Isolation and characterization of a novel lytic bacteriophage vB_Efm_LG62 infecting Enterococcus faecium

Qu, Qingsong; Chen, Tao; He, Ping; Geng, Huaixin; Zeng, Peibin; Luan, Guangxin

doi:10.1007/s11262-023-02016-9

Cited by 7 publications

(6 citation statements)

References 47 publications

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“…A total of 71 and 72 ORFs were predicted in dArtagnan and Aramis genomes, respectively. Both phages are related (61% to 69% nucleotide identity along the entire genomes) to Enterococcus phages vB_Efm_LG62 ( 56 ), IME-EFm1 ( 57 ), and -EFm5 ( 58 ) ( Fig. 3B and Fig.…”

Section: Resultsmentioning

confidence: 99%

The virtue of training: extending phage host spectra against vancomycin-resistant Enterococcus faecium strains using the Appelmans method

Lossouarn,

Beurrier,

Bouteau

et al. 2024

Antimicrob Agents Chemother

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Phage therapy has (re)emerged as a serious possibility for combating multidrug-resistant bacterial infections, including those caused by vancomycin-resistant Enterococcus faecium strains. These opportunistic pathogens belong to a specific clonal complex 17, against which relatively few phages have been screened. We isolated a collection of 21 virulent phages growing on these vancomycin-resistant isolates. Each of these phages harbored a typical narrow plaquing host range, lysing at most 5 strains and covering together 10 strains of our panel of 14 clinical isolates. To enlarge the host spectrum of our phages, the Appelmans protocol was used. We mixed four out of our most complementary phages in a cocktail that we iteratively grew on eight naive strains from our panel, of which six were initially refractory to at least three of the combined phages. Fifteen successive passages permitted to significantly improve the lytic activity of the cocktail, from which phages with extended host ranges within the E. faecium species could be isolated. A single evolved phage able to kill up to 10 of the 14 initial E. faecium strains was obtained, and it barely infected nearby species. All evolved phages had acquired point mutations or a recombination event in the tail fiber genetic region, suggesting these genes might have driven phage evolution by contributing to their extended host spectra.

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Section: Resultsmentioning

confidence: 99%

The virtue of training: extending phage host spectra against vancomycin-resistant Enterococcus faecium strains using the Appelmans method

Lossouarn,

Beurrier,

Bouteau

et al. 2024

Antimicrob Agents Chemother

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“…Sequencing data were assembled de novo using Metaviral SPAdes v.3.15.3 (Antipov et al, 2020). The complete genome of the phage was annotated and analyzed using a variety of bioinformatics tools as described below (Qu et al, 2023). The complete genome of the phage was predicted by Rapid Annotation using the Subsystem Technology (RAST) server (http://rast.nmpdr.org/) (Overbeek et al, 2014).…”

Section: Phage Sequencing and Genome Analysismentioning

confidence: 99%

“…The circular genome of the phage was visualized using the CGView server database (http://wishart.biology.ualberta.ca/cgview/) (Stothard and Wishart, 2005) and annotated using Artemis Release_18.2.0 (Carver et al, 2012). To analyze the evolutionary relationship between phage Abp4 and other Acinetobacter phages (Supplementary Table S3), phylogenetic analysis was generated by the MEGA_11.0 software based on the maximum-likelihood (ML) algorithm with 1000 bootstrap replicates (Qu et al, 2023), and the multiple sequence alignment was performed by MUSCLE (Edgar, 2004). Genome comparative analysis was generated using EasyFigure_2.2.3 (Sullivan et al, 2011) software to reveal how dissimilar these phages are in terms of genomic architecture.…”

Section: Phage Sequencing and Genome Analysismentioning

confidence: 99%

Host range expansion of Acinetobacter phage vB_Ab4_Hep4 driven by a spontaneous tail tubular mutation

He,

Cao,

et al. 2024

Front. Cell. Infect. Microbiol.

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Bacteriophages (phages) represent promising alternative treatments against multidrug-resistant Acinetobacter baumannii (MDRAB) infections. The application of phages as antibacterial agents is limited by their generally narrow host ranges, so changing or expanding the host ranges of phages is beneficial for phage therapy. Multiple studies have identified that phage tail fiber protein mediates the recognition and binding to the host as receptor binding protein in phage infection. However, the tail tubular-dependent host specificity of phages has not been studied well. In this study, we isolated and characterized a novel lytic phage, vB_Ab4_Hep4, specifically infecting MDRAB strains. Meanwhile, we identified a spontaneous mutant of the phage, vB_Ab4_Hep4-M, which revealed an expanded host range compared to the wild-type phage. A single mutation of G to C was detected in the gene encoding the phage tail tubular protein B and thus resulted in an aspartate to histidine change. We further demonstrated that the host range expansion of the phage mutant is driven by the spontaneous mutation of guanine to cytosine using expressed tail tubular protein B. Moreover, we established that the bacterial capsule is the receptor for phage Abp4 and Abp4-M by identifying mutant genes in phage-resistant strains. In conclusion, our study provided a detailed description of phage vB_Ab4_Hep4 and revealed the tail tubular-dependent host specificity in A. baumannii phages, which may provide new insights into extending the host ranges of phages by gene-modifying tail tubular proteins.

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“…A typical phage particle structure consists of a three-dimensional shape made up of an icosahedral protein capsid with or without a tail and a filament [105]. Phage particles can range in size from 24 to 200 nm [106,107], with the largest being the T4 phage, which infects E. coli and is 200 nm long and 80-100 nm wide [108]. The physical properties of phages specific to E. coli O157 can vary depending on their family.…”

Section: Characteristics Of E Coli O157:h7 Phagesmentioning

confidence: 99%

Safety Properties of Escherichia coli O157:H7 Specific Bacteriophages: Recent Advances for Food Safety

Oluwarinde,

Ajose,

Abolarinwa

et al. 2023

Foods

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Shiga-toxin-producing Escherichia coli (STEC) is typically detected on food products mainly due to cross-contamination with faecal matter. The serotype O157:H7 has been of major public health concern due to the severity of illness caused, prevalence, and management. In the food chain, the main methods of controlling contamination by foodborne pathogens often involve the application of antimicrobial agents, which are now becoming less efficient. There is a growing need for the development of new approaches to combat these pathogens, especially those that harbour antimicrobial resistant and virulent determinants. Strategies to also limit their presence on food contact surfaces and food matrices are needed to prevent their transmission. Recent studies have revealed that bacteriophages are useful non-antibiotic options for biocontrol of E. coli O157:H7 in both animals and humans. Phage biocontrol can significantly reduce E. coli O157:H7, thereby improving food safety. However, before being certified as potential biocontrol agents, the safety of the phage candidates must be resolved to satisfy regulatory standards, particularly regarding phage resistance, antigenic properties, and toxigenic properties. In this review, we provide a general description of the main virulence elements of E. coli O157:H7 and present detailed reports that support the proposals that phages infecting E. coli O157:H7 are potential biocontrol agents. This paper also outlines the mechanism of E. coli O157:H7 resistance to phages and the safety concerns associated with the use of phages as a biocontrol.

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Isolation and characterization of a novel lytic bacteriophage vB_Efm_LG62 infecting Enterococcus faecium

Cited by 7 publications

References 47 publications

The virtue of training: extending phage host spectra against vancomycin-resistant Enterococcus faecium strains using the Appelmans method

The virtue of training: extending phage host spectra against vancomycin-resistant Enterococcus faecium strains using the Appelmans method

Host range expansion of Acinetobacter phage vB_Ab4_Hep4 driven by a spontaneous tail tubular mutation

Safety Properties of Escherichia coli O157:H7 Specific Bacteriophages: Recent Advances for Food Safety

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