Isolation and characterization of a Rhodococcus strain with phenol-degrading ability and its potential use for tannery effluent biotreatment

Paisio, Cintia Elizabeth; Talano, Melina A.; González, Pablo J.; Busto, Víctor Daniel; Talou, Julián Rodríguez; Agostini, Elizabeth

doi:10.1007/s11356-012-0870-8

Cited by 43 publications

(18 citation statements)

References 34 publications

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“…Since the bioremediation efficiency in 3:1 diluted tannery effluent was less than 1:1 diluted effluent, the latter was preferred for further experiments on tannery effluent biotreatment.An appreciable extent of growth, decolorization, dechlorination, and Cr 6+ removal in diluted as compared to undiluted effluent could be due to reduced toxicity of metals and organic pollutants upon dilution. Contrary to our findings, Paisio et al [7] observed highest growth of Rhodococcus sp. CS1 in pure tannery wastewater as compared to diluted (25, 50 and 75%) effluent.…”

Section: Bioremediation Of Diluted Tannery Effluentcontrasting

confidence: 99%

“…Cr 6+ is highly soluble, mutagenic and carcinogenic whereas, Cr 3+ is less soluble and hence less toxic [5]. In addition, it is well known that leather industries generate complex wastewater containing not only chromium but also other toxic compounds like phenol and its derivatives [6,7]. Phenolic compounds also contribute to offflavor problems in drinking and food processing waters.…”

Section: +mentioning

confidence: 99%

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Microbial Treatment of Tannery Effluent by Augmenting Psychrotrophic Pseudomonas Putida Isolate

Garg¹,

Garg²,

Tripathi³

et al. 2018

EPP

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Abstract:In this study, Pseudomonas putida, a psychrotrophic isolate, has been employed for biological treatment of tannery effluent. The physicochemical parameters of tannery effluent were analysed. The dilution (1:1) of effluent, its supplementation with nutrients (e.g., glucose, yeast extract, peptone, each at 0.5%, w/v) and augmentation with P. putida test strain was done for bioremediation studies at natural pH 8.0. The efficient growth (OD 0.582), Cr 6+ removal (61.0%), decolorization (60.1%) and dechlorination (87.5%) were observed in diluted effluent during 96-120 h incubation in shake flask trial. Box-Behnkem design suggested simultaneous dechlorination (95.8%) and Cr 6+ removal (63.5%) in nutrients supplemented plus P. putida augmented only within 72 h incubation. The efficiency of bioremediation further enhanced in bioreactor. A direct correlation existed between chromate reductase activity and distribution of reduced chromium in different cell fractions and culture supernatant. Further enhanced dechlorination (68.2%) by 14.7% and Cr 6+ removal (72.7%) by 6.5% employing immobilized co-culture (P. putida and Bacillus cereus) as compared to bioremediation by single immobilized P. putida biomass. High extent of effluent bioremediation by immobilized biomass offer an attractive future of P. putida biomass for eco-friendly in situ bioremediation of tannery wastewater.

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Section: Bioremediation Of Diluted Tannery Effluentcontrasting

confidence: 99%

Section: +mentioning

confidence: 99%

Microbial Treatment of Tannery Effluent by Augmenting Psychrotrophic Pseudomonas Putida Isolate

Garg¹,

Garg²,

Tripathi³

et al. 2018

EPP

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“…B9. A similar microbial removal of phenol from tannery wastewater using Rhodococcus strain has been reported by Paisio et al [32] The strain was also active in decontaminating phenol from the wastewater at high phenol concentrations and showed 100% phenol removal but with lower degradation rates. In the presence of higher phenol content of 1.0 and 2.0 mM, strain B9 resulted in complete removal of phenol content at 72 and 96 h, respectively (Figure 4 effluents by pure culture of Acinetobacter sp.…”

Section: Application Of Strain B9 In Dephenolisation Of Industrial Wasupporting

confidence: 78%

Assessment of phenol-degrading ability ofAcinetobactersp. B9 for its application in bioremediation of phenol-contaminated industrial effluents

Bhattacharya

Gupta

Kaur

et al. 2015

Chemistry and Ecology

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Successful bioremediation of a phenol-contaminated environment requires application of those microbial strains that have acquired phenol tolerance and phenol-degrading abilities. A newly isolated strain B9 of Acinetobacter sp. was adapted to a high phenol concentration by growing sequentially from low-to highstrength phenol. The acclimatised strain was able to grow and completely degrade up to 14 mM of phenol in 136 h. The degradation rates were found to increase with an increase in the phenol concentration from 2.0 to 7.5 mM. The strain preferred neutral to alkaline pH range for growth and phenol degradation, with the optimum being pH 8.0. The optimum temperature for phenol degradation was found to be in the range of 30-35°C. Transmission electron micrographs showed a disorganised and convoluted cell membrane in the case of phenol-stressed cells, showing a major effect of phenol on the membrane. Enzymatic and gas chromatography-mass spectrometry studies show the presence of an ortho-cleavage pathway for phenol degradation. Efficient phenol degradation was observed even in the presence of pyridine and heavy metals as co-toxicants showing the potential of strain in bioremediation of industrial wastes. Application of strain B9 to real tannery wastewater showed 100% removal of initial 0.5 mM phenol within 48 h of treatment.

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“…2 industrial wastewater was also found to be decolorized after treatment. Similar microbial treatment of tannery wastewater for the simultaneous removal of chromate and phenolics has been reported by Srivastava et al (2007) using consortia of microbes, whereas individual removal of either phenol or Cr (VI) from tannery wastewater has been demonstrated by Paisio et al (2012) using Rhodococcus strain and Panda and Sarkar (2012) using Enterobacter aerogenes T2, respectively. …”

Section: Simultaneous Remediation Of Phenol and Cr (Vi) From Industrisupporting

confidence: 55%

Simultaneous Bioremediation of Phenol and Cr (VI) from Tannery Wastewater Using Bacterial Consortium

Bhattacharya

Gupta

Kaur

et al. 2015

Int J Appl Sci Biotechnol

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In the present study a consortium of four naturally isolated bacterial strains was evaluated as remediation tool for simultaneous removal ofphenol and Cr (VI) from tannery effluent. Application of bacterial consortia to effluent (pH 4.6) resulted in 100 and 78% removal of initial 47mg L-1 phenol and 16 mg L-1 Cr (VI), respectively at 96 h of treatment. The consortium was also active in removal of contaminants with lowerremoval rate in presence of extraneous higher concentrations of both phenol and Cr (VI). Treatment in static mode also resulted in removal ofpollutants, however with increase in agitation speed simultaneous reduction of contaminants becomes faster. Overall it can be inferred fromthe study that the above formulated bacterial consortium could effectively be used for treatment of phenol and Cr (VI) laden tannery and otherindustrial effluents.DOI: http://dx.doi.org/10.3126/ijasbt.v3i1.11889 Int J Appl Sci Biotechnol, Vol. 3(1): 50-55

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Isolation and characterization of a Rhodococcus strain with phenol-degrading ability and its potential use for tannery effluent biotreatment

Abstract: Based on these results, Rhodococcus sp. CS1 could be an appropriate microorganism for bioremediation of tannery effluents or other phenol-containing wastewaters.

Cited by 43 publications

References 34 publications

Microbial Treatment of Tannery Effluent by Augmenting Psychrotrophic Pseudomonas Putida Isolate

Microbial Treatment of Tannery Effluent by Augmenting Psychrotrophic Pseudomonas Putida Isolate

Assessment of phenol-degrading ability ofAcinetobactersp. B9 for its application in bioremediation of phenol-contaminated industrial effluents

Simultaneous Bioremediation of Phenol and Cr (VI) from Tannery Wastewater Using Bacterial Consortium

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