland (hepatopancreas) caused by Vibrio species (6, 52).Members of the genus Vibrio are natural marine inhabitants, playing important roles in nutrient cycling and forming associations with zooplankton (49). Microhabitat preferences and ecological selection may be key factors in the speciation of vibrios (20), and intensive aquaculture systems are thought to select for bacterial virulence, including traits that enhance infectivity and transmission (29,33). Accordingly, many Vibrio species are pathogenic to cultured crustacean zooplanktonic larval forms, including the three closely related species Vibrio harveyi (32, 35), V. campbellii (17, 44), and the recently described V. owensii (8).It is of paramount importance to the development of efficient disease management strategies that pathogens be identified by using experimental infection models that provide information on infection routes and infection dynamics (38). Recently, microorganisms engineered to express fluorescent proteins (FP) have significantly increased the understanding of invasive pathways and infection dynamics of pathogens, including V. anguillarum, Aeromonas hydrophila, and Edwardsiella tarda in fish models (10, 24, 30) and V. harveyi in abalone (50). Panulirus sp. p...

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Section: Discussionsupporting

confidence: 60%

Pathogenicity and Infection Cycle of Vibrio owensii in Larviculture of the Ornate Spiny Lobster (Panulirus ornatus)

Goulden

Hall

Bourne

et al. 2012

Appl Environ Microbiol

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“…Therefore, classification of Vibrio species has to rely on more discriminative methods such as MLSA, which analyzes a set of housekeeping genes such as rpoA, ftsZ, mreB, gapA, pyrH, topA, recA, rpoD, gyrB, rctB, and toxR (Thompson et al ; Sawabe et al ; Pascual et al ; Hoffmann et al ; Chonchanok et al ). Recently, Yue et al () reported that MLSA of four housekeeping genes (gapA, ftsZ, mreB, and topA) could provide enough evidence for resolving closely related Vibrio species. Accordingly, in addition to the analysis of 16S rRNA, gapA, ftsZ, mreB, and topA genes were sequenced and used to identify XA1.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Molecular Identification of Vibrio natriegens from Diseased Portunus trituberculatus in China

Zhang

Yan

et al. 2016

J World Aquaculture Soc

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In July 2010, heavy mortalities were seen in cultured swimming crabs, Portunus trituberculatus, from Jiangsu Province in China. Two isolates, XA1 and XA2, were isolated from cardiac muscle and somatic muscle of the moribund crabs on ZoBell's marine 2216E agar and were confirmed as the causative agent for the mass mortalities seen on that farm. The morphological and physiological characteristics as well as the biochemical tests of the two isolates were identical and were similar to Vibrio natriegens. Both were gram-negative, oxidase positive, produced acid when fermenting glucose, and failed to grow in the media containing 1% NaCl. Furthermore, the sequencing of the 16S rRNA, gapA, ftsZ, mreB, and topA genes revealed that the two isolates were identical and had a 0.99 homology coefficient with V. natriegens. The Neighbor-Joining tree based on the concatenated sequences of four housekeeping genes (gapA, ftsZ, mreB, and topA) strongly supported that XA1 was most closely related to V. natriegens within the Vibrio harveyi-related clade. In addition, pathogenicity testing using immersion challenge confirmed that strain XA1 was virulent to juvenile crabs, and the LD50 value was 1.09 × 10 6 . This is the first report on V. natriegens as a virulent pathogen for Portunus trituberculatus.

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“…However, three other dominant pathogenic Vibrio, including V. harveyi, V. algolyticus, and V. parahaemolyticus, were also detected in this study. V. parahaemolyticus is the major Vibrio species causing human illness and has emerged as a severe global threat to human health through the consumption of raw or undercooked seafood (Yue et al, 2010;Yue et al, 2011;Silva et al, 2018;Park et al, 2019). V. harveyi is reported to be the primary pathogen of cultured prawns (Stalin and Srinivasan, 2016).…”

Section: Discussionmentioning

confidence: 99%

Prevalence, antibiotic and heavy metal resistance of Vibrio spp. isolated from the clam Meretrix meretrix at different ages in Geligang, Liaohe estuary in China

Zhang

Tian

et al. 2022

Front. Mar. Sci.

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Vibrio as one of the main pathogens of shellfish diseases can cause serious human seafoodborne gastroenteritis and even death. In this study, we analyzed the bacterial communities from the clam, and compared the resistance phenotypes and genotypes of Vibrio spp. from Meretrix meretrix at different growth stages. High-throughput sequencing analysis revealed the predominance of Proteobacteria (50%) in the bacterial community and Vibrio was one of the dominant genera in the clam hepatopancreas in the summer. Vibrio abundance in Meretrix meretrix positively correlated with the water temperature (p<0.05). A total of 73 Vibrio isolates from Meretrix meretrix were classified into 19 species and the dominant strains included V. mediterranei (19%) and V. harveyi (11%), V. algolyticus (10%), and V. parahaemolyticus (8%). The species and abundance of Vibrio spp. were the highest in the 3-year-old of Meretrix meretrix compared with clams of other ages in the summer. Among the 73 isolates, 68 Vibrio strains were resistant to other 15 antibiotics except for sulfamethoxazole-trimethoprim with 57 resistant phenotypes. The most prevalent resistance was toward clindamycin (76%), followed by amikacin (63%), ampicillin (62%), rifampicin (62%), vancomycin (57%), and amoxicillin (50%). The ARI values of Vibrio spp. in different ages ranged from 0.13 to 0.18, and ARI values of 3-year-old (ARI=0.18) clams are higher than that of other ages clam. Approximately 72% of the resistant isolates showed multidrug-resistant phenotypes with maximum resistance to 15 antibiotics. Tolerance to heavy metals including Cd, Zn, and Cu was detected in the majority of antibiotic resistant isolates. In addition to the co-resistance to the same class of antibiotics, resistance to cephalosporin (CFP, CEP, CZ) were significantly correlated with penicillins (AMP, AMC) (p< 0.01), tetracycline (p < 0.001), sulfanilamide (SXT) (p< 0.01) and quinolone (CIP) (p< 0.01). The heavy metal resistance genes copB and nccA were significantly correlated with the clindamycin resistance phenotype (p<0.01). This study revealed that the habitat of Meretrix meretrix is in low exposure to antibiotics, and a link between heavy metal resistance genes and antibiotic resistance.

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Isolation and characterization of a virulent Vibrio sp. bacterium from clams (Meretrix meretrix) with mass mortality

Cited by 20 publications

References 44 publications

Pathogenicity and Infection Cycle of Vibrio owensii in Larviculture of the Ornate Spiny Lobster (Panulirus ornatus)

Pathogenicity and Infection Cycle of Vibrio owensii in Larviculture of the Ornate Spiny Lobster (Panulirus ornatus)

Isolation and Molecular Identification of Vibrio natriegens from Diseased Portunus trituberculatus in China

Prevalence, antibiotic and heavy metal resistance of Vibrio spp. isolated from the clam Meretrix meretrix at different ages in Geligang, Liaohe estuary in China

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