Isolation and Characterization of a Lactose-Binding Lectin from Ocimum sanctum

Vemuri, Praveen Kumar; Talluri, Bhavana; Sharma, Ananya; Akkala, Geethika; Bodiga, Vijaya Lakshmi

doi:10.7324/japs.2015.501019

Cited by 10 publications

(9 citation statements)

References 17 publications

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“…This lysate is subjected for dialysis against phosphate-buffered saline (PBS) buffer for 4 h to remove impurities and made as smoothie. [11]…”

Section: Extraction and Purification Of Oyster Mushroommentioning

confidence: 99%

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2022

AJP

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Background: Pleurotus ostreatus, the oyster mushroom, is primarily an edible mushroom with high nutritional value and biomedical relevance, as it contains numerous bioactive components that generate the development of therapeutic effects. Aim: The purpose of this study is to determine the phytopharmacological effects of P. ostreatus. Material and Methods: To depict the medicinal properties of oyster mushroom, FTIR, HPLC, GC-MS, anticancer and antimicrobial activities were conducted. Results: The presence of 12 bioactive compounds was revealed using gas chromatography-mass spectrometry, and components in the extract were identified and quantified using high pressure liquid chromatography. The extract's anticancer efficacy against HeLa and MCF-7 at 25 µg/ ml and exhibits strong cytotoxicity activity of 6.8 µg/mL and 17.5 µg/mL, respectively. Antimicrobial efficacy of P. ostreatus aqueous extract against Bacillus megaterium and Bacillus amyloliquefaciens seems promising. A significant inhibitory zone was discovered against B. megaterium at 90 µg/ml of sample. Conclusion: Our primary data from this research work can help in the detection of bioactive component's, the determination of their efficacy through in vivo research, and the demonstration of their safety and efficacy in clinical trials.

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“…This lysate is subjected for dialysis against phosphate-buffered saline (PBS) buffer for 4 h to remove impurities and made as smoothie. [11]…”

Section: Extraction and Purification Of Oyster Mushroommentioning

confidence: 99%

Untitled

2022

AJP

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“…All soil samples as listed in Table 1 are collected from different areas located in loco sheds, Vijayawada City, Andhra Pradesh, India, and they were sealed carefully with zip lock covers to avoid contaminants and dust. [9] ORIGINAL ARTICLE…”

Section: Collection and Samples Processingmentioning

confidence: 99%

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2021

AJP

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“…They are used to detect specific red cell antigens, to activate various types of lymphocytes, and to resolve poly agglutination issues [3]. Lectins are powerful tools for recognizing a diverse range of oligosaccharides, and they have been widely used in many fields of cell biology, biochemistry, and food technology [4][5][6][7][8]. These are glycoprotein domains with highly specific pockets for their counter sugar moieties of polysaccharides, glycolipids, glycoproteins, proteoglycans, and peptidoglycans found on the exterior walls or membranes of both vertebrate and invertebrate cells and microorganisms [9].…”

Section: Introductionmentioning

confidence: 99%

Studies on Lectin Mediated Agglutination Reaction on Red Blood Cell Surface Antigens Using Hot and Cold Water Plant Extracts

Velagapudi

Nannapaneni

Alampally

et al. 2021

JPRI

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Lectin has various physiological roles in cell agglutination, based on their carbohydrate-binding properties, plant lectins are widely used for the detection, segregation, and characterization of glycoconjugates. Rhesus (Rh) factor is a protein that is inherited and found on the surface of red blood cells. If the surface protein is present, the RBC is Rh positive; otherwise, it is Rh-negative in nature. In this paper, we use agglutination reactions to investigate the effect of different cold and hot water extracted plants on RBC antigens as an alternative to commercial monoclonal antibodies. Extensive research on the sequence homology and 3-D structure of various plant lectins suggests that they have been conserved throughout evolution and may play important physiological roles that are still unknown.

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Isolation and Characterization of a Lactose-Binding Lectin from Ocimum sanctum

Cited by 10 publications

References 17 publications

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Studies on Lectin Mediated Agglutination Reaction on Red Blood Cell Surface Antigens Using Hot and Cold Water Plant Extracts

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