Isolation and characterization of an endophytic bacterium, Bacillus megaterium BMN1, associated with root-nodules of Medicago sativa L. growing in Al-Ahsaa region, Saudi Arabia

Khalifa, Ashraf; Almalki, Mohammed A.

doi:10.1007/s13213-014-0946-4

Cited by 30 publications

(31 citation statements)

References 46 publications

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“…Among different bacteria, various Bacillus species have been encountered as endophytes in symbiotic nodules of legumes, and some of them displayed plant growth promotion activity (Bai et al, ; Li et al, ; Stajković et al, ; Khalifa & Almalki, ). B. megaterium NMp082 presented specific characteristics that confer stress tolerance to abiotic stress in legume and non‐legume plants.…”

Section: Discussionmentioning

confidence: 99%

A nodule endophytic Bacillus megaterium strain isolated from Medicago polymorpha enhances growth, promotes nodulation by Ensifer medicae and alleviates salt stress in alfalfa plants

Appunu

Peña

Stoll

et al. 2018

Annals of Applied Biology

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A Gram-positive, fast-growing, endophytic bacterium was isolated from root nodules of Medicago polymorpha and identified as Bacillus megaterium. The isolate, named NMp082, co-inhabited nodules with the symbiotic rhizobium Ensifer medicae. B. megaterium NMp082 contained nifH and nodD genes that were 100% identical to those of Ensifer meliloti, an unusual event that suggested previous lateral gene transfer from a different rhizobial species. Despite the presence of nodulation and nitrogen fixation genes, the endophyte was not able to form effective nodules; however, it induced nodule-like unorganised structures in alfalfa roots. Axenic inoculation promoted plant growth in M. polymorpha, Medicago lupulina, Medicago truncatula and Medicago sativa, and co-inoculation with E. medicae enhanced growth and nodulation of Medicago spp. plants compared with inoculation with either bacterium alone. B. megaterium NMp082 also induced tolerance to salt stress in alfalfa and Arabidopsis plants. The ability to produce indole acetic acid (IAA) and the 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity displayed by the endophyte in vitro might explain the observed plant growth promotion and salt stress alleviation. The isolate was also highly tolerant to salt stress, water deficit and to the presence of different heavy metals. The newly characterised endophytic bacterium possessed specific characteristics that point at potential applications to sustain plant growth and nodulation under abiotic stress.A nodule endophyte enhances growth, nodulation and salt tolerance in alfalfa A. Chinnaswamy et al.

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Section: Discussionmentioning

confidence: 99%

A nodule endophytic Bacillus megaterium strain isolated from Medicago polymorpha enhances growth, promotes nodulation by Ensifer medicae and alleviates salt stress in alfalfa plants

Appunu

Peña

Stoll

et al. 2018

Annals of Applied Biology

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“…To the best of our knowledge, this is the first report of an endophytic nonrhizobial rhizobacteria being isolated from root nodules of Retama monosperma. Nevertheless, other Bacillus megaterium strains have already been identified in nodules of several leguminous species like the common bean (Phaseolus vulgaris L.) (Korir et al, 2017), pigeon pea (Cajanus cajan) (Rajendran et al, 2008), Medicago polymorpha (Chinnaswamy et al, 2018), and Medicago sativa L. (Khalifa and Almalki, 2015). More generally, a large and diverse set of nonrhizobial species live in legume root nodules in association with N 2 -fixing rhizobial species (Peix et al, 2014;Martínez-Hidalgo and Hirsch, 2017).…”

Section: B Megaterium Rmbm31 Induces Transcriptional Changes In Markmentioning

confidence: 99%

Unearthing the Plant Growth-Promoting Traits of Bacillus megaterium RmBm31, an Endophytic Bacterium Isolated From Root Nodules of Retama monosperma

et al. 2020

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Plants live in association with complex populations of microorganisms, including Plant Growth-Promoting Rhizobacteria (PGPR) that confer to plants an improved growth and enhanced stress tolerance. This large and diverse group includes endophytic bacteria that are able to colonize the internal tissues of plants. In the present study, we have isolated a nonrhizobial species from surface sterilized root nodules of Retama monosperma, a perennial leguminous species growing in poor and high salinity soils. Sequencing of its genome reveals this endophytic bacterium is a Bacillus megaterium strain (RmBm31) that possesses a wide range of genomic features linked to plant growth promotion. Furthermore, we show that RmBm31 is able to increase the biomass and positively modify the root architecture of seedlings of the model plant species Arabidopsis thaliana both in physical contact with its roots and via the production of volatile organic compounds. Lastly, we investigated the molecular mechanisms implicated in RmBm31 plant beneficial effects by carrying out a transcriptional analysis on a comprehensive set of phytohormone-responsive marker genes. Altogether, our results demonstrate that RmBm31 displays plant growth-promoting traits of potential interest for agricultural applications.

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“…In addition, plant growth-promoting rhizobacteria (e.g., Bacillus, Enterobacter, and Pseudomonas) have been isolated from some members of the Zygophyllaceae family (including Z. simplex) growing in the Al Taif region, and exhibited antagonistic activities against certain phytophaogens such as Fusarium oxysporum, and Sclerotinia sclerotiorum (El-Sayed et al,2014). Although several reports highlighted the benefits of rhizobacteria (Bibi et al, 2018, Khalifa and Almalki 2015, and Khalifa et al, 2016, a few reports investigated rhizobacteria in association with halophytes, particularly in the Al-Ahsa District. For example, rhiozbacteria associated with roots and root-nodules of Medicago sativa growing in Al-Ahsa region, enhanced growth of some grain legumes such as Pisum sativum Almalki 2015, andKhalifa et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Although several reports highlighted the benefits of rhizobacteria (Bibi et al, 2018, Khalifa and Almalki 2015, and Khalifa et al, 2016, a few reports investigated rhizobacteria in association with halophytes, particularly in the Al-Ahsa District. For example, rhiozbacteria associated with roots and root-nodules of Medicago sativa growing in Al-Ahsa region, enhanced growth of some grain legumes such as Pisum sativum Almalki 2015, andKhalifa et al, 2016). A few reports of ACC deaminase-producing PGPRs isolated from Al-Ahsa District, or the ability of native PGPRs to promote plant growth or ameliorate salt stress effects.…”

Section: Introductionmentioning

confidence: 99%

ACC Deaminase-Containing Rhizobacteria from Rhizosphere of Zygophyllum coccineum Alleviate Salt Stress Impact on Wheat (Triticum aestivum L.)

Khalifa¹

2020

SJKFU

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Plant growth-promoting bacteria are present in the rhizosphere, a soil zone around plant roots. An important mechanism of plant growth enhancement is the production of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Three ACC deaminase-producing bacterial strains were isolated from the rhizosphere of the halophytic plant Zygophyllum coccineum growing in Al-Uqair, Al-Ahsa, Saudi Arabia. The strains were identified as Bacillus filamentosus, Janibacter indicus, and Brevibacterium casei based on 16S rRNA sequence, and their taxonomic position was determined. Furthermore, the strains exhibited catalase and indoleacetic acid production and phosphate solubilization. The highest ACC deaminase activity was observed in B. filamentosus (~ 449 nmole α-ketobutyrate mg-1 h-1), followed by J. indicus and B. casei (~ 66 nmole α-ketobutyrate mg-1 h-1). Inoculation of wheat grains with each strain significantly enhanced growth. Z. coccineum rhizosphere harbors growth-promoting bacteria and ameliorated the negative effects of salt stress on wheat plants and may be used as an eco-friendly biofertilizer.

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Isolation and characterization of an endophytic bacterium, Bacillus megaterium BMN1, associated with root-nodules of Medicago sativa L. growing in Al-Ahsaa region, Saudi Arabia

Cited by 30 publications

References 46 publications

A nodule endophytic Bacillus megaterium strain isolated from Medicago polymorpha enhances growth, promotes nodulation by Ensifer medicae and alleviates salt stress in alfalfa plants

A nodule endophytic Bacillus megaterium strain isolated from Medicago polymorpha enhances growth, promotes nodulation by Ensifer medicae and alleviates salt stress in alfalfa plants

Unearthing the Plant Growth-Promoting Traits of Bacillus megaterium RmBm31, an Endophytic Bacterium Isolated From Root Nodules of Retama monosperma

ACC Deaminase-Containing Rhizobacteria from Rhizosphere of Zygophyllum coccineum Alleviate Salt Stress Impact on Wheat (Triticum aestivum L.)

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