Isolation and characterization of a thermophilic Bacillus shackletonii K5 from a biotrickling filter for the production of polyhydroxybutyrate

Strategies for Large-scale Production of Polyhydroxyalkanoates

“…In one of the reports, an isolated thermophilic bacteria Bacillus shackletoni K5 was involved in a batch process, which resulted in a satisfactory biopolymer content of 72.6 % dry cell weight (dcw). 28 A similar result with a biomass concentration of 4.8 g L -1 and a P(3HB) content of 73 % dcw was obtained using an isolated Chelatococcus strain by Ibrahim et al 25 In a recent report, growth-associated and non-nitrogen limited P(3HB) production was reported for Chelatococcus daeguensis TAD1. The former helps to reduce the fermentation time thereby improving the productivity of the process.…”

“…As can be seen from Table 1, poly-3-hydroxybutyrate (P(3HB) remains the most widely investigated PHA by batch fermentation, accounting for one-third of the literature reports. However, quite interestingly, there have been only few reports on the utilization of pure or refined substrates (sugars) for P(3HB) production, [27][28][29][30] while different types of inexpensive carbon sources such as agro-industrial wastes including cane molasses, sugar beet juice, rice straw hydrolysate, grass biomass hydrolysate, plant oils e.g., coconut oil, have been largely investigated. [31][32][33][34][35][36][37][38] Alcaligenes and Bacillus sp.…”

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

“…However, PHA produced by Gram-positives is characterized by low levels of LPS causing immunogenic reactions, making them interesting for biomedical applications. The first report on a thermophilic Gram-positive PHA producer was provided by Liu et al [110], who describe the PHA accumulation by the bacterial isolate K5, which displayed excellent growth and PHB-accumulating capacity. Strain K5 has been isolated from the biofilm of a biotrickling filter designed for the removal of NO x from flue gas of a Chinese coal-fired power plant.…”

Draft Genome Sequence of Bacillus shackletonii LMG 18435 T , Isolated from Volcanic Mossy Soil

“…The type strain LMG 18435 T (=CIP 107762 T ) of Bacillus shackletonii was isolated from mossy soil taken from the eastern lava flow of northern Candlemas Island, South Sandwich archipelago ( 1 ). Recently, the thermophilic strain K5 of B. shackletonii was isolated from a biotrickling filter and appeared to have a strong capacity for producing polyhydroxybutyrate (up to 2.28 g/L) ( 2 ). Because of the application prospects and lack of genomic information of B. shackletonii , its type strain LMG 18435 T was selected as one of the research objects in our genome sequencing project for genomic taxonomy and phylogenomics of Bacillus -like bacteria.…”