Isolation and characterization of actinomycetes from Brazilian tropical soils

Semêdo, L.T.A.S.; Linhares, A. A.; Gomes, Rosana Canuto; Manfio, Gilson Paulo; Alviano, Celuta Sales; Linhares, L. F.; Coelho, R. R. R.

doi:10.1016/s0944-5013(01)80007-0

Cited by 33 publications

(23 citation statements)

References 20 publications

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“…A number of marine organisms including actinomycetes, which live in marine environment, are inadequately understood and only few reports are available pertaining to marine oriented microorganisms (Siva, 2011). The actinomycetes are an important part of the microbial community in the soil environment, responsible for degradation and recycling of natural biopolymers, such as cellulose, lignin and chitin (Semedo et al, 2001). The species belonging to the genus Streptomyces constitute 50% of the total population of soil actinomycetes and are well known for producing a variety of bioactive secondary metabolites including antibiotics, immunomodulators, anticancer & antiviral drugs, herbicides, and insecticides (Rahman et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antifungal, Hemolytic and Cytotoxic Potential of Ethyl Acetate Extract of a New Marine Streptomyces sp. AIAH-10

et al. 2016

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To face newly generated diseases, search for new, safe and effective bioactive molecules is highly warranted. The marine microbial flora may be a potential source of such molecules. The present study was designed to isolate marine microorganisms (AIAH-1 to AIAH-29) from the soil of mangrove forest Sundarbans, Bangladesh by serial dilution method using isolation media. Among them, AIAH-10 was selected for further study due to its promising antibacterial activity (done by streak plate and plug technique method) against a series of pathogenic bacteria. On the basis of morphological, cultural and biochemical analysis, the strain AIAH-10 belongs to Streptomyces sp. Secondary metabolites of the strain was obtained by small scale fermentation process. Antifungal activity of the extracts was performed by disc diffusion method. The crude ethyl acetate extract (50 g/disc) showed significant antifungal activity against Aspergillus niger, Candida albicans and Saccharromyces cerevaceae (14, 12 and 10 mm zone of inhibition respectively). No hemolytic activity was found of the extracts towards the human erythrocytes. During cytotoxic study against brine shrimp nauplii (Artemia salina), a dose dependent mortality rate was observed. A 100% larva mortality rate was recorded in 40 g/ml and more where as LC50 was found to be 6.61 g/ml.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Antifungal, Hemolytic and Cytotoxic Potential of Ethyl Acetate Extract of a New Marine Streptomyces sp. AIAH-10

et al. 2016

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“…This strain was originally isolated from soil of the Atlantic Forest, Vista Chinesa, RJ [23], and characterized as belonging to the Streptomyces genus [22]. The molecular characterization by PCR amplification of the rrs gene had 99.0% identity with rrs gene sequences of Streptomyces lunalinharesii RCQ1071.…”

Section: Discussionmentioning

confidence: 99%

“…The genus and the origin of these bacterial strains are presented in Table 1. They were previously selected as promising for the production of AMSs against different microbial strains [22–25] and now were used to test their capability to produce AMSs with antimicrobial activity against aerobic and anaerobic bacteria associated with biocorrosion processes. Unless otherwise stated, these strains were grown in yeast extract-malt extract-agar (YMA) [26] under aerobic conditions at 28°C for 7 days.…”

Section: Methodsmentioning

confidence: 99%

Streptomyces lunalinharesiiStrain 235 Shows the Potential to Inhibit Bacteria Involved in Biocorrosion Processes

Rosa

Korenblum²,

Franco-Cirigliano³

et al. 2013

BioMed Research International

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Four actinomycete strains previously isolated from Brazilian soils were tested for their antimicrobial activity against Bacillus pumilus LF-4 and Desulfovibrio alaskensis NCIMB 13491, bacteria that are well known to be involved in biofilm formation and biocorrosion. Strain 235, belonging to the species Streptomyces lunalinharesii, inhibited the growth of both bacteria. The antimicrobial activity was seen over a wide range of pH, and after treatment with several chemicals and heat but not with proteinase K and trypsin. The antimicrobial substances present in the concentrated supernatant from growth media were partially characterized by SDS-PAGE and extracellular polypeptides were seen. Bands in the size range of 12 to 14.4 kDa caused antimicrobial activity. Transmission electron microscopy of D. alaskensis cells treated with the concentrated supernatant containing the antimicrobial substances revealed the formation of prominent bubbles, the spherical double-layered structures on the cell membrane, and the periplasmic space completely filled with electron-dense material. This is the first report on the production of antimicrobial substances by actinomycetes against bacteria involved in biocorrosion processes, and these findings may be of great relevance as an alternative source of biocides to those currently employed in the petroleum industry.

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“…São encontradas em diversos ambientes e são importantes componentes da população microbiana na maioria dos solos (Semêdo et al, 2001;Jayasinghe e Parkinson, 2008;Silva et al, 2012;Velayudham e Murungan, 2012 2013). O crescimento deste grupo é caracterizado pela formação de hifas e de um micélio aéreo ramificado, apresentando ampla diversidade morfológica (Ventura et al, 2007).…”

Section: Introductionunclassified