Isolation and Characterization of Amylase-Producing Amylolytic Bacteria from Rice Soil Samples

Silaban, Saronom; Marika, Dona Best; Simorangkir, Murniaty

doi:10.1088/1742-6596/1485/1/012006

Cited by 11 publications

(8 citation statements)

References 5 publications

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“…When examined under a microscope, the results of endospore staining in both B. subtilis (UPMB10) and the mixed strains of bacteria reveal a dark green colour, indicating the presence of endospores. An endospore is a structure that can withstand harsh environmental conditions such as dryness, heat, and acidity (Rattray et al, 2021;Silaban et al, 2020). Endospores are extremely thick and refractile due to their low water content (Tehri et al, 2018).…”

Section: Bacillus Subtilis Endospores Detection After 12 Months Of Sh...mentioning

confidence: 99%

Humic Acid-Amended Formulation Improves Shelf-Life of Plant Growth-Promoting Rhizobacteria (PGPR) Under Laboratory Conditions

Sadeq¹,

Zuan²,

Kasim³

et al. 2023

JST

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Plant growth-promoting rhizobacteria (PGPR) is a soil bacterium that positively impacts soil and crops. These microbes invade plant roots, promote plant growth, and improve crop yield production. Bacillus subtilis is a type of PGPR with a short shelf-life due to its structural and cellular components, with a non-producing resistance structure (spores). Therefore, optimum formulations must be developed to prolong the bacterial shelf-life by adding humic acid (HA) as an amendment that could benefit the microbes by providing shelter and carbon sources for bacteria. Thus, a study was undertaken to develop a biofertilizer formulation from locally isolated PGPR, using HA as an amendment. Four doses of HA (0, 0.01, 0.05, and 0.1%) were added to tryptic soy broth (TSB) media and inoculated with B. subtilis (UPMB10), Bacillus tequilensis (UPMRB9) and the combination of both strains. The shelf-life was recorded, and viable cells count and optical density were used to determine the bacterial population and growth trend at monthly intervals and endospores detection using the malachite green staining method. After 12 months of incubation, TSB amended with 0.1% HA recorded the highest bacterial population significantly with inoculation of UPMRB9, followed by mixed strains and UPMB10 at 1.8x107 CFUmL-1, 2.8x107 CFUmL-1and 8.9x106 CFUmL-1, respectively. Results showed that a higher concentration of HA has successfully prolonged the bacterial shelf-life with minimal cell loss. Thus, this study has shown that the optimum concentration of humic acid can extend the bacterial shelf-life and improve the quality of a biofertilizer.

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Section: Bacillus Subtilis Endospores Detection After 12 Months Of Sh...mentioning

confidence: 99%

Humic Acid-Amended Formulation Improves Shelf-Life of Plant Growth-Promoting Rhizobacteria (PGPR) Under Laboratory Conditions

Sadeq¹,

Zuan²,

Kasim³

et al. 2023

JST

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“…This remains a substantial difference in the kinds of indigenous microorganisms extracted from distinct different host plants. Bacterial isolates are distinguished by their colors and colony shape [42,43]. A previous study was revealed the identification of BE from tomato [44],…”

Section: Diversity Of Bacteria From Citrusmentioning

confidence: 99%

Isolation and Identification of taxonomically diverse bacterial endophytes from citrus in Punjab Pakistan

Atta¹

2022

Preprint

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<pre style="margin: 0in 0in 0.0001pt; font-size: 10pt; font-family: "Courier New"; caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0); text-align: justify; line-height: 26.666664123535156px;"> Citrus is an economically important fruit crop grown in all provinces of Pakistan, while Punjab accounting for 95 percent of total production due to its favorable climate for citrus production. Commercially grown varieties in Pakistan include sweet oranges, grapefruits, Mandarine, Lime, and lemon. The goal of this research was to see how diverse the cultivable bacterial populations are found in citrus cultivars. Out of 90 isolated cultures, 37 endophytic bacterial species and 15 different genera of bacteria were characterized based on morphological, biochemical, and molecular methods from citrus leaves. All the isolated bacteria were subjected to PCR amplification through 16S rDNA followed by sequencing. RDP base classification revealed that class Bacilli has the largest percentage of isolates, whereas class Alpha, Beta, and Gamma Proteobacteria have the lowest percentage among all genotypes used. According to the findings, the phylum Firmicutes contains a common genus (Brevibacterium, 1%; Enterococcus, 6%; Staphylococcus, 7% and Bacillus, 60%). Alpha (Rhizobium) beta (Burkholderia cepacia; Comamonas terigena) gamma Proteobacteria (Enterobacter hermachei (1%), Klebsiella pneumoniae (1%), Proteus mirabilis (8%), Pseudomonas aeruginosa (5%), Psychrobacter pulmonis and Yersinia molalretti (1%) respectively. These results revealed that cultivars of the plants might contribute to the structure and endophytic bacterial communities associated with citrus. Endophytes extracted from leaf samples of different citrus cultivars in Pakistan are reported for the first time. The idea of employing endophytes bacteria to produce enzymes stimulate plant growth, and its purpose as a biological control agent will be investigated in the future.<o:p></o:p></pre>

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“…Amylases are a group of hydrolytic enzymes which hydrolyze the glycosidic linkages of starch and related polysaccharide molecules to produce simpler sugar molecules, such as glucose, maltose, and dextrin (My et al 2022;Silaban et al 2020). They are classified into three types comprising -amylase (1,4-α-D-glucan glucanohydrolase or glycogenase), -amylase (1,4-α-D-glucan maltohydrolase, glycogenase, or saccharogen amylase), and -amylase (glucan 1,4-α-glucosidase, amyloglucosidase, exo-1,4-α-glucosidase, glucoamylase, lysosomal αglucosidase, or 1,4-α-D-glucan glucohydrolase) based on the different mechanisms of the glycosidic linkage hydrolysis (Benjamin et al 2013;Joshi et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Diversity and activity of amylase-producing bacteria isolated from mangrove soil in Thailand

KLINFOONG¹,

THUMMAKASORN²,

UNGWIWATKUL³

et al. 2022

Biodiversitas

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Abstract. Klinfoong R, Thummakasorn C, Ungwiwatkul S, Boontanom P, Chantarasiri A. 2022. Diversity and activity of amylase-producing bacteria isolated from mangrove soil in Thailand. Biodiversitas 23: 5519-5531. Mangrove forests are a potential ecosystem for the isolation of various economic enzymes derived from mangrove-associated bacteria. The knowledge of amylase-producing bacteria isolated from mangrove forests in the Southeast Asian region has been scarce. This study aimed to investigate the isolation, genetic identification, and activity characterization of amylase-producing bacteria from mangrove soils in Thailand. The amylase-producing bacteria isolated from mangrove soils in the present were genetically belong to the genera Bacillus, Desulfurella, Peribacillus, Priestia, and Pseudomonas. Several amylase-producing bacteria such as Bacillus proteolyticus, Desulfurella, Pseudomonas entomophila, and Pseudomonas putida found in this study have hardly ever been reported. The Bacillus paralicheniformis strain DNP0507 was the most active amylolytic bacterium with 2.395 ± 0.133 U/mg of amylase activity. The optimum temperature and pH for amylolytic activity were determined to be 50°C at a pH of 7.0 with a thermal stability range of 20-60°C at a neutral pH of 7.0-8.0. The enzyme activity was significantly enhanced by Cu2+, Co2+, and Pb2+ and was inhibited considerably by a chelating agent EDTA. Finally, the most active amylolytic B. paralicheniformis strain DNP0507 could be applied in baking industries, food industries, and starchy waste valorization.

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Isolation and Characterization of Amylase-Producing Amylolytic Bacteria from Rice Soil Samples

Cited by 11 publications

References 5 publications

Humic Acid-Amended Formulation Improves Shelf-Life of Plant Growth-Promoting Rhizobacteria (PGPR) Under Laboratory Conditions

Humic Acid-Amended Formulation Improves Shelf-Life of Plant Growth-Promoting Rhizobacteria (PGPR) Under Laboratory Conditions

Isolation and Identification of taxonomically diverse bacterial endophytes from citrus in Punjab Pakistan

Diversity and activity of amylase-producing bacteria isolated from mangrove soil in Thailand

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