Isolation and Characterization of Avian Chlamydia psittaci from Symptomatic Pet Birds in Southern Hunan, China

Wang, Chuan; Li, Li; Xie, Yanping; Tan, Yuan; Wen, Yang; Qian, Chen; Yu, Jiangnan; Song, Ying; Wu, Yimou

doi:10.1637/11932-071718-reg.1

Cited by 15 publications

(9 citation statements)

References 38 publications

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“…This indicated the infected poultry is very likely the origin of this cluster. This corresponds with the findings of recent surveys (Wang et al, 2019).…”

Section: Discussionsupporting

confidence: 93%

“…Previous published articles were used to guide the method of DNA extraction and C. psittaci specific real-time PCR targeting the 16 s rRNA gene and the ompA protein of C. psittaci (Messmer et al, 1997;Van Lent et al, 2012). All C. psittaci PCR-positive samples were re-analysed using real-time PCR amplification to obtain ompA sequences for genotyping using one pair of speciesspecific primers (CTU: 5′-ATGAAAAAACTCTTGAAATCGG-3′, and CTL: 5′-CAAGATTTTCTAGA(T/C)TTCAT(C/T)TTGTT--3′) (Wang et al, 2019). Cycling conditions were as previously described (Messmer et al, 1997;Van Lent et al, 2012).…”

Section: Real-time Pcr For the Detection And Sequencing Of Chlamydia ...mentioning

confidence: 99%

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A cluster of two psittacosis cases among women farmers exposed to Chlamydia psittaci‐infected domestic poultry in Zhejiang Province, China

Liu

Yang

Pang

et al. 2022

Zoonoses and Public Health

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A cluster of Chlamydia psittaci (C. psittaci) cases was reported in Zhejiang Province, China, 2019. This study evaluates the extent of the outbreak and determines the source of infection. Real-time PCR and sequencing of the ompA gene of C. psittaci were performed to identify the cases, the domesticated poultry and close contacts.The index patient was a 76-year-old woman with chronic vertigo, and Case 2 was a 64-year-old female farmer with herpes zoster. Both women bought psittaci-infected chickens or ducks from the same mobile street vendor and raised them for 10 days and 23 days before fever onset. There were no direct contact between the two women.C. psittaci test was positive for the two patients, one sick chicken, three healthy ducks and the vendor's chicken cage. Phylogenetic analysis showed that all seven C. psittaci positive samples carried identical ompA genotype A of C. psittaci. Of all of the patients' 148 close contacts, none tested positive for C. psittaci, or developed acute respiratory symptoms. Both patients were discharged after a 4-week hospital stay. In conclusion, the source of this cluster was the poultry infected with C. psittaci, which occasionally cause infections in farmers, but inter-human transmission seems unlikely.

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“…This indicated the infected poultry is very likely the origin of this cluster. This corresponds with the findings of recent surveys (Wang et al, 2019).…”

Section: Discussionsupporting

confidence: 93%

Section: Real-time Pcr For the Detection And Sequencing Of Chlamydia ...mentioning

confidence: 99%

A cluster of two psittacosis cases among women farmers exposed to Chlamydia psittaci‐infected domestic poultry in Zhejiang Province, China

Liu

Yang

Pang

et al. 2022

Zoonoses and Public Health

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“…Digestion was stopped by exposure to complete medium, HeLa cells were collected in a centrifuge tube at 4°C, 1000× rpm for 10 min. The supernatant was discarded, then add 200 mL of SPG solution (dodecahydrate disodium hydrogen phosphate 308 mg, potassium dihydrogen phosphate 52 mg, L-glutamic acid 72 mg, and sucrose 7500 mg, dissolved in 100 mL deionized water at pH 7.4-7.6, filtered and sterilized), EBs were then purified by density gradient centrifugation (35), and stored at −80°C until use.…”

Section: Psittaci Propagation and Purificationmentioning

confidence: 99%

The Chlamydia psittaci Inclusion Membrane Protein 0556 Inhibits Human Neutrophils Apoptosis Through PI3K/AKT and NF-κB Signaling Pathways

Xiao

Wang

et al. 2021

Front. Immunol.

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Inclusion membrane proteins (Incs) play an important role in the structure and stability of chlamydial inclusion and the interaction between Chlamydia spp. and their hosts. Following Chlamydia infection through the respiratory tract, human polymorphonuclear neutrophils (hPMN) not only act as the primary immune cells reaching the lungs, but also serve as reservoir for Chlamydia. We have previously identified a Chlamydia psittaci hypothetical protein, CPSIT_0556, as a medium expressed inclusion membrane protein. However, the role of inclusion membrane protein, CPSIT_0556 in regulating hPMN functions remains unknown. In the present study, we found that CPSIT_0556 could not only inhibit hPMN apoptosis through the PI3K/Akt and NF-κB signaling pathways by releasing IL-8, but also delays procaspase-3 processing and inhibits caspase-3 activity in hPMN. Up-regulating the expression of anti-apoptotic protein Mcl-1 and down-regulating the expression of pro-apoptotic protein Bax could also inhibit the translocalization of Bax in the cytoplasm into the mitochondria, as well as induce the transfer of p65 NF-κB from the cytoplasm to the nucleus. Overall, our findings demonstrate that CPSIT_0556 could inhibit hPMN apoptosis through PI3K/Akt and NF-κB pathways and provide new insights towards understanding a better understanding of the molecular pathogenesis and immune escape mechanisms of C. psittaci.

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“…Recently, an outbreak of Chlamydia -related egg production declines in domestic duck farms has been reported due to the infection of C. psittaci (genotype A) in Southeast of China [8]. In addition, this pathogen has also been detected and isolated occasionally from pet birds such as parrots [9]. These reports suggest domestic and companion birds might be a neglected reservoir of chlamydial pathogens which may threaten the poultry production and present risks to public health in China.…”

Section: Introductionmentioning

confidence: 99%

Detection of Chlamydia psittaci and Chlamydia ibidis in the Endangered Crested Ibis (Nipponia nippon)

Liu

Hou

et al. 2020

Epidemiol. Infect.

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Chlamydia spp. are a group of obligate intracellular pathogens causing a number of diseases in animals and humans. Avian chlamydiosis (AC), caused by Chlamydia psittaci (C. psittaci) as well as new emerging C. avium, C. gallinacea and C. ibidis, have been described in nearly 500 avian species worldwidely. The Crested Ibis (Nipponia nippon) is a world endangered avian species with limited population and vulnerable for various infections. To get a better understanding of the prevalence of Chlamydia spp. in the endangered Crested Ibis, faecal samples were collected and analysed. The results confirmed that 20.20% (20/99) of the faecal samples were positive for Chlamydiaceae and were identified as C. ibidis with co-existence of C. psittaci in one of the 20 positive samples. In addition, ompA sequence of C. psittaci obtained in this study was classified into the provisional genotype Matt116, while that of C. ibidis showed high genetic diversity, sharing only 77% identity with C. ibidis reference strain 10-1398/6. We report for the first time the presence of C. ibidis and C. psittaci in the Crested Ibis, which may indicate a potential threat to the endangered birds and should be aware of the future protection practice.

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Isolation and Characterization of Avian Chlamydia psittaci from Symptomatic Pet Birds in Southern Hunan, China

Cited by 15 publications

References 38 publications

A cluster of two psittacosis cases among women farmers exposed to Chlamydia psittaci‐infected domestic poultry in Zhejiang Province, China

A cluster of two psittacosis cases among women farmers exposed to Chlamydia psittaci‐infected domestic poultry in Zhejiang Province, China

The Chlamydia psittaci Inclusion Membrane Protein 0556 Inhibits Human Neutrophils Apoptosis Through PI3K/AKT and NF-κB Signaling Pathways

Detection of Chlamydia psittaci and Chlamydia ibidis in the Endangered Crested Ibis (Nipponia nippon)

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