Isolation and characterization of Azotobacter and Azospirillum strains from the sugarcane rhizosphere

Tejera, Noel A.; Lluch, Carmen; Martı́nez-Toledo, M.V.; González‐López, Jesús

doi:10.1007/s11104-004-1522-7

Cited by 129 publications

(80 citation statements)

References 47 publications

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“…In comparing the correlation between TNA and SNA of Azospirillum lipoferum, it revealed that they had correlation which was higher than that of the correlation between TNA and RNA. This differed from Tejera (2005) who found that azospirillum had high correlation in the root area and root surface of sugar cane. No other microbes were found at the surface and inside the root of sugar cane in Spain.…”

Section: Azotobacter Chroococcumcontrasting

confidence: 86%

“…It can enter root, consume surrounding organic substance, fix N 2 and release into the surrounding soil (Steenhoudt & Vanderleyden, 2000). From the study of Tejera (2005), it was found that azotobacter whose species are selected from soil (60%) and root (40%) revealed no close correlation, particularly in the area of fibrous root. No azotobacter was found inside the root of maize in the studying zone because most of azotobacter live freely and prevail in the soil at the appropriate temperature of 30…”

Section: The Effect Of Azotobacter Chroococcum and Azospirillum Lipofmentioning

confidence: 99%

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The Study on the Fixed Nitrogen and Nitrogenase Activity in the Day-Round of Azotobacter and Azosprillum Grown with Maize in KamphaengSaen Soil Series

Yamprai¹,

Mala²,

Sinma³

2014

MAS

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The aim of this study was to determine not only the activity of the nitrogenase enzyme, the fixed nitrogen in the day-round of Suwan 5 maize when inoculation with Azotobacter chroococcum and Azospirillum lipoferum but also the correlation between the nitrogenase activity and total fixed nitrogen. The statistical arrangement was 3 × 4 factorial in completely randomized design with 4 replications. The first factor was inoculation (Azotobacter chroococcum, Azospirillum lipoferum and non-inoculation (control)). The second factor was the observation time of nitrogenase activity (6:00, 12:00, 18:00, 24:00 hr.). The data were collected at 4 and 6 weeks. The results showed that the different time in a day-round did not affect the nitrogenase activity. Inoculation of azotobacter gave the highest total soil nitrogen at 4 and 6 week. (729.77 and 750.47 mg/ 10 kg-soil (while, the highest total plant nitrogen was found as inoculated with azospirillum (159.11 and 840.35 mg/plant of 4 and 6weeks). However, the total fixed nitrogen of azotobacter and azospirillum treatments were in the same level. Azotobacter treatment gave higher nitrogenase activity in soil than that of azospirillum treatment, while that in plant it was lower. The total nitrogenase activities of both inoculums were not different during the time of this study. The bulk (over all) correlation of total fixed nitrogen and total nitrogenase activity at week 4 and 6 were linear equations as y = 0.0224x + 18.045, R 2 = 0.8687 and y = 0.0117x + 14.57, R 2 = 0.7604 respectively.

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Section: Azotobacter Chroococcumcontrasting

confidence: 86%

Section: The Effect Of Azotobacter Chroococcum and Azospirillum Lipofmentioning

confidence: 99%

The Study on the Fixed Nitrogen and Nitrogenase Activity in the Day-Round of Azotobacter and Azosprillum Grown with Maize in KamphaengSaen Soil Series

Yamprai¹,

Mala²,

Sinma³

2014

MAS

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“…In general, variation and distribution of microorganisms is a reflection of soil fertility. As nitrogen is an essential element for plants, nitrogen-fixing bacteria would therefore provide molecular nitrogen for plant use [6]. Azotobacter species belong to the Gram-negative and the polymorphic family of Azotobacteraceae capable of forming capsule and microcyst [7].…”

mentioning

confidence: 99%

Identification of ten N2-fixing bacteria using 16S rRNA and their response to various zinc concentrations

Dadook¹,

Mehrabian²,

Irian³

2013

International Journal of Cellular and Molecular Biotechnology

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Zinc is presently being used as a micronutrient essential for plant growth in agriculture in the form of zinc sulphate in fertilizers. Considering the importance of nitrogen-fixing microorganisms in soil fertility and plant growth, and the toxic effects of zinc, this study aimed at isolating nitrogen-fixing microorganisms from the asparagus rhizosphere arable soil and examining the sensitivity of the isolated strains to different zinc concentrations. The asparagus rhizosphere soil was cultivated in a nitrogen-free environment at 30 °C for 48 hours. Strains were identified by both biochemical and molecular methods. The presence of the nitrogenase enzyme system was confirmed by testing for the presence of the nifH gene through PCR analysis. The minimum inhibitory concentration (MIC) and optimal zinc concentration for the growth of each strain were also determined. A total of 10 different bacterial species were identified in three different soil samples. The presence of the nifH gene was confirmed in seven different strains. MIC and the average optimal zinc concentration for bacterial growth were 130.7 ppm and 13 ppm, respectively. The asparagus rhizosphere soil contains different species of nitrogen stabilizing microorganisms. An optimal zinc concentration in soil of 20 ppm is suggested.

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“…The colony characters such as colony-margin, size, colour and consistency also differed among the different species of Azotobacter spp. (Tejera, et al 2005;Ahmad, et al 2008). The agronomic importance of Azotobacter spps is due to its the capability of biological nitrogen fixation, synthesis of antibiotics, plant growth hormones (Pandey et al, 1998), vitamins, exopolysaccharides and pigment (Jimenez et al, 2011) and also its antifungal activity (Sudhir et al, 1983).…”

Section: Characteristic Features Of Azotobacter Chroococcummentioning

confidence: 99%

Azotobacter chroococcum: Utilization and potential use for agricultural crop production: An overview

Sellamuthu¹,

Sathiya²,

Padaria³

2017

Int. J. Adv. Res. Biol. Sci

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It is important to develop integrated crop production strategies that enhance the productive ability of crops in a sustainable manner for the developing nations worldwide. Intensive farming practice of high yielding crop production is mainly dependent on chemical inputs as fertilizer, pesticide and weedicides. However, excessive use of chemical inputs has negative impact on environment in the form of soil infertility, ground water contamination, imbalance of biological ecosystem and cost inputs. Soil health is an important aspect of productive sustainable agriculture and in recent years maintaining soil quality can reduce the problems of land degradation, decreasing soil fertility through use of advanced technologies in the field of biofertilizers. Biofertilizers can produce mineral nutrients through natural processes of biological nitrogen fixation, phosphate solubilization and also stimulation plant growth through the synthesis of plant growth promoting substances. Thus, in recent years biofertilizers have become an important component of integrated management for enhanced crop production. This paper reviews the facts and observations regarding an important biofertilizer microorganism Azotobacter chroococcum and its potential for crop production based.

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Isolation and characterization of Azotobacter and Azospirillum strains from the sugarcane rhizosphere

Cited by 129 publications

References 47 publications

The Study on the Fixed Nitrogen and Nitrogenase Activity in the Day-Round of Azotobacter and Azosprillum Grown with Maize in KamphaengSaen Soil Series

The Study on the Fixed Nitrogen and Nitrogenase Activity in the Day-Round of Azotobacter and Azosprillum Grown with Maize in KamphaengSaen Soil Series

Identification of ten N2-fixing bacteria using 16S rRNA and their response to various zinc concentrations

Azotobacter chroococcum: Utilization and potential use for agricultural crop production: An overview

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