Isolation and characterization of a dual function protein from Allium sativum bulbs which exhibits proteolytic and hemagglutinating activities

Parisi, Mónica Graciela; Moreno, Sílvia; Fernandez, Gina E.

doi:10.1016/j.plaphy.2007.11.003

Cited by 17 publications

(12 citation statements)

References 45 publications

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“…The premise that the sugar binding and PPO activity exist on different loci is reflected by the inhibition studies (Table 6). Parisi et al [33] also report an additional cysteine-peptidase of the well known mannose specific lectin from garlic (Allium sativum) bulbs.…”

Section: Discussionmentioning

confidence: 99%

The unique enzymatic function of field bean (Dolichos lablab) d-galactose specific lectin: a polyphenol oxidase

et al. 2008

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The polyphenol oxidase (PPO) of field bean (Dolichos lablab) is a tetramer made up of two subunits of mass 29,000 and 31,000 Da. The amino acid sequence of the tryptic peptides showed approximately 90% sequence identity to the D: -galactose specific legume lectins. The haemagglutinating activity of a pure and homogenous preparation of PPO measured using human erythrocytes was 1261 HAU mg(-1) protein and was inhibited by D: -galactose. Purification by galactose-sepharose chromatography also indicated that the PPO and haemagglutinating activities were associated with a single protein. Crude extracts of other legumes did not exhibit PPO activity, yet cross reacted with anti-PPO antibodies. This dual function protein with PPO and haemagglutinating activity is unique to field bean. The two activities are independent of each other occurring at different loci on the protein. These observations further evidence and strengthen the assumption that galactose specific legume lectins have enzymatic function. Both PPO and lectins are proteins that play a vital role in the defense mechanism of plants. The complementarity of these two simultaneous and independent powerful defense mechanisms exhibited by a single protein renders it a candidate gene for the development of inbuilt plant protection.

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Section: Discussionmentioning

confidence: 99%

The unique enzymatic function of field bean (Dolichos lablab) d-galactose specific lectin: a polyphenol oxidase

et al. 2008

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“…For example, Parisi et al (2008) reported that a dual function protein (25-26 kDa) was isolated from A. sativum bulbs which exhibited proteolytic and hemagglutinating activities. Hadji et al (2007) demonstrated that crude garlic extract contained one Mn-superoxide dismutase designated as SOD1 and two Cu, Zn superoxide dismutases as SOD2 and SOD3.…”

Section: Discussionmentioning

confidence: 99%

Changes in antioxidant capacity, levels of soluble sugar, total polyphenol, organosulfur compound and constituents in garlic clove during storage

Fei

et al. 2015

Industrial Crops and Products

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“…Some studies have suggested that either carbohydrate-binding activity or proteolytic activity as an important parameter for the immunogenicity of proteins. It is of interest to note here that a garlic protein with sequence similarity to the lectin ASA II has been shown to possess both hemagglutination and cysteinyl protease activity [37]. Our results clearly show that both ASA I and ASA II, upon oral administration, produce a remarkable humoral immune response compared to control.…”

Section: Discussionmentioning

confidence: 53%

Dietary garlic (Allium sativum) lectins, ASA I and ASA II, are highly stable and immunogenic

Clement

Venkatesh

2010

International Immunopharmacology

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Isolation and characterization of a dual function protein from Allium sativum bulbs which exhibits proteolytic and hemagglutinating activities

Cited by 17 publications

References 45 publications

The unique enzymatic function of field bean (Dolichos lablab) d-galactose specific lectin: a polyphenol oxidase

The unique enzymatic function of field bean (Dolichos lablab) d-galactose specific lectin: a polyphenol oxidase

Changes in antioxidant capacity, levels of soluble sugar, total polyphenol, organosulfur compound and constituents in garlic clove during storage

Dietary garlic (Allium sativum) lectins, ASA I and ASA II, are highly stable and immunogenic

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