Isolation and characterization of an anti-complementary polysaccharide D3-S1 from the roots of Bupleurum smithii

Han, Xu; Zhang, Yunyi; Zhang, Jian‐Wen; Chen, Daofeng

doi:10.1016/j.intimp.2006.09.006

Cited by 78 publications

(49 citation statements)

References 27 publications

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“…Likewise, a similar protein-binding property could enable Tansy polysaccharides to bind or fix complement protein, thereby preventing formation of MAC and inhibiting hemolysis. Indeed, Xu et al [29] recently found that interaction of polysaccharide from Bupleurum smithii with complement proteins resulted in anti-complement activity. The complement system plays an essential role in innate immunity, contributing to inflammatory responses and the destruction and removal of pathogens (reviewed in [30]).…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory activity of acidic polysaccharides isolated from Tanacetum vulgare L.

Xie

Schepetkin

Quinn

2007

International Immunopharmacology

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Tanacetum vulgare L. (Tansy) has been extensively used in folk medicine for treatment of a variety of medical disorders. In the present study, we isolated and purified four acidic polysaccharide fractions (designated T-I to T-IV) from Tansy florets by the sequential use of hot-water extraction, ethanol precipitation, ultra-filtration, anion-exchange, and size-exclusion chromatography. The average M r of fractions T-I through T-IV was estimated to be 326, 151, 64 and 9 kDa, respectively, as determined by high performance size-exclusion chromatography analysis. Sugar composition analysis revealed that Tansy polysaccharides consisted primarily of galacturonic acid, galactose, arabinose, and rhamnose. Fractions T-II through T-IV contained an arabinogalactan type II structure, as determined by reaction with Yariv reagent. High M r fractions T-I and T-II exhibited potent macrophage/monocyte-activating activity, enhancing production of reactive oxygen species (ROS), nitric oxide (NO), and tumor necrosis factor α (TNF-α) by J774.A1 murine macrophages, and activating nuclear factor κB (NF-κB) in THP-1 human monocytes. In addition, Tansy polysaccharides stimulated human neutrophil function by greatly enhancing neutrophil myeloperoxidase (MPO) release. Furthermore, the low M r fraction T-IV had potent complementfixing activity, which may also contribute to the anti-inflammatory and would-healing properties of Tansy extracts. Taken together, our results provide a molecular basis to explain at least part of the beneficial therapeutic effects of Tansy extracts, and support the concept of using Tansy polysaccharides as an immunotherapeutic adjuvant.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory activity of acidic polysaccharides isolated from Tanacetum vulgare L.

Xie

Schepetkin

Quinn

2007

International Immunopharmacology

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“…showed a dose-dependently antiproliferative effect on the proliferation of A549 human lung cancer cells. Anticomplementary activity produced by the hot-water extract of the roots of Bupleurum smithii have also been found (Xu et al 2007). The major constituents, associated with the pharmacological activity of R. bupleuri chinensis, include triterpenoid glycosides of saikosaponin, essential oil, and polysaccharides (Tian et al 2009).…”

Section: Rhizoma Cimicifugaementioning

confidence: 95%

In vivo evaluation of anthelmintic potential of medicinal plant extracts against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

Zhu

Wang

et al. 2010

Parasitol Res

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In the present study, an attempt has been made to petroleum ether, chloroform, ethyl acetate, methanol, and water extracts of Prunus amygdalus Batsch seeds (Semen amygdali), Cimicifuga foetida L. rhizomes (Rhizoma Cimicifugae), Peucedanum decursivum (Miq.) Maxim roots (Radix Peucedani), Momordica cochinchinensis Spreng. seeds (Semen Momordicae), and Bupleurum chinense DC. roots (Radix Bupleuri chinensis) for their in vivo anthelmintic activity against monogenean Dactylogyrus intermedius in goldfish (Carassius auratus). The results showed that the efficacies of methanol, chloroform, and ethyl acetate extracts from R. Bupleuri chinensis were found to be, in this order, more effective than others with the 48 h-EC(50) and EC(90) values of 3.5 and 6.9, 6.0 and 8.4, 7.4 and 11.2 mg/L, respectively, followed by ethyl acetate extract of R. cimicifugae and chloroform extract of R. peucedani with EC(50) 189.2 and 240.4 mg/L. The promising methanol, chloroform, and ethyl acetate extracts from R. Bupleuri chinensis were subjected to acute toxicity tests for the evaluation of their safety to the host. After 48-h exposure, the mortalities of goldfish were recorded, and the established LC(50) values were 10.1-, 4.2-, and 8.4-fold higher than the corresponding EC(50). These results indicated that the three extracts from R. Bupleuri chinensis exhibit potential to be used as preferred natural antiparasitics for the control of the D. intermedius, especially for the methanolic one.

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“…[62][63][64] Free organic and fatty acids, like pinellic acid, angelic acid, petroselic acid and lignoceric acid, have also been identified in many species. [10] Other secondary metabolites, such as tannins, anthocyanidins and alkaloids, are absent in the genus, although a recent report claimed the presence of an indole-type alkaloidal glycoside (chaihuxinoside B) in the aerial parts of B. chinense.…”

Section: Other Compoundsmentioning

confidence: 99%

“…This inhibition was mediated through interaction with C1s, C3 and C4 complements, which are the major components of the innate immune system. [64] …”

Section: Immunomodulatory Activitymentioning

confidence: 99%

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Genus Bupleurum: a review of its phytochemistry, pharmacology and modes of action

Ashour

Wink

2011

Journal of Pharmacy and Pharmacology

229

161

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Objectives Radix Bupleuri represents one of the most successful and widely used herbal drugs in Asia for treatment of many diseases over the past 2000 years. Thorough studies have been carried out on many species of this genus and have generated immense data about the chemical composition and corresponding biological activity of extracts and isolated secondary metabolites. In this work, we review the chemistry and pharmacology of the genus Bupleurum and explore the relationships between the pharmacological effects and the chemical composition of these drugs. Key findings Early studies on the genus Bupleurum had focused only on the traditional uses of the plants in the treatment of inflammatory disorders and infectious diseases. After chemical profiling, several groups of secondary metabolites were characterized with relevant biological activity: triterpene saponins (saikosaponins), lignans, essential oils and polysaccharides. As a result, present interest is now focused on the bioactivity of the isolated triterpene saponins acting as immunomodulatory, anti-inflammatory and antiviral agents, as well as on the observed ant-iulcer activity of the polysaccharides and anti-proliferative activity of different lignans. Many saikosaponins exhibited very potent anti-inflammatory, hepatoprotective and immunomodulatory activities both in vivo and in vitro. Conclusions Further investigations and screenings are required to explore other Bupleurum species, to evaluate the clinical safety and possible interactions with other drugs or herbs. Standardization of Bupleuri extracts is crucial for them being integrated into conventional medicine due to large chemical and biological variations between different species and varieties.

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Isolation and characterization of an anti-complementary polysaccharide D3-S1 from the roots of Bupleurum smithii

Cited by 78 publications

References 27 publications

Immunomodulatory activity of acidic polysaccharides isolated from Tanacetum vulgare L.

Immunomodulatory activity of acidic polysaccharides isolated from Tanacetum vulgare L.

In vivo evaluation of anthelmintic potential of medicinal plant extracts against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

Genus Bupleurum: a review of its phytochemistry, pharmacology and modes of action

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