Isolation and Characterization of a Diagnostic Polysaccharide Antigen From Larval Echinococcus Multilocularis

Sato, Chiaki; Furuya, Koji

doi:10.7883/yoken1952.47.65

Cited by 17 publications

(11 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Em2 is a T-cell independent antigen, and the corresponding antibody response lacks antibody maturation [27]. Another polysaccharide-containing antigen, antigen C has been isolated and characterized from crude metacestode extract [28]. Similar investigations have yielded the finding of EmP2 [29, 30] and Em492 [31].…”

Section: Role Of Parasite-derived Molecules In the Modulation Of Tmentioning

confidence: 99%

Echinococcus multilocularisand Its Intermediate Host: A Model of Parasite-Host Interplay

Vuitton

Gottstein

2010

Journal of Biomedicine and Biotechnology

140

172

View full text Add to dashboard Cite

Host-parasite interactions in the E. multilocularis-intermediate host model depend on a subtle balance between cellular immunity, which is responsible for host's resistance towards the metacestode, the larval stage of the parasite, and tolerance induction and maintenance. The pathological features of alveolar echinococcosis. the disease caused by E. multilocularis, are related both to parasitic growth and to host's immune response, leading to fibrosis and necrosis, The disease spectrum is clearly dependent on the genetic background of the host as well as on acquired disturbances of Th1-related immunity. The laminated layer of the metacestode, and especially its carbohydrate components, plays a major role in tolerance induction. Th2-type and anti-inflammatory cytokines, IL-10 and TGF-β, as well as nitric oxide, are involved in the maintenance of tolerance and partial inhibition of cytotoxic mechanisms. Results of studies in the experimental mouse model and in patients suggest that immune modulation with cytokines, such as interferon-α, or with specific antigens could be used in the future to treat patients with alveolar echinococcosis and/or to prevent this very severe parasitic disease.

show abstract

Section: Role Of Parasite-derived Molecules In the Modulation Of Tmentioning

confidence: 99%

Echinococcus multilocularisand Its Intermediate Host: A Model of Parasite-Host Interplay

Vuitton

Gottstein

2010

Journal of Biomedicine and Biotechnology

140

172

View full text Add to dashboard Cite

show abstract

“…Another laminated layer‐associated antigen was described by Sato and Furuya (1994), and corresponding MAbs were also generated (Sato et al . 1999).…”

Section: Introductionmentioning

confidence: 99%

Review: Molecular tools for the diagnosis of cystic and alveolar echinococcosis

Siles-Lucas

Gottstein

2001

Tropical Med Int Health

View full text Add to dashboard Cite

SummaryIn the past years, the diagnostic tools applied to identify alveolar (AE) and cystic echinococcosis (CE) in human patients have not only increased in number but also substantially improved in quality. The identi®cation and characterization of species-speci®c parasite proteins/antigens allowed to generate subsequently recombinant or synthetic polypeptide antigens, as well as corresponding monoclonal antibodies. Some of these new tools have already demonstrated operating characteristics superior to conventional tests used for the immunodiagnosis of CE and AE, and thus may be suggested for routine laboratory application. Powerful molecular techniques, such as the polymerase chain reaction (PCR), have been developed and adapted to advance laboratory diagnosis of AE and CE. Detecting minute amounts of parasite DNA and mRNA, not only to identify but also to characterize the biological status of parasite material, thus becomes a complementary method to synergize immunodiagnostic techniques. This review focuses on recent developments of molecular tools, discussing their potential use as a primary or a supporting diagnostic element. We also outline some future developments to be undertaken in the ®eld of molecular diagnosis, linked to clinical and laboratory problems.keywords alveolar echinococcosis, cystic echinococcosis, immunodiagnosis, molecular diagnosis correspondence Dr B. Gottstein,

show abstract

“…The antigen may show serological cross-reactivity in that the parasite has been reported to synthesize closely related molecules (9)(10)(11). The idea stemmed from the present lack of data on CA 19-9 values in serum of such patients.…”

Section: Introductionmentioning

confidence: 99%

Elevated Carbohydrate Antigen 19-9 (CA 19-9) in Patients with Echinococcus Infection

Pfister¹,

Gottstein²,

Kretschmer³

et al. 2001

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

The carbohydrate antigen 19-9 (CA 19-9), a determinant (sialylated lacto-N-fucopentaose 119) of a circulating oligosaccharide antigen, is a frequently used tumor marker. Echinococcus spp. infects humans throughout the world and may be able to synthesize closely related molecules which could interfere with the measurement and interpretation of CA 19-9 concentration. The main objective of the present study was to determine the range of CA 19-9 levels in the sera of patients infected by E. granulosus (cystic hydatide disease; CYSHD) or E. multilocularis (alveolar hydatide disease; ALVHD). Serum samples were collected from patients (aged 10-85 years) over a period of 5 years: from 19 patients with CYSHD and from 20 patients with ALVHD. Infection was confirmed by positive Echinococcus serology and clinical evidence provided by imaging and/or histopathological findings. CA 19-9 was detectable in 13 patients with CYSHD (13.5 +/- 8.5 kU/l) and 13 patients with ALVHD (30.0 +/- 21 kU/l; p < 0.05). Thus ALVHD patients exhibited a significantly higher plasma level of CA 19-9 than CYSHD patients. The serum level of CA 19-9 assessed with an increased cut-off value (> 22 kU/l) was elevated in nine (45%) of 20 ALVHD patients compared to two (11%) of 19 CYSHD patients (p < 0.05). Sera from patients with Echinococcus multilocularis infection contain substances which cross-react with CA 19-9. These substances originate either from the parasite or are synthesized by the host in response to the infection, and possibly bear the Lewis-a antigen or closely related structures which are recognized by anti-CA 19-9 antibodies. Our findings are relevant to the investigation of patients presenting with cystic lesions for which the differential diagnosis includes an infectious or neoplastic origin.

show abstract

Isolation and Characterization of a Diagnostic Polysaccharide Antigen From Larval Echinococcus Multilocularis

Cited by 17 publications

References 7 publications

Echinococcus multilocularisand Its Intermediate Host: A Model of Parasite-Host Interplay

Echinococcus multilocularisand Its Intermediate Host: A Model of Parasite-Host Interplay

Review: Molecular tools for the diagnosis of cystic and alveolar echinococcosis

Elevated Carbohydrate Antigen 19-9 (CA 19-9) in Patients with Echinococcus Infection

Contact Info

Product

Resources

About