Isolation and characterization of abscisic acid‐deficient <i>Arabidopsis</i> mutants at two new loci

Léon‐Kloosterziel, Karen M.; Gil, Marta Álvarez; Ruijs, Gerda J.; Jacobsen, Steven E.; Olszewski, Neil E.; Schwartz, Steven H.; Zeevaart, Jan A. D.; Koornneef, M.

doi:10.1046/j.1365-313x.1996.10040655.x

Cited by 445 publications

(442 citation statements)

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“…Possible mechanisms for ABA modulation of sugar signalling are suggested by the observation that the SNF1-related kinase PKABA1 is induced in barley aleurone layers by ABA, and that its constitutive expression drastically suppressed aamylase expression (Go Â mez-Cadenas et al, 1999). In Arabidopsis, the requirement for GA during seed germination has been used to isolate ABA-de®cient mutants (Le Â on- Kloosterziel et al, 1996) and, consistent with the predictions of our model, abi4 mutants are able to germinate in the presence of the gibberellin biosynthesis inhibitor paclobutrazol (Laby et al, 2000). In the absence of ABA signalling promoting a storage mode, less activation of a mobilization mode by gibberellins may be required.…”

Section: Discussionmentioning

confidence: 99%

Impaired sucrose‐induction mutants reveal the modulation of sugar‐induced starch biosynthetic gene expression by abscisic acid signalling

Rook¹,

Corke²,

Card³

et al. 2001

The Plant Journal

375

351

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SummaryPlants both produce and utilize carbohydrates and have developed mechanisms to regulate their sugar status and co-ordinate carbohydrate partitioning. High sugar levels result in a feedback inhibition of photosynthesis and an induction of storage processes. We used a genetic approach to isolate components of the signalling pathway regulating the induction of starch biosynthesis. The regulatory sequences of the sugar inducible ADP-glucose pyrophosphorylase subunit ApL3 were fused to a negative selection marker. Of the four impaired sucrose induction (isi) mutants described here, two (isi1 and isi2) were speci®c to this screen. The other two mutants (isi3 and isi4) showed additional phenotypes associated with sugarsensing screens that select for seedling establishment on high-sugar media. The isi3 and isi4 mutants were found to be involved in the abscisic acid signalling pathway. isi3 is allelic to abscisic acid insensitive4 (abi4), a gene encoding an Apetala2-type transcription factor; isi4 was found to be allelic to glucose insensitive1 (gin1) previously reported to reveal cross-talk between ethylene and glucose signalling. Here we present an alternative interpretation of gin1 as an allele of the ABA-de®cient mutant aba2. Expression analysis showed that ABA is unable to induce ApL3 gene expression by itself, but greatly enhances ApL3 induction by sugar. Our data suggest a major role for ABA in relation to sugar-signalling pathways, in that it enhances the ability of tissues to respond to subsequent sugar signals.

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Section: Discussionmentioning

confidence: 99%

Impaired sucrose‐induction mutants reveal the modulation of sugar‐induced starch biosynthetic gene expression by abscisic acid signalling

Rook¹,

Corke²,

Card³

et al. 2001

The Plant Journal

375

351

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“…The following Arabidopsis thaliana T-DNA insertion mutants were used in this study: bam1 (Salk_039895), bam3 (CS92461) (Fulton et al, 2008), amy3-2 (Sail_613 D12) , amy3 bam1 (Horrer et al, 2016), snrk2.2 snrk2.3 snrk2.6 (Fujii and Zhu, 2009), areb1 areb2 abf3 (Yoshida et al, 2010), aao3-4 (Salk_072361) (Seo et al, 2004), nced3 (GABI_129B08) (Wan and Li, 2006), and aba2-1 (G1464A) (Léon-Kloosterziel et al, 1996). Arabidopsis ecotype Columbia-0 (Col-0) was used as the wild type in all experiments.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants

et al. 2016

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Starch serves functions that range over a timescale of minutes to years, according to the cell type from which it is derived. In guard cells, starch is rapidly mobilized by the synergistic action of b-AMYLASE1 (BAM1) and a-AMYLASE3 (AMY3) to promote stomatal opening. In the leaves, starch typically accumulates gradually during the day and is degraded at night by BAM3 to support heterotrophic metabolism. During osmotic stress, starch is degraded in the light by stress-activated BAM1 to release sugar and sugar-derived osmolytes. Here, we report that AMY3 is also involved in stress-induced starch degradation. Recently isolated Arabidopsis thaliana amy3 bam1 double mutants are hypersensitive to osmotic stress, showing impaired root growth. amy3 bam1 plants close their stomata under osmotic stress at similar rates as the wild type but fail to mobilize starch in the leaves. 14 C labeling showed that amy3 bam1 plants have reduced carbon export to the root, affecting osmolyte accumulation and root growth during stress. Using genetic approaches, we further demonstrate that abscisic acid controls the activity of BAM1 and AMY3 in leaves under osmotic stress through the AREB/ABF-SnRK2 kinase-signaling pathway. We propose that differential regulation and isoform subfunctionalization define starch-adaptive plasticity, ensuring an optimal carbon supply for continued growth under an ever-changing environment.

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“…The absence of ABA-induced dormancy allows seeds to germinate without gibberellins. Therefore, the selection of mutants that germinate in the presence of GA biosynthesis inhibitors, such as paclobutrazol and tetcyclacis, is an effective way to isolate ABA biosynthesis mutants (Léon- Kloosterziel et al, 1996b). Reciprocal crosses between wild type and the ABA deficient aba1 mutants showed that dormancy is controlled by the ABA genotype of the embryo and not by that of the mother plant.…”

Section: Genes and Factors Affected In Seed Dormancy/germinationmentioning

confidence: 99%

Seed dormancy and germination

Koornneef

Bentsink

Hilhorst

2002

Current Opinion in Plant Biology

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Isolation and characterization of abscisic acid‐deficient Arabidopsis mutants at two new loci

Cited by 445 publications

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Impaired sucrose‐induction mutants reveal the modulation of sugar‐induced starch biosynthetic gene expression by abscisic acid signalling

Impaired sucrose‐induction mutants reveal the modulation of sugar‐induced starch biosynthetic gene expression by abscisic acid signalling

Regulation of Leaf Starch Degradation by Abscisic Acid Is Important for Osmotic Stress Tolerance in Plants

Seed dormancy and germination

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