Isolation and characterization of alginate-degrading bacteria Sinomicrobium oceani

Jegatheesan, A.; Sudhakar, M.; Poonam, C.; Perumal, K.; Arunkumar, K.

doi:10.1007/s13399-016-0212-z

Cited by 4 publications

(3 citation statements)

References 45 publications

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“…Chitosan (Sigma-Aldrich, USA), a derivative of chitin found in the exoskeleton of shellfish, was provided at a concentration of 4 g L -1 (Gobet et al 2018). Alginate (sodium salt of alginate, Himedia), found in the cell wall of marine macroalgae belonging to the family Phaeophyceae (brown algae), was added a concentration of 10 g L -1 (Jegatheesan et al 2017). Cellulose (sodium salt of carboxymethylcellulose, Himedia), the main component of the cell wall of land plants, was provided at a concentration of 5 g L -1 (Olaniyi et al 2019).…”

Section: Methodsmentioning

confidence: 99%

Extremely oligotrophic and complex carbon degrading microaerobic bacteria from Arabian Sea oxygen minimum zone sediments

Sarkar,

Mondal,

Bhattacharya

et al. 2023

Preprint

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Sediments underlying marine hypoxias are huge sinks of unreacted complex organic matter, where despite acute O2-limitation aerobic bacterial communities thrive, and near-complete depletion of organic carbon takes place within a few meters below the seafloor. However, little knowledge exists about how aerobic chemoorganotrophs survive in these sulfidic ecosystems, and what may be their potentials for degrading complex carbon compounds. To elucidate these questions, we isolated and characterized a number of aerobic bacterial chemoorganoheterotrophs from across a ∼3-m sediment horizon underlying the perennial hypoxia of the eastern Arabian Sea. High levels of sequence correspondence between the isolates’ genomes and the habitat’s metagenomes and metatranscriptomes illustrated that the strains were widespread and active across the sediment cores explored. The isolates could catabolize several complex organic compounds of marine and terrestrial origins, via aerobic respiration at high as well as low O2concentrations. Some of them could also grow anaerobically on yeast extract or acetate by reducing nitrate and/or nitrite. Fermentation did not support growth in any of the strains, but enabled all of them to maintain a fraction of the cell population amidst prolonged anoxia. Under extreme oligotrophy, robust growth followed by protracted stationary phase was observed for all the isolates at low cell density, irrespective of whether O2was high or low. While metabolic deceleration was apparently central to the strains’ adaptation to dwindling organic carbon, O2-limitation could be potentially surmounted via supplies from known producers of biogenic O2, such asNitrosopumilusspecies, which had copious footprints in the habitat’s metagenomes.

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Section: Methodsmentioning

confidence: 99%

Extremely oligotrophic and complex carbon degrading microaerobic bacteria from Arabian Sea oxygen minimum zone sediments

Sarkar,

Mondal,

Bhattacharya

et al. 2023

Preprint

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Section: Testing Complex Organic Compound Utilizationmentioning

confidence: 99%

Extremely oligotrophic and complex-carbon-degrading microaerobic bacteria from Arabian Sea oxygen minimum zone sediments

Sarkar,

Mondal,

Bhattacharya

et al. 2024

Arch Microbiol

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Sediments underlying marine hypoxias are huge sinks of unreacted complex organic matter, where despite acute O2-limitation aerobic bacterial communities thrive, and near-complete depletion of organic carbon takes place within a few meters below the seafloor. However, little knowledge exists about how aerobic chemoorganotrophs survive in these sulfidic ecosystems, and what may be their potentials for degrading complex carbon compounds. To elucidate these questions, we isolated and characterized a number of aerobic bacterial chemoorganoheterotrophs from across a ~3-m sediment horizon underlying the perennial hypoxia of the eastern Arabian Sea. High levels of sequence correspondence between the isolates' genomes and the habitat's metagenomes and metatranscriptomes illustrated that the strains were widespread and active across the sediment cores explored. The isolates could catabolize several complex organic compounds of marine and terrestrial origins, via aerobic respiration at high as well as low O2 concentrations. Some of them could also grow anaerobically on yeast extract or acetate by reducing nitrate and/or nitrite. Fermentation did not support growth in any of the strains, but enabled all of them to maintain a fraction of the cell population amidst prolonged anoxia. Under extreme oligotrophy, robust growth followed by

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“…The biological pretreatment applied in several macroalgae, including the use of enzymes such as alginate lyases, laminarinases, or glucanases, for the degradation of polysaccharides in brown algae, were obtained from marine microorganisms [ 24 , 25 , 26 ] or seaweed composts [ 26 ]. The phenolic compounds and lignin [ 8 ] commonly found in Sargassum spp.…”

Section: Introductionmentioning

confidence: 99%

Improvement in Methane Production from Pelagic Sargassum Using Combined Pretreatments

Chikani-Cabrera¹,

Fernandes

Tapia-Tussell³

et al. 2022

Life

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The constant golden tides of Sargassum spp., identified to be a mixture of Sargassum natans and Sargassum fluitans, observed recently in the Mexican Caribbean have affected the marine ecosystem and the local economy and have created the need for solutions for their management and use. The Sargassum arrivals have thus been considered as third-generation feedstock for biofuel. Their potential for energetic conversion to biomethane was investigated, with hydrolysis as the limiting step due to its complex composition; therefore, in the present study, different physical, chemical, and enzymatic pretreatments and a combination of them have been evaluated, with the additional use of granular activated carbon, to determine the best yield and methane quality. The combined pretreatments of 2.5% hydrogen peroxide, followed by an enzymatic pretreatment (enzymatic extract from Trametes hirsuta isolated from decomposing wood in the Yucatán Peninsula-Mexico), was the best option, reaching a biodegradability of 95% and maximum methane yield of 387 ± 3.09 L CH4/kg volatile solid. The use of a conductive material, such as granular activated carbon, did not generate significant changes in performance and methane concentration.

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Isolation and characterization of alginate-degrading bacteria Sinomicrobium oceani

Cited by 4 publications

References 45 publications

Extremely oligotrophic and complex carbon degrading microaerobic bacteria from Arabian Sea oxygen minimum zone sediments

Extremely oligotrophic and complex carbon degrading microaerobic bacteria from Arabian Sea oxygen minimum zone sediments

Extremely oligotrophic and complex-carbon-degrading microaerobic bacteria from Arabian Sea oxygen minimum zone sediments

Improvement in Methane Production from Pelagic Sargassum Using Combined Pretreatments

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