Isolation and characterization of a first set of polymorphic microsatellite markers in saffron, <i>Crocus sativus</i> (Iridaceae)

Nemati, Zahra; Zeinalabedini, Mehrshad; Mardi, Mohsen; Pirseyediand, Seyed M.; Marashi, Hassan; Nekoui, S. M. Khayam

doi:10.3732/ajb.1100531

Cited by 29 publications

(18 citation statements)

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“…The average PIC value was 0.38 ranging from 0.1 to 0.54 (Table 2). Nemati et al (2012) observed that a total of 27 alleles with a mean of 2.60 per locus and the mean of PIC across all loci varied from 0.1 to 0.54 with an average of 0.34 in their SSR analysis, which is apparently compatible with our present results. Moreover, our results showed a new set of 12 polymorphic microsatellite loci out of 15 monomorphic microSaffron (Crocus sativus L.), a monomorphic or polymorphic species?…”

Section: Discussionsupporting

confidence: 92%

“…sativus as cultivated crocus is a sterile species with distinct morphological and growing characterisSaffron (Crocus sativus L.), a monomorphic or polymorphic species? 757 (Siracusa et al, 2012;Erol et al, 2014) and SSR (Rubio-Moraga et al, 2009;Nemati et al, 2012), the actual genetic diversity present in C. sativus is still an open question. In this study, 27 microsatellite markers were tested to measure level of polymorphism and investigated the genetic relationship and structure among Iranian Crocus ecotypes.…”

Section: Discussionmentioning

confidence: 99%

“…Studying SSR markers, Rubio-Moraga et al (2009) evaluated 43 saffron isolates by 15 microsatellite primers, none of which was polymorphic in the population under study. On the contrary, Nemati et al (2012) detected a good level of polymorphism by 12 microsatellite markers within 50 Iranian individuals of Crocus sativus. The purpose of this study was to investigate the spectrum of genetic diversity within Iranian Crocus spp., to reveal the phylogenetic relationships of 67 Crocus collected from different sites of Iran using 27 microsatellite markers, to propose a strategy for broadening the genetic base for future breeding of this valuable crop.…”

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confidence: 86%

“…The quality of the extracted DNA was verified on a 1% agarose gel and the amount of total genomic DNA obtained 754 Z. Nemati et al / Span J Agric Res (2014) Nemati et al (2012). Each 25 µL PCR reaction contained 2.5 µL of 1x PCR buffer, 2 µL of 25 mM MgCl 2 , 1.5 µL of 10 mM dNTPs, 1 µL of each primer (10 pmol µL -1 ), 0.2 µL of Taq DNA polymerase (5 U µL -1 ) (Fermentas), 14.8 µL of double distilled water, and 2 µL of extracted DNA (ca.…”

Section: Molecular Analysismentioning

confidence: 99%

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Saffron (Crocus sativus L.), a monomorphic or polymorphic species?

Nemati

Mardi

Majidian

et al. 2014

Span. j. agric. res.

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Saffron (Crocus sativus L.) which contains exceptional anti-cancer properties is presently the world's most expensive spice. Iran is known as the original habitat of Crocus L. and a significant source of high-quality cultivated saffron production and export. Considering the importance of this species, we used 27 microsatellite markers to assess molecular variability and discriminating capacity of markers regarding their effectiveness in establishing genetic relationships in Iranian Crocus ecotypes. Thirty eight Iranian cultivated saffron ecotypes and 29 wild allies were evaluated in this research. The results from molecular analyses, including a molecular phylogenetic network and RB analysis, revealed two major groups and five subgroups, regardless of their geographical origins. Also, the results showed a clear distinction between C. sativus and other species of Crocus genus, taking into account their close relationship with C. speciosus and C. hausknechtii, which are assumed to be the two closest relatives of Iranian cultivated saffron among species studied. In this paper, we observed for the first time extensive genetic diversity among Iranian C. sativus despite their asexual reproduction. Considering suitable climatic conditions in Iran for cultivating saffron and the country’s leading high-quality production of Crocus sativus worldwide, studies on great genetic variability among Iranian C. sativus ecotypes as well as wild relatives native to Iran will further highlight the value of this crop. In addition, our results provide valuable information for genetic improvement, reduction of strong genetic erosion, and conservation of costly heritable resources of C. sativus in future breeding programs.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 86%

Section: Molecular Analysismentioning

confidence: 99%

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Saffron (Crocus sativus L.), a monomorphic or polymorphic species?

Nemati

Mardi

Majidian

et al. 2014

Span. j. agric. res.

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“…A further six primer pairs were designed for simple sequence repeats from sequences of C. sativus registered in GenBank (NCBI) (Nemati et al 2012). Primer sequences and individual annealing temperatures for each primer pair used are shown in Table 2.…”

Section: Ssr-primer-designmentioning

confidence: 99%

Large intraspecific genetic variation within the Saffron-Crocus group (Crocus L., Series Crocus; Iridaceae)

et al. 2014

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Series Crocus comprises ten autumn-flowering species, including the cultivated Crocus sativus, SaffronCrocus. Interspecific genetic variation was examined in all species of the series, except for C. naqabensis. Intraspecific genetic and morphological variation was considered in the three Greek endemics, C. cartwrightianus, C. hadriaticus and C. oreocreticus. Genetic variation was evaluated based on amplified fragment length polymorphism and simple sequence repeats analyses, including 94 and 233 specimens, respectively. Analysis of molecular variance demonstrated significant genetic variation within populations, compared with low genetic variation between populations suggesting substantial gene flow between populations. In Neighbour-Net analysis, C. hadriaticus samples from mainland Greece were separated from Peloponnesian samples; C. cartwrightianus, C. hadriaticus and C. oreocreticus generally were grouped with C. sativus samples. Pollination and maintenance of genetic variation are discussed. The large intraspecific variation found within the three specifically studied species reflects dynamic population structures with potential to meet future ecological fluctuations. It emphasises a large gene pool which should be considered by including a larger number of accessions in genetic diversity studies.

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Distribution, Diversity, Conservation and Utilization of Threatened Medicinal Plants

Rajasekharan

Wani

2020

Conservation and Utilization of Threatened Medicinal Plants

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Rich biodiversity of India is under severe threat owing to habitat destruction, degradation, fragmentation, and overexploitation of resources. According to the Red List of threatened plants, 44 plant species are critically endangered, 113 endangered, and 87 vulnerable (IUCN, 2000). Widespread losses of plant species and varieties are eroding the foundation of agricultural productivity and threatening other plant-based products used by billions of people worldwide, as reported in a new study by the World Watch Institute, Washington, and worldwide some 3.5 billion people in developing countries rely on plant-based medicine for primary health care. Loss of habitat, pressure from nonactive species, and over harvesting have put one out of every eight plant species at risk of extinction, according to the world conservation union. Many medicinal plants are also in trouble from over harvesting and destruction of habitat. Since less than 1 percent of all species have been screened for bioactive compounds, every loss of a unique habitat and its species is potentially a loss of future drugs and medicines.

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Isolation and characterization of a first set of polymorphic microsatellite markers in saffron, Crocus sativus (Iridaceae)

Abstract: The microsatellite markers reported here will be useful for evaluating genetic diversity and will likely serve as an ideal resource for use in marker-assisted breeding programs, germplasm analysis, and varietal identification.

Cited by 29 publications

References 10 publications

Saffron (Crocus sativus L.), a monomorphic or polymorphic species?

Saffron (Crocus sativus L.), a monomorphic or polymorphic species?

Large intraspecific genetic variation within the Saffron-Crocus group (Crocus L., Series Crocus; Iridaceae)

Distribution, Diversity, Conservation and Utilization of Threatened Medicinal Plants

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